Molecular tagging and candidate gene analysis of the high gamma-tocopherol trait in safflower (Carthamus tinctorius L.)

García-Moreno, M. J.; Fernández‐Martínez, José M.; Velasco, Leonardo; Pérez‐Vich, Begoña

doi:10.1007/s11032-010-9489-y

Cited by 15 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar case was reported in a safflower germplasm that accumulates predominantly γ‐tocopherol instead of α‐tocopherol (García‐Moreno et al. ). Both cases are examples of loss‐of‐function mutations.…”

Section: Discussionsupporting

confidence: 74%

Selection for contrasting tocopherol content and profile in Ethiopian mustard

et al. 2013

View full text Add to dashboard Cite

Tocopherols are compounds present in oilseeds with major effect on nutritional and technological properties of seed oils. Brassica spp. mainly contain varying concentrations of a-tocopherol, with high vitamin E value, and c-tocopherol, which contributes to oil stability. The objective of this research was to select for contrasting tocopherol content and profile in Ethiopian mustard (Brassica carinata A. Braun). A collection of 211 F 4:5 lines was evaluated for tocopherol content and profile, and divergent selection was conducted till the S 3:4 generation. Selection resulted in two lines with increased tocopherol content (244 and 243 mg/ kg seed as average of four environments), one line with reduced tocopherol content (117 mg/kg), two lines with reduced a/c-tocopherol ratio (0.22 and 0.28) and two lines with increased a/c-tocopherol ratio (3.74 and 3.34). Control line C-101 averaged 196 mg/kg with a/c-tocopherol ratio of 1.10. Reduced a/c-tocopherol ratio was controlled by recessive alleles at a single locus. Seed to plant correlation was high (r = 0.92), which suggests the feasibility of selecting at the single-seed level.

show abstract

Section: Discussionsupporting

confidence: 74%

Selection for contrasting tocopherol content and profile in Ethiopian mustard

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Identification of loci influencing agronomic traits facilitates marker-assisted breeding to increase crop productivity. In safflower, efforts to identify molecular markers linked to traits of agronomic value are limited (Hamdan et al, 2008 , 2012 ; Mayerhofer et al, 2010 ; García-Moreno et al, 2011 ; Pearl et al, 2014 ; Ebrahimi et al, 2017 ). In the present study, we performed association mapping and identified markers associated with eight traits of agronomic importance (seed oil content, oleic acid content, linoleic acid content, 100-seed weight, plant height, number of primary branches, days to 50% flowering and number of capitula per plant), many of which have been reported to influence crop yield (Patil, 1998 ).…”

Section: Discussionmentioning

confidence: 99%

“…These studies revealed high genetic differentiation among global safflower accessions and validated few “centers of similarity” of safflower that were identified based on morphological traits (Johnson et al, 2007 ; Chapman et al, 2010 ; Pearl and Burke, 2014 ; Kumar et al, 2015 ). Studies on development of linkage maps and tagging of agronomic traits using RAPDs, Restriction Fragment Length Polymorphism (RFLP), SSRs and SNP markers have been initiated in safflower (Hamdan et al, 2008 , 2012 ; Mayerhofer et al, 2010 ; García-Moreno et al, 2011 ; Pearl et al, 2014 ). Linkage analysis and/or QTL mapping is a conventional method of identifying genomic regions governing simple and/or complex traits but requires development of bi-parental mapping populations, which is a time consuming process.…”

Section: Introductionmentioning

confidence: 99%

Association Mapping for Important Agronomic Traits in Safflower (Carthamus tinctorius L.) Core Collection Using Microsatellite Markers

Ambreen

Kumar

et al. 2018

Front. Plant Sci.

View full text Add to dashboard Cite

Carthamus tinctorius L. (safflower) is an important oilseed crop producing seed oil rich in unsaturated fatty acids. Scarcity of identified marker-trait associations is a major limitation toward development of successful marker-assisted breeding programs in safflower. In the present study, a safflower panel (CartAP) comprising 124 accessions derived from two core collections was assayed for its suitability for association mapping. Genotyping of CartAP using microsatellite markers revealed significant genetic diversity indicated by Shannon information index (H = 0.7537) and Nei's expected heterozygosity (I = 0.4432). In Principal Coordinate Analysis, the CartAP accessions were distributed homogeneously in all quadrants indicating their diverse nature. Distance-based Neighbor Joining analysis did not delineate the CartAP accessions in consonance with their geographical origin. Bayesian analysis of population structure of CartAP demonstrated the unstructured nature of the association panel. Kinship analysis at population (Gij) and individual level (Fij) revealed absence of or weak relatedness between the CartAP accessions. The above parameters established the suitability of CartAP for association mapping. We performed association mapping using phenotypic data for eight traits of agronomic value (viz., seed oil content, oleic acid, linoleic acid, plant height, number of primary branches, number of capitula per plant, 100-seed weight and days to 50% flowering) available for two growing seasons (2011–2012 and 2012–2013) through General Linear Model and Mixed Linear Model. Our study identified ninety-six significant marker-trait associations (MTAs; P < 0.05) of which, several MTAs with correlation coefficient (R2) > 10% were consistently represented in both models and in both seasons for traits viz., oil content, oleic acid content, linoleic acid content and number of primary branches. Several MTAs with high R2-values were detected either in a majority or in some environments (models and/or seasons). Many MTAs were also common between traits (viz., oleic/linoleic acid content; plant height/days to 50% flowering; number of primary branches/number of capitula per plant) that showed positive or negative correlation in their phenotypic values. The marker-trait associations identified in this study will facilitate marker-assisted breeding and identification of genetic determinants of trait variability.

show abstract

“…The latter trait was found to be controlled by a single gene, allegedly a γ -TMT that converts γ -tocopherol into α -tocopherol. Loss of function of γ -TMT has resulted in nearly complete replacement of α -tocopherol by γ -tocopherol in sunflower [ 26 ] and safflower [ 27 ]. The opposite situation, that is, increased α -tocopherol and reduced γ -tocopherol relative concentrations, has been reported to occur due to higher activity of the γ -TMT promoter in soybean [ 28 ], to the presence of additional active copies of the γ -TMT gene in sunflower [ 29 ], and also to induction by overexpression of the γ -TMT gene in several species such as Arabidopsis , soybean, and Brassica juncea [ 13 , 30 – 32 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Reducedγ-Tocopherol Content in Ethiopian Mustard Seeds

García‐Navarro

Fernández‐Martínez

Pérez‐Vich

et al. 2016

The Scientific World Journal

View full text Add to dashboard Cite

Ethiopian mustard (Brassica carinata A. Braun) line BCT-6, with reduced γ-tocopherol content in the seeds, has been previously developed. The objective of this research was to conduct a genetic analysis of seed tocopherols in this line. BCT-6 was crossed with the conventional line C-101 and the F1, F2, and BC plant generations were analyzed. Generation mean analysis using individual scaling tests indicated that reduced γ-tocopherol content fitted an additive-dominant genetic model with predominance of additive effects and absence of epistatic interactions. This was confirmed through a joint scaling test and additional testing of the goodness of fit of the model. Conversely, epistatic interactions were identified for total tocopherol content. Estimation of the minimum number of genes suggested that both γ- and total tocopherol content may be controlled by two genes. A positive correlation between total tocopherol content and the proportion of γ-tocopherol was identified in the F2 generation. Additional research on the feasibility of developing germplasm with high tocopherol content and reduced concentration of γ-tocopherol is required.

show abstract

Molecular tagging and candidate gene analysis of the high gamma-tocopherol trait in safflower (Carthamus tinctorius L.)

Cited by 15 publications

References 31 publications

Selection for contrasting tocopherol content and profile in Ethiopian mustard

Selection for contrasting tocopherol content and profile in Ethiopian mustard

Association Mapping for Important Agronomic Traits in Safflower (Carthamus tinctorius L.) Core Collection Using Microsatellite Markers

Genetic Analysis of Reducedγ-Tocopherol Content in Ethiopian Mustard Seeds

Contact Info

Product

Resources

About