Molecular Targets of Herbicides and Fungicides─Are There Useful Overlaps for Fungicide Discovery?

Duke, Stephen O.; Pan, Zhiqiang; Bajsa-Hirschel, Joanna; Tamang, Prabin; Hammerschmidt, Raymond; Lorsbach, Beth A.; Sparks, Thomas C.

doi:10.1021/acs.jafc.3c07166

Cited by 5 publications

(3 citation statements)

References 199 publications

(343 reference statements)

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“…According to studies in the past years, more and more evidence has emerged to support comprehensive metabolism backgrounds underlying fungicide resistance. Such backgrounds include various ERG-encoding genes in fungal ergosterol biosynthesis pathways [36,41,42], acetyl-CoA carboxylase (ACCase)-encoding genes in the lipid and fatty acid oxidation path-ways [45][46][47], reactive oxygen species (ROS)-metabolizing enzyme-encoding genes in the cell wall maintenance and oxidative-stress-responsive processes [50,51], mitochondrial respiratory chain protein-encoding genes in the cellular energy metabolisms [53][54][55], ubiquitin-encoding genes in the post-translational modification processes [57][58][59], and protein kinase-encoding genes involved in mitogen-activated signal transductions [55,60,61]. In the present study, RNA-seq analysis revealed that Pdmfs2 knockout led to the down-regulation of genes involved in peroxisome (ko04146) and oxidative phosphorylation (ko00190) at no prochloraz treatment (Tables 1 and 2, and Figures 1 and 2).…”

Section: Discussionmentioning

confidence: 99%

“…These metabolisms and cellular stimuli processes in response to specific fungicide(s) have been studied, including ergosterol biosynthesis pathways, lipid and fatty acid oxidation pathways, cell wall maintenance, oxidative-stress-responsive processes, carbohydrate and amino acid metabolisms, cellular energy metabolisms, post-translational modification processes, and signal transduction pathways. All these pathways are highly dependent on many stress-responsive genes, including various ERG-encoding genes (such as erg1, erg3, erg11, erg24, erg25 and so on) [36,[40][41][42], acetyl-CoA carboxylase (ACCase)-encoding genes [43][44][45][46][47], reactive oxygen species (ROS)-metabolizing enzyme-encoding genes [48][49][50][51], mitochondrial respiratory chain protein-encoding genes [52][53][54][55], ubiquitin-encoding genes [56][57][58][59], and a series of protein kinase-encoding genes involved in mitogen-activated signal transductions [55,60,61]. As reported, MFS and ABC transporters played multiple roles in the transport of a diverse range of metabolic substrates and intermediates [62,63].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis of mfs2-Defective Penicillium digitatum Mutant to Reveal Importance of Pdmfs2 in Developing Fungal Prochloraz Resistance

Cuan,

Liu,

Zhou

et al. 2024

Microorganisms

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Demethylation inhibitors (DMIs), including prochloraz, are popular fungicides to control citrus postharvest pathogens such as Penicillium digitatum (green mold). However, many P. digitatum strains have developed prochloraz resistance, which decreases drug efficacy. Specific major facilitator superfamily (MFS) transporter gene mfs2, encoding drug-efflux pump protein MFS2, has been identified in P. digitatum strain F6 (PdF6) to confer fungal strain prochloraz resistance. However, except for the drug-efflux pump function of MFS2, other mechanisms relating to the Pdmfs2 are not fully clear. The present study reported a transcriptome investigation on the mfs2-defective P. digitatum strain. Comparing to the wild-type strain, the mfs2-defective strain showed 717 differentially expressed genes (DEGs) without prochloraz induction, and 1221 DEGs with prochloraz induction. The obtained DEGs included multiple isoforms of MFS transporter-encoding genes, ATP-binding cassette (ABC) transporter-encoding genes, and multidrug and toxic compound extrusion (MATE) family protein-encoding genes. Many of these putative drug-efflux pump protein-encoding genes had significantly lower transcript abundances in the mfs2-defective P. digitatum strain at prochloraz induction, as compared to the wild-type strain, including twenty-two MFS transporter-encoding genes (MFS1 to MFS22), two ABC transporter-encoding genes (ABC1 and ABC2), and three MATE protein-encoding genes (MATE1 to MATE3). The prochloraz induction on special drug-efflux pump protein genes in the wild-type strain was not observed in the mfs2-defective strain, including MFS21, MFS22, ABC2, MATE1, MATE2, and MATE3. On the other hand, the up-regulation of other drug-efflux pump protein genes in the mfs2-defective strain cannot recover the fungal prochloraz resistance, including MFS23, MFS26, MFS27, MFS31, MFS33, and ABC3 to ABC8. The functional enrichment of DEGs based on Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and euKaryotic Orthologous Groups (KOG) database resources suggested some essential contributors to the mfs2-relating prochloraz resistance, including ribosome biosynthesis-related genes, oxidative phosphorylation genes, steroid biosynthesis-related genes, fatty acid and lipid metabolism-related genes, and carbon- and nitrogen-metabolism-related genes. The results indicated that the MFS2 transporter might be involved in the regulation of multiple drug-efflux pump protein gene expressions and multiple metabolism-related gene expressions, thus playing an important role in developing P. digitatum prochloraz resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of mfs2-Defective Penicillium digitatum Mutant to Reveal Importance of Pdmfs2 in Developing Fungal Prochloraz Resistance

Cuan,

Liu,

Zhou

et al. 2024

Microorganisms

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“…Among the newly published articles, Duke et al explored the overlap of herbicide and fungicide molecular targets . Doherty et al examined worker exposure in residential and golf course environments, and Sun et al shared their cloning, expression, and functional characterization of flavonoid-di- O -glycosyltransferases in maize …”

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confidence: 99%

Celebrating Fifty Years of AGRO and a Fruitful Partnership with JAFC

McConnell,

Sparks,

Van Emon

et al. 2024

J. Agric. Food Chem.

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Phytotoxic and Antifungal Activity of (−)‐Penienone Produced by Penicillium palitans (Ascomycota) Isolated from Deep Sea Sediments in the Southern Ocean, Maritime Antarctica

Barreto,

Lowell Cantrell,

Karla da Silva

et al. 2024

Chemistry & Biodiversity

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Metabolites isolated from Penicillium palitans, obtained from deep sea sediments in the maritime Antarctica, were investigated for phytotoxic and antifungal activities. The fungus was submitted to solid state fermentation, and its crude extract was produced. Chromatographic separations of the P. palitans methylene chloride crude extract led to the isolation of two analogous compounds: (−)‐penienone (1) and (−)‐palitantin (2). The phytotoxic activity of these compounds was assessed against seeds of Lactuca sativa and Agrostis stolonifera. Both compounds demonstrated activity exclusively in the monocotyledonous model, A. stolonifera. At a concentration of 1 mg mL−1, (−)‐penienone caused 100 % inhibition of seed germination, while (−)‐palitantin had only moderate activity on germination and growth of seedlings at this concentration after 7 days of treatment. Against Lemna paucicostata, at 57 μM, (−)‐penienone inhibited growth by 50 % (IC50). (−)‐Palitantin was much less active. When evaluated for their antifungal activity against Colletotrichum fragariae, only (−)‐penienone had an IC50 value of 0.3 μM. The strain P. palitans was shown to be a promising source of phytotoxic and antifungal compounds, suggesting that extremophilic fungi, such as wild Penicillium strains from Antarctica, have the potential to yield novel prototype molecules for new pesticide development.

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Molecular Targets of Herbicides and Fungicides─Are There Useful Overlaps for Fungicide Discovery?

Cited by 5 publications

References 199 publications

Transcriptome Analysis of mfs2-Defective Penicillium digitatum Mutant to Reveal Importance of Pdmfs2 in Developing Fungal Prochloraz Resistance

Transcriptome Analysis of mfs2-Defective Penicillium digitatum Mutant to Reveal Importance of Pdmfs2 in Developing Fungal Prochloraz Resistance

Celebrating Fifty Years of AGRO and a Fruitful Partnership with JAFC

Phytotoxic and Antifungal Activity of (−)‐Penienone Produced by Penicillium palitans (Ascomycota) Isolated from Deep Sea Sediments in the Southern Ocean, Maritime Antarctica

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