Molecular Techniques: Applications in Epidemiologic Studies

Harris, Natalie; Thoen, Charles O.; Steele, James H.; Gilsdorf, Michael J.

doi:10.1002/9780470344538.ch7

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…Neste caso, os isolados de BAAR são submetidos à técnica TB Multiplex PCR (WILTON; COUSINS, 1992) e aqueles classificados como pertencentes ao complexo M. tuberculosis, são submetidos ao RD Multiplex PCR (RD9 e RD12) (WARREN et al, 2006), o qual discriminará o M. bovis dos outros membros do complexo M. tuberculosis. Além da identificação da espécie da micobactéria, os métodos moleculares permitem discriminar isolados de uma mesma espécie, como o Spoligotyping e o MIRU (Mycobacterial Interpersed Repetitive Units), que discriminam isolados de M. bovis(KAMERBEEK et al, 1997;SUPPLY et al, 2000) e o RFLP (Restriction Fragment Lenght Polymorphism), que discrimina isolados de M. tuberculosis (VAN EMBDEN et al, 1993;HARRIS, 2006).O Spoligotyping requer de um a dois dias e é utilizado sobretudo para discriminação de isolados de M. bovis. Além disso, em certa medida, permite também a diferenciação entre M. bovis e M. tuberculosis.…”

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Tuberculose em primatas

Valvassoura

Neto²

2023

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A Tuberculose é um dos maiores problemas sanitários dos animais selvagens mantidos em cativeiro para os mais variados propósitos. Grande parte das ocorrências envolve a transmissão zoonótica e os primatas não humanos são os animais mais frequentemente afetados. Em função das complexidades para o seu controle, determina grandes perdas econômicas e a estigmatização da instituição que a reporta. Enquanto nos países da América do Norte e Europa é tratada como prioridade, havendo estratégias definidas por legislação, no Brasil, existe quase completa omissão em relação à doença, o que resulta em individualismo no trato do problema por parte das instituições mantenedoras de animais cativos. Assim, este livro trata desse assunto sob a ótica da medicina veterinária preventiva, com natural ênfase nas populações animais. Aborda desde aspectos históricos até sistemas de vigilância, passando por detalhes da etiologia, epidemiologia, patogenia, sinais clínicos, diagnóstico e legislação brasileira, visando chamar atenção para o tema e inspirar iniciativas para o bom encaminhamento da questão no nosso meio.

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Tuberculose em primatas

Valvassoura

Neto²

2023

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“…Hence, comparing the genetic profiles between animal and human isolates of M. bovis enables to detect the source(s) of infection and the route(s) of transmission, and to predict the future picture of disease progression. The use of this technique is spreading in most areas of research, academic centers and TB specialized hospitals [88,89]. Therefore, using the molecular biology application, African countries where livestock and wildlife are living in close association with human, will help in detecting the source of infection as well as transmission dynamicity.…”

Section: Transmission Status Of Mycobacterium Bovismentioning

confidence: 99%

Molecular Characterization of Mycobacterium bovis and its Significance: Role for Control of Zoonotic Tuberculosis in Africa

Getahun¹

2017

J Med Diagn Meth

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Mycobacterium bovis is the main causal agent of bovine tuberculosis (BTB) that causes zoonotic tuberculosis (TB) in humans, even though M. caprae; contribute to a lesser extent, which is mostly acquired from domestic animals and their products, in which cattle are the major reservoir. This review paper discussed the importance of zoonotic tuberculosis in Africa and highlighted the uses of molecular methods in diagnosis, control and prevention of zoonotic tuberculosis. Despite the fact, there are various diagnostic methods available nowadays; the molecular techniques are proving more rapid and plausible sensitivity and specificity results that increase the diagnosis precision. In addition, molecular diagnostic methods used to indicate the epidemiological status of different strain distribution with regard to cluster distribution and reactivation of dormant or treated cases of tuberculosis indicating the drug resistance situation of the strain. Hence, applying molecular techniques for diagnosis of zoonotic tuberculosis in developing countries will help in decreasing the incidence, prevalence, mortality, morbidity and loss of economic due to carcass condemnation, milk drop and livestock trade banning in the continent. Advanced molecular methods truly play a key role in prevention and control program of tuberculosis in general and bovine tuberculosis in specific, which leads to the cooperation of various disciplines. Introducing cost effective and easy molecular diagnostic methods is recommended, implementing molecular diagnostic methods in research areas and intensive training of personnel in the use of equipment.

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“…M. bovis , the agent of bovine tuberculosis, is a slow -growing nonphotochromogenic organism that also causes disease in other domestic and wild animals, and has been reported in humans in several countries (Thoen et al 2006 ; Thoen et al 2009 ). Biochemical tests are available for differentiating bacteria of the M. tuberculosis complex, but molecular techniques are now widely used in reference laboratories around the globe (Harris 2006 ;Thoen et al 2009 ). Mycobacterium leprae , the cause of leprosy (also known as Hansen ' s disease), is a chronic granulomatous disease.…”

Section: Characteristics and Sources Of The Organismsmentioning

confidence: 99%