Molecular Survey of RNA Viruses in Hungarian Bats: Discovering Novel Astroviruses, Coronaviruses, and Caliciviruses

Kemenesi, Gábor; Dallos, Bianka; Görföl, Tamás; Boldogh, Sándor; Estók, Péter; Kurucz, Kornélia; Kutas, Anna; Földes, Fanni; Oldal, Miklós; Németh, Viktória; Martella, Vito; Bányai, Krisztiàn; Jakab, Ferenc

doi:10.1089/vbz.2014.1637

Cited by 73 publications

(72 citation statements)

References 34 publications

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“…This work highlights the apparent high diversity of astroviruses circulating among bat populations in Lao PDR and Cambodia. This finding was consistent with previous reports (Chu et al, 2008;Zhu et al, 2009;Xiao et al, 2011;Wu et al, 2012;Hu et al, 2014;Kemenesi et al, 2014aKemenesi et al, , 2014bDufkova et al, 2015). In some sites, multiple viruses were found in bats from a single genus and collected during one sampling session, within one habitat, whereas in other cases bats from several different genera, sampled in different habitats at various times were found to have just one astV.…”

Section: Discussionsupporting

confidence: 93%

Diversity of bat astroviruses in Lao PDR and Cambodia

Lacroix

Duong

Hul

et al. 2017

Infection, Genetics and Evolution

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Astroviruses are known to infect humans and a wide range of animal species, and can cause gastroenteritis in their hosts. Recent studies have reported astroviruses in bats in Europe and in several locations in China. We sampled 1876 bats from 17 genera at 45 sites from 14 and 13 provinces in Cambodia and Lao PDR respectively, and tested them for astroviruses. Our study revealed a high diversity of astroviruses among various Yangochiroptera and Yinpterochiroptera bats. Evidence for varying degrees of host restriction for astroviruses in bats was found. Furthermore, additional Pteropodid hosts were detected. The astroviruses formed distinct phylogenetic clusters within the genus Mamastrovirus, most closely related to other known bat astroviruses. The astrovirus sequences were found to be highly saturated indicating that phylogenetic relationships should be interpreted carefully. An astrovirus clustering in a group with other viruses from diverse hosts, including from ungulates and porcupines, was found in a Rousettus bat. These findings suggest that diverse astroviruses can be found in many species of mammals, including bats.

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Section: Discussionsupporting

confidence: 93%

Diversity of bat astroviruses in Lao PDR and Cambodia

Lacroix

Duong

Hul

et al. 2017

Infection, Genetics and Evolution

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“…In Cambodia, an association between host and virus clade was observed between bats from the genus Myotis and the αCoVs genetically related to PEDV strains. These results are in line with conclusions of previous studies: all CoVs detected in bats belonging to the Myotis genus were always αCoVs (Tang et al, 2006;Woo et al, 2006;Dominguez et al, 2007;Gloza-Rausch et al, 2008;Osborne et al, 2011;August et al, 2012;Kemenesi et al, 2014;Fischer et al, 2016). βCoVs from lineage D have frequently been found in frugivorous bat species from Madagascar, Kenya, Thailand, and Hong Kong Anindita et al, 2015;Razanajatovo et al, 2015;Wacharapluesadee et al, 2015;Xu et al, 2016).…”

Section: Discussionsupporting

confidence: 92%

Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia

Lacroix

Duong

Hul

et al. 2017

Infection, Genetics and Evolution

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South-East Asia is a hot spot for emerging zoonotic diseases, and bats have been recognized as hosts for a large number of zoonotic viruses such as Severe Acute Respiratory Syndrome (SARS), responsible for acute respiratory syndrome outbreaks. Thus, it is important to expand our knowledge of the presence of viruses in bats which could represent a risk to humans. Coronaviruses (CoVs) have been reported in bat species from Thailand, China, Indonesia, Taiwan and the Philippines. However no such work was conducted in Cambodia or Lao PDR. Between 2010 and 2013, 1965 bats were therefore sampled at interfaces with human populations in these two countries. They were tested for the presence of coronavirus by consensus reverse transcription-PCR assay. A total of 93 samples (4.7%) from 17 genera of bats tested positive. Sequence analysis revealed the presence of potentially 37 and 56 coronavirus belonging to alpha-coronavirus (αCoV) and beta-CoV (βCoV), respectively. The βCoVs group is known to include some coronaviruses highly pathogenic to human, such as SARS-CoV and MERS-CoV. All coronavirus sequences generated from frugivorous bats (family Pteropodidae) (n=55) clustered with other bat βCoVs of lineage D, whereas one coronavirus from Pipistrellus coromandra fell in the lineage C of βCoVs which also includes the MERS-CoV. αCoVs were all detected in various genera of insectivorous bats and clustered with diverse bat αCoV sequences previously published. A closely related strain of PEDV, responsible for severe diarrhea in pigs (PEDV-CoV), was detected in 2 Myotis bats. We highlighted the presence and the high diversity of coronaviruses circulating in bats from Cambodia and Lao PDR. Three new bat genera and species were newly identified as host of coronaviruses, namely Macroglossus sp., Megaerops niphanae and Myotis horsfieldii.

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“…High sequence diversity is commonly described in bat AstVs, 3 with studies reporting potentially novel AstV species in these hosts. 9, 13, 14 The coalescent analysis provided further evidence of this considerable diversity and the low degree of host restriction (Figure 1). For instance, the two viruses detected in Rousettus madagascariensis were more genetically related to AstVs found in Miniopterus griveaudi and in Triaenops menamena than to each other.…”

mentioning

confidence: 82%

Astroviruses in bats, Madagascar

Lebarbenchon

Ramasindrazana

Joffrin

et al. 2017

Emerging Microbes & Infections

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International audienceDear Editor, Astroviruses (AstVs) (family Astroviridae) are positive-sense, single-stranded RNA viruses that infect a large diversity of mammalian and avian species. 1 In humans, eight serotypes have been described worldwide, accounting for 2%–9% of all acute non-bacterial gastro-enteritis cases in children. 2 AstVs have been detected from over 80 non-human host species, including a large diversity of bat species in Asia, Europe and Africa, 3 and recent phylogenetic analyses suggest that the long-term evolution of AstVs is determined by frequent cross-species transmission events. 4,5 During the past decade, numerous AstVs with potential zoonotic transmission have been described, highlighting the need for improved knowledge on their biology to prevent future health threats. 2 Bats are recognized as a major reservoir of infectious agents. Madagascar shelters over 46 bat species, of which nearly 80% are endemic, occupying different types of day roosts, including natural and synanthropic sites. Recent investigations have shown that the compositions of Malagasy bat species assemblages are correlated with factors associated with the diversity and transmission of infectious agents, such as paramyxoviruses and Leptospira. 6,7 The detection of new Betacoronaviruses in endemic Malagasy fruit bats also underlined the urgent need to better understand bat-associated infectious agents, and thus assess the potential for spillover to human populations in this major biodiversity hotspot.

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Molecular Survey of RNA Viruses in Hungarian Bats: Discovering Novel Astroviruses, Coronaviruses, and Caliciviruses

Cited by 73 publications

References 34 publications

Diversity of bat astroviruses in Lao PDR and Cambodia

Diversity of bat astroviruses in Lao PDR and Cambodia

Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia

Astroviruses in bats, Madagascar

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