Molecular Study of Vancomycin Resistance in Hospital Acquired Staphylococcus Infection

Zaki, Magdi E. A.; Abouelnour, Aalaa; El-Kannishy, Sherif Mh; Hassan, Rasha M.

doi:10.36648/0975-9344.11.3.139

Int J Drug Dev & Res 2019

DOI: 10.36648/0975-9344.11.3.139

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Molecular Study of Vancomycin Resistance in Hospital Acquired Staphylococcus Infection

Magdi E. A. Zaki¹,

Aalaa Abouelnour²,

Sherif Mh El-Kannishy³

et al.

Abstract: In te rnation a l J o u rn al of Dr u g D e ve lopm e n t a n d Re sea rc h In e e n n t t

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2022

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Resistance profile of Staphylococcus strains and detection of the Mec A, Van A and Van B genes in private hospitals in Benin

Prosper¹,

Tamegnon²,

Koudokpon³

et al. 2022

Afr. J. Microbiol. Res.

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The present study aims to identify the emergence of Methicillin-resistant Staphylococcus aureus, S. aureus resistant to vancomycin and to investigate the presence of Mec A, Van A and Van B genes among Staphylococcus strains isolated from hospital environment. For each type of sample, surface of beds, recyclable material, boxes of rodar, floor and door slats, 53 samples were taken. So, a total of 265 samples were collected by swabbing (except boxes of rodar) in a private clinic in southern Benin. Bacteriological analysis was performed using the conventional method followed by DNA extraction with the Quiagen kit. The resistance genes Mec A, Van A and Van B were sought using specific primers. 215 samples were culture positive with 155 strains (62%) of coagulase negative (CNS) staphylococci and 95 strains (38%) of S. aureus. The majority of strains were resistant to gentamycin and clindamycin. These 155 strains were carried the Mec A gene and 10 strains carried the Van A and Van B gene. The study reveals the presence of resistant Staphylococci carrying the Mec A gene, which could be responsible for nosocomial infections in patients. Hygiene must be improved to limit the spread of these germs and protect patients.

show abstract

Resistance profile of Staphylococcus strains and detection of the Mec A, Van A and Van B genes in private hospitals in Benin

Prosper¹,

Tamegnon²,

Koudokpon³

et al. 2022

Afr. J. Microbiol. Res.

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show abstract

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Molecular Study of Vancomycin Resistance in Hospital Acquired Staphylococcus Infection

Abstract: In te rnation a l J o u rn al of Dr u g D e ve lopm e n t a n d Re sea rc h In e e n n t t

Cited by 1 publication

References 14 publications

Resistance profile of Staphylococcus strains and detection of the Mec A, Van A and Van B genes in private hospitals in Benin

Resistance profile of Staphylococcus strains and detection of the Mec A, Van A and Van B genes in private hospitals in Benin

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