Molecular tailoring of farnesylation for plant drought tolerance and yield protection

Wang, Yan; Ying, Jifeng; Kuźma, Monika; Chalifoux, Maryse; Sample, Angela; McArthur, Charlene; Uchacz, Tina; Sarvas, Carlene; Wan, Jiangxin; Dennis, David T.; McCourt, Peter; Huang, Yunte

doi:10.1111/j.1365-313x.2005.02463.x

Cited by 223 publications

(131 citation statements)

References 35 publications

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“…Most reports that document enhancement of drought tolerance by means of overexpression of selected genes have been done with model systems, and there are only rare examples [e.g., ERA1 (34)] in which similar effects have been demonstrated through drought resistance testing in the field. With a long-term goal to improve drought resistance of rice, the stress-inducible SNAC1 gene was overexpressed in rice, and the transgenic plants were tested in the field for resistance to drought stress at the stage of anthesis.…”

Section: Discussionmentioning

confidence: 99%

Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice

Hu¹,

Dai²,

Yao

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,368

1,020

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Drought and salinity are major abiotic stresses to crop production. Here, we show that overexpression of stress responsive gene SNAC1 (STRESS-RESPONSIVE NAC 1) significantly enhances drought resistance in transgenic rice (22-34% higher seed setting than control) in the field under severe drought stress conditions at the reproductive stage while showing no phenotypic changes or yield penalty. The transgenic rice also shows significantly improved drought resistance and salt tolerance at the vegetative stage. Compared with WT, the transgenic rice are more sensitive to abscisic acid and lose water more slowly by closing more stomatal pores, yet display no significant difference in the rate of photosynthesis. SNAC1 is induced predominantly in guard cells by drought and encodes a NAM, ATAF, and CUC (NAC) transcription factor with transactivation activity. DNA chip analysis revealed that a large number of stress-related genes were up-regulated in the SNAC1-overexpressing rice plants. Our data suggest that SNAC1 holds promising utility in improving drought and salinity tolerance in rice.Oryza sativa ͉ abscisic acid ͉ stomata ͉ dehydration P oor water management, increased competition for limited water resources, and the uncertain threats associated with global warming all highlight the looming water crisis that threatens agricultural productivity worldwide. In China alone, the estimated annual loss of national economy from water shortage alone reaches Ͼ$25 billion (1). In addition to altered water management practices, the ability to enhance the tolerance of crops to drought and salinity stress, particularly at the most sensitive reproductive stage of growth, can have a potentially huge impact on productivity in the years to come.Plants can develop numerous physiological and biochemical strategies to cope with adverse conditions (2, 3). The major events of plant response to dehydration stresses are perception and transduction of the stress signals through signaling components, resulting in activation of a large number of stress-related genes and synthesis of diverse functional proteins that finally lead to various physiological and metabolic responses (4-6). Well characterized proteins involved in the protection of plant cells from dehydration stress damage include molecule chaperons, osmotic adjustment proteins (7), ion channels (8), transporters (9), and antioxidation or detoxification proteins (10). The expression of these functional proteins is largely regulated by specific transcription factors (4, 11).More than 30 families of transcription factors have been predicted for Arabidopsis (12). Members of DREB or CBF, MYB, bZIP, and zinc-finger families have been well characterized with roles in the regulation of plant defense and stress responses (4-6, 13, 14). Most of these transcription factors regulate their target gene expression through binding to the cognate cis-elements in the promoters of the stress-related genes. Two well characterized dehydration stress-related cis-elements bound by transcription factors are...

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Section: Discussionmentioning

confidence: 99%

Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice

Hu¹,

Dai²,

Yao

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,368

1,020

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“…Field trials suggested that with adequate water, transgenic canola produced the same amount of seed as the parental control. Under moderate drought stress conditions at flowering, the seed yield of transgenic plants was significantly higher than the control [4]. Overexpression of the RNAbinding domain of the flowering control locus A protein led to increase in plant size, organ size, cell size, plant productivity, and oil content in transgenic rape plants by down-regulating the cell-cycle-related cyclin-B2-1 gene, an activator of cyclin-dependent kinase 1 [5].…”

mentioning

confidence: 94%

“…Overexpression of wheat mitochondrial Mn superoxide dismutase (Mn SOD3.1) enhanced transgenic canola heat, drought and cold tolerance both in the field and under artificial stress conditions [3]. Transgenic B. napus carrying bsubunit of Arabidopsis farnesyltransferase (ERA1) antisense construct driven by a drought-inducible rd29A promoter were more resistant to seed abortion induced by water deficit during flowering [4]. Field trials suggested that with adequate water, transgenic canola produced the same amount of seed as the parental control.…”

mentioning

confidence: 99%

“…Biotechnological methods are successfully used for understanding plant abiotic stress tolerance [1] and for applying for rapeseed creation with different stress resistance [2][3][4][5]. Transgenic canola plants overexpressing a vacuolar Na + /H + antiport from Arabidopsis thaliana were able to grow, to flower, and to produce seeds in the presence of 200 mM NaCl.…”

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confidence: 99%

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cyp11A1 Canola plants under short time heat stress conditions

2014

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“…In waterdeficit condition, Trindade et al (2010) identified several conserved miRNAs having differential expression in M. truncatula plants as miR169 is down-regulated in roots, whereas miR398a/b and miR408 are strongly up-regulated in both shoots and roots. The RNAi-suppression of farnesyl transferase genes FTA or FTB, in canola leads to decreased stomatal conductance and thereby transpiration resulting in higher yields in a 3-year field trial (Wang 2005.…”

Section: Abiotic Stress Tolerancementioning

confidence: 99%