Molecular Systematics of Australian Carrion-breeding Blowflies of the Genus Calliphora (Diptera: Calliphoridae)

Abstract

Allozyme electrophoresis was used to determine the systematic affinities of nine forms of carrion-breeding blowfly of the genus Calliphora: C. stygia, C. albifrontalis, C. augur, C. dubia, C. hilli hilli, C. hilli fallax, C. varifrons, C. sp. nov., and C. maritima. The results (1) confirm the species status of all forms currently described as such, (2) support a return to the ranking of C. hilli fallax as a full species, C. fallax, (3) support the recognition of C. sp. nov. as a distinct species, and (4) indicate that distinct Kangaroo Island and adjacent mainland subpopulations appear to exist in at least three species. The allozyme data also strongly support the placing of eight of the forms into three separate species-groups on morphological grounds, and the placement of C. maritima in a fourth group. However, on the basis of these data, the comparative genetic affinities of the parasitic blowfly Onesia tibialis suggest that Calliphora in its current form may be paraphyletic.

Phylogenetic analysis of forensically important Lucilia flies based on cytochrome oxidase I sequence: a cautionary tale for forensic species determination

“…2). The paraphyly of L. cuprina COI seen here was previously described [27,31]; however, in our earlier paper we thought that this was solely because of a distinct Hawaiian lineage with all other populations being monophyletic for mtDNA [27]. Based on these additional data, the Hawaiian L. cuprina haplotypes are not distinct as previously thought.…”

“…For most L. cuprina in our analyses, we lack the morphological data needed to distinguish these two forms. Therefore, we cannot judge whether they should be considered separate species as advocated by Wallman et al [31]. In contrast to this mtDNA phylogeny, L. cuprina was monophyletic for nuclear loci [23,24,27].…”

Rediscovery, redescription and reclassification of Beludzhia phylloteliptera (Diptera: Sarcophagidae: Miltogramminae)

“…One feature worthy of mention, however, is the occurrence of integumental warts (Figs 64,76). A similar cuticular sculpturing has not been reported from any other member of the Sarcophagidae and has been reported from the Oestroidea for the third instar larvae of following genera of blowflies: Calliphora Robineau-Desvoidy, 1830 (Erzinç-lio lu, 1985;Liu & Greenberg, 1989;Wallman, 2001), Chrysomya Robineau-Desvoidy, 1830 (Povolný, 2002;Sukontason et al, 2003), and Lucilia Robineau-Desvoidy, 1830 (Liu & Greenberg, 1989).…”

“…The SDF has significant diagnostic value in the third instar larvae of many Calliphoridae (Erzinçlio lu, 1985;Draber-Mo ko, 2004). However Wallman (2001) mentioned that SDF may be affected by cuticular shrinkage due to preservation and should be measured only in fresh specimens. Male (Figs 80,82).…”

Revision of the Nearctic Calliphora Robineau-Desvoidy (Diptera: Calliphoridae)

“…Calliphora Robineau-Desvoidy, 1830 (Diptera: Calliphoridae: Calliphorinae) is a cosmopolitan genus comprising about 76 species, with highest species richness in the Holarctic and Australasian Regions with about 26 and 36 species, respectively (Zumpt 1965;Norris 1973;Pont 1980;Schumann 1986;Schumann & Ozerov 1992;Norris 1994;Fan et al 1997;Wallman & Adams 1997;Rognes 1997Rognes , 1998Kurahashi & Selomo 1998;Wallman 2001;Rognes 2002;Verves 2002Verves , 2005Bharti 2011;Whitworth 2012;Kurahashi 2014Kurahashi , 2016. Most species of Calliphora are well known because of their medical and forensic importance (James 1947;Nuorteva 1963;Zumpt 1965;Greenberg 1971;Smith 1986;Greenberg & Kunich 2002;Graczyk et al 2005;Sawabe et al 2006;Förster et al 2007;Byrd & Castner 2010;Salvetti et al 2012).…”