Molecular surveillance and phylogenetic analysis of Theileria annulata in bovine at Baghdad city/ Iraq

Abstract

<jats:p>This study is conducted to investigate Theileria spp. by traditional and molecular methods. A total of 150 blood and 50 lymph samples were collected from local breed symptomatically and asymptomatically cattle of both sexes with age ranging from less than 6 months to more than 1 year during the four seasons of 2018, in different parts at Baghdad city / Iraq. Microscopic examination of Giemsa stained blood smears revealed 39.33 %( 59/150) rate of infection with bovine theileriosis and 34 %( 17/50) positive lymph smears. Statistically no significant difference recorded between female and male: 42.04 % (37/88) and 35.48 % (22/62) respectively. Higher rate of infection 57.97 % (40/69) were recorded in more than 1 year age and 0 % in less than 6 months. 48.93 % (23/47) rate of bovine theileriosis was recorded during summer and 39.53 % (17/43) ; 37.5 % (15/40) rates were recorded during spring and autumn respectively, while the lower rate recorded in winter 20 % (4/20). DNA extraction and polymerase chain reaction (conventional PCR) were done on all cattle blood samples the result recorded that 22 out of 25 samples were positive for Theileria spp and Theileria annulata with percentage of 88 %. Also DNA sequencing analysis and genetic relationship were conducted by phylogenetic analysis.</jats:p>

Prevalence and molecular identification of Nematodirus helvetianus in camels in Iraq

“…A general analysis of the genetic similarity using rRNA sequences data showed the greatest pairwise similarity between N. spathiger and N. helvetianus, Nematodirus battus, and the nucleotide sequence of ITS-2 rRNA (3.9-24.7%) [21]. The previous studies used the ITS1/ITS2 genes (ribosomal) as well as the cytochrome b gene (mitochondrial) as reliable molecular markers for different nematode and protozoan parasites in Iraqi camels and cattle [22]. This is the first morphological (male) and molecular (female) study to identify N. helvetianus in the Iraqi camel [23,24].…”

CONVENTIONAL AND MOLECULAR STUDY OF Entamoeba spp. IN DOMESTIC DOG’S FECAL AT BAGHDAD CITY, IRAQ.

“…Light microscope is used to detection of Entamoeba by wet smear but the results mostly difficult to distinguish the trophozoite and cyst of pathogenic E. histolytica from the cyst and trophozoite of non-pathogenic (16). Accurate molecular methods available PCR that can distinguish E. histolytica, E. moshkovskii, and E. dispar (9,3,20). A few is known about the molecular diagnosis of the species of this parasite in dogs in Iraq.…”