Molecular survey of canine vector-borne diseases in stray dogs in Thailand

Liu, Mingming; Ngasaman, Ruttayaporn; Saechan, Vannarat; Jirapattharasate, Charoonluk; Vudriko, Patrick; Moumouni, Paul Franck Adjou; Cao, Shinuo; Inpankaew, Tawin; Ybañez, Adrian P.; Suzuki, Hiroshi; Xuan, Xuenan

doi:10.1016/j.parint.2016.04.011

Cited by 58 publications

(58 citation statements)

References 23 publications

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“…The results of this study supported the previous report regarding the presence of the highest prevalence of E. canis (21.5%) then H. canis (10.1%) and B. canis vogeli (6.3%) in stray dogs in Mahasarakham, Thailand [18]. In the southern region of Thailand, occurrence of E. canis infection in stray dogs was the lowest at 3.9% [19]. The variations of all tickborne pathogen infection rate may have been caused by differences in sampling location, distribution of the tick vector, season and examination technique.…”

Section: Discussionsupporting

confidence: 90%

Molecular detection of blood pathogens and their impacts on levels of packed cell volume in stray dogs from Thailand

Piratae¹,

Saechue²,

Sukumolanan³

et al. 2017

APJTD

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Section: Discussionsupporting

confidence: 90%

Molecular detection of blood pathogens and their impacts on levels of packed cell volume in stray dogs from Thailand

Piratae¹,

Saechue²,

Sukumolanan³

et al. 2017

APJTD

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“…Coinfection occurred at 11.8% (Table-1). In Thailand, the percentage of A. platys is similar to that described in Maha Sarakham Province (29.2%) [11], but different from which reported in Songkhla (4.4%) [19], correlation with E. canis infection rate that is comparable to that described in Maha Sarakham Province (21.5%) [17], but dissimilar from which reported in Songkhla (3.9) [19]. However, these results might be explained by locality, where Maha Sarakham and Kalasin are adjoining provinces in the Northeastern part of Thailand may be similar in structure, climate, and humidity.…”

Section: Resultssupporting

confidence: 51%

Molecular evidence of Ehrlichia canis and Anaplasma platys and the association of infections with hematological responses in naturally infected dogs in Kalasin, Thailand

et al. 2019

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Background: Tick-borne bacteria, Anaplasma platys and Ehrlichia canis are well recognized as the etiology of anemia and thrombocytopenia in dogs. The clinical signs of anaplasmosis and ehrlichiosis range from asymptomatic to severe symptoms. There are insufficient studies about epidemiological surveys of these blood parasites, also the association of infections with the hematological study. Aim: This study aimed to screen A. platys and E. canis in naturally infected dogs and the effects of the infection on the levels of packed cell volume (PCV) and platelet count. Materials and Methods: A total of 68 blood samples were collected from free-roaming dogs at Nong Kung Sri district, Kalasin Province, Thailand, and examined for A. platys and E. canis infection by polymerase chain reaction (PCR) and measured PCV levels and platelet count. Results: Using nested PCR, 42.65% of dogs were infected with one or two pathogens. The molecular detection of anaplasmosis and ehrlichiosis in this population was 29.4% (95% confidence interval [CI]: 18.98-41.71) and 25% (95% CI: 14.4-35.3), respectively. Coinfection occurred at 11.8% (95% CI: 5.22-21.87). Infection with E. canis and coinfection showed significant association with PCV levels (p<0.05) while A. platys infection showed no statistical relationship. Infection with A. platys, E. canis, and coinfection had a non-significant correlation with platelet count (p>0.05). Conclusion: This study provides data of anaplasmosis and ehrlichiosis in free-roaming dogs which indicated that these zoonotic diseases are widespread and require for disease frequency determination, especially in Kalasin Province of Thailand where data of tick-borne infections in dogs have not been reported.

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“…16S rRNA metabarcoding is better able to elucidate diversity in explorative research by not relying on likely pathogen prevalence in a region nor on a priori knowledge of a pathogen's target genetic sequences, whilst also being better able to characterise coinfection [18,20]. Previous work has already developed a novel 16S and 18S rRNA metabarcoding methodology to characterise the range of blood-borne bacterial, apicomplexan and kinetoplastid organisms infecting canine hosts in Thailand, a country of substantial CVBD diversity [4,8,[21][22][23]. The current research takes this further, by tackling some of the inherent challenges of NGS microbiome research whilst also improving methods to be better at unearthing pathogen diversity in the context of the canine blood micro-environment [24].…”

Section: Introductionmentioning

confidence: 99%

A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria

et al. 2020

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Bacterial canine vector-borne diseases are responsible for some of the most life-threatening conditions of dogs in the tropics and are typically poorly researched with some presenting a zoonotic risk to cohabiting people. Next-generation sequencing based methodologies have been demonstrated to accurately characterise a diverse range of vector-borne bacteria in dogs, whilst also proving to be more sensitive than conventional PCR techniques. We report two improvements to a previously developed metabarcoding tool that increased the sensitivity and diversity of vector-borne bacteria detected from canine blood. Firstly, we developed and tested a canine-specific blocking primer that prevents cross-reactivity of bacterial primer amplification on abundant canine mitochondrial sequences. Use of our blocking primer increased the number of canine vector-borne infections detected (five more Ehrlichia canis and three more Anaplasma platys infections) and increased the diversity of bacterial sequences found. Secondly, the DNA extraction kit employed can have a significant effect on the bacterial community characterised. Therefore, we compared four different DNA extraction kits finding the Qiagen DNeasy Blood and Tissue Kit to be superior for detection of blood-borne bacteria, identifying nine more A. platys, two more E. canis, one more Mycoplasma haemocanis infection and more putative bacterial pathogens than the lowest performing kit.

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Molecular survey of canine vector-borne diseases in stray dogs in Thailand

Cited by 58 publications

References 23 publications

Molecular detection of blood pathogens and their impacts on levels of packed cell volume in stray dogs from Thailand

Molecular detection of blood pathogens and their impacts on levels of packed cell volume in stray dogs from Thailand

Molecular evidence of Ehrlichia canis and Anaplasma platys and the association of infections with hematological responses in naturally infected dogs in Kalasin, Thailand

A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria

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