Molecular systematics applied to Phlebotomine sandflies: Review and perspectives

Depaquit, Jérôme

doi:10.1016/j.meegid.2014.10.027

Cited by 68 publications

(40 citation statements)

References 159 publications

(389 reference statements)

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“…Thus the universe of available molecular data for the subfamily consists of isolated data sets that resist combination and thus a comprehensive analysis of its internal relationships. A thorough survey of all molecular data studies of phlebotomines has recently been done by Depaquit (), and confirms the initial observations that motivated our study here: although becoming voluminous, phlebotomine sequence data have come mostly from studies of populations and small species groups, and among them, mostly those of medical importance. In short, the sequence data have not been collected with the aim of resolving long‐standing questions in morphology‐based taxonomy.…”

Section: Introductionsupporting

confidence: 86%

A phylogeny of sand flies (Diptera: Psychodidae: Phlebotominae), using recent Ethiopian collections and a broad selection of publicly available DNA sequence data

Grace-Lema

Yared

Quitadamo

et al. 2015

Systematic Entomology

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Sand flies in the psychodid subfamily Phlebotominae carry important human pathogens in the trypanosomatid protozoan genus Leishmania (Cupolillo). Despite the fact that hundreds of sequences for this group are now publicly available, they constitute different sets of taxa and genetic markers. Integrating these data to construct a molecular phylogeny of the family is a significant bioinformatics challenge. We used sequences of eight markers obtained from freshly collected sand flies from Ethiopia and combined them with over 1300 publicly available sequences, performing a combined analysis after generating single terminal sequences from ancestral reconstructions for some individual markers. The resulting phylogeny had 113 terminals and recovered Phlebotominae and certain species groups as monopheletic. Although the 20 outgroups in Psychodinae were recovered as a well-resolved clade with bootstrap support for many internal clades, Phlebotominae was recovered as several lineages with unclear relationships among them. However, phlebotomines clustered by geographic region, the most notable being all the New World species except Brumptomyia (Galati), which were recovered as monophyletic. Our phylogeny suggests a Sub-Saharan African or South Asian origin for the subfamily, which subsequently expanded to the north and west, and eventually to the New World. Supported species groups are often composed of widespread species with overlapping ranges. This result highlights the need for a large increase in the amount and diversity of molecular sequence data, and a broad selection of terminals, to test taxonomic hypotheses and examine speciation processes in this important group of flies.

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Section: Introductionsupporting

confidence: 86%

A phylogeny of sand flies (Diptera: Psychodidae: Phlebotominae), using recent Ethiopian collections and a broad selection of publicly available DNA sequence data

Grace-Lema

Yared

Quitadamo

et al. 2015

Systematic Entomology

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“…COI has been a commonly used genetic marker for species identi cation since its introduction as "the barcoding gene" by Hebert et al [43] and thus, sequence availability in GenBank is high and COI is frequently used for sand y identi cation and interspeci c comparisons [44]. Although there is no common cut-off value for species delimitation, Hebert et al [43] observed a mean divergence value of 11.3% between species and only a small fraction showed 2% or less divergence.…”

Section: Discussionmentioning

confidence: 99%

“…To corroborate our results, cyt b was used as a second genetic marker, albeit sequence availability is rather poor for Adlerius species. Cyt b is the most used genetic marker in sand y systematics [44]. Further con rmation of species delimitation was achieved by comparing the obtained Ph.…”

Section: Discussionmentioning

confidence: 99%

Phlebotomus (Adlerius) Simici Nitzulescu, 1931: First Record in Austria and Phylogenetic Relationship With Other Adlerius Species

Kniha

Dvořák

Milchram

et al. 2020

Preprint

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Background Phlebotomine sand flies are the principal vectors of Leishmania spp. (Kinetoplastida: Trypanosomatidae). Sand fly findings in Central Europe are scarce and in Austria, to date only Phlebotomus mascittii has been recorded. In 2018 and 2019, entomological surveys were conducted in Austria with the aim to further clarify sand fly distribution and species composition. Results In 2019, a Ph. simici specimen was trapped in Austria for the first time. Analyses of two commonly used marker genes, COI and cyt b, revealed high sequence identity with Ph. simici specimens from North Macedonia and Greece. Phylogenetic analyses showed high intraspecific distances within Ph. simici, thereby dividing this species into three lineages, from Europe, Turkey and Israel, respectively. Low interspecific distances between Ph. simici, Ph. brevis and a yet unidentified Adlerius sp. from Turkey and Armenia highlights that molecular identification can be challenging within the Adlerius complex, even when applying standard marker genes. Conclusion This study provides the first finding of Ph. simici in Austria and the northernmost record so far. Moreover, it reveals valuable insights into the phylogenetic relationships of species within the Adlerius subgenus. Ph. simici is a suspected vector of Leishmania infantum and therefore of medical and veterinary importance. Potential sand fly expansion in Central Europe due to climatic change and the increasing import of Leishmania-infected dogs from endemic areas, urge the need for further studies on sand fly distribution in Austria and Central Europe in general.

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“…Molecular methods based on various genetic markers have been developed to overcome this issue (Depaquit ). So far, two techniques have been mainly used for molecular identifications of sand flies: the polymerase chain reaction‐restriction fragment‐length polymorphism (PCR‐RFLP; Aransay et al .…”

Section: Introductionmentioning

confidence: 99%

“…Molecular methods based on various genetic markers have been developed to overcome this issue (Depaquit 2014). So far, two techniques have been mainly used for molecular identifications of sand flies: the polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP; Aransay et al 1999;Terayama et al 2008;Latrofa et al 2012;Tiwary et al 2012;Minter et al 2013) and DNA barcoding (Kr€ uger et al 2011;Kumar et al 2012;Contreras Guti errez et al 2014;Al-Ajmi et al 2015;Maia et al 2015;Nzelu et al 2015;Romero-Ricardo et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Vector soup: high‐throughput identification of Neotropical phlebotomine sand flies using metabarcoding

Kocher

Gantier²,

Gaborit

et al. 2016

Molecular Ecology Resources

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Phlebotomine sand flies are haematophagous dipterans of primary medical importance. They represent the only proven vectors of leishmaniasis worldwide and are involved in the transmission of various other pathogens. Studying the ecology of sand flies is crucial to understand the epidemiology of leishmaniasis and further control this disease. A major limitation in this regard is that traditional morphological-based methods for sand fly species identifications are time-consuming and require taxonomic expertise. DNA metabarcoding holds great promise in overcoming this issue by allowing the identification of multiple species from a single bulk sample. Here, we assessed the reliability of a short insect metabarcode located in the mitochondrial 16S rRNA for the identification of Neotropical sand flies, and constructed a reference database for 40 species found in French Guiana. Then, we conducted a metabarcoding experiment on sand flies mixtures of known content and showed that the method allows an accurate identification of specimens in pools. Finally, we applied metabarcoding to field samples caught in a 1-ha forest plot in French Guiana. Besides providing reliable molecular data for species-level assignations of phlebotomine sand flies, our study proves the efficiency of metabarcoding based on the mitochondrial 16S rRNA for studying sand fly diversity from bulk samples. The application of this high-throughput identification procedure to field samples can provide great opportunities for vector monitoring and eco-epidemiological studies.

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Molecular systematics applied to Phlebotomine sandflies: Review and perspectives

Cited by 68 publications

References 159 publications

A phylogeny of sand flies (Diptera: Psychodidae: Phlebotominae), using recent Ethiopian collections and a broad selection of publicly available DNA sequence data

A phylogeny of sand flies (Diptera: Psychodidae: Phlebotominae), using recent Ethiopian collections and a broad selection of publicly available DNA sequence data

Phlebotomus (Adlerius) Simici Nitzulescu, 1931: First Record in Austria and Phylogenetic Relationship With Other Adlerius Species

Vector soup: high‐throughput identification of Neotropical phlebotomine sand flies using metabarcoding

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