Molecular techniques for sex identification of captive birds

Purwaningrum, Medania; Nugroho, Herjuno Ari; Asvan, Machmud; Karyanti, Karyanti; Alviyanto, Bertha; Kusuma, Randy; Haryanto, Aris

doi:10.14202/vetworld.2019.1506-1513

Cited by 32 publications

(29 citation statements)

References 45 publications

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“…AF11). This result was in line with other researchers, who carried out the molecular bird sexing on some species of captive birds belong to the families of Psittacidae, Cacatuidae, Psittaculidae, Psittrichasiidae, Pelecanidae, Anatidae, Phoenicopteridae, Spheniscidae, Threskiornithidae and Musophagidae [13].…”

Section: Resultssupporting

confidence: 92%

Molecular Bird Sexing on Fischeri Lovebird (Agapornis fischeri) by Using Polymerase Chain Reaction

et al. 2020

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Fischeri Lovebird (Agapornis fischeri) found originally in Africa which has spread to many countries. In Indonesia, Fischeri Lovebird is popular as a pet animal. This lovebird is a monomorphic bird, so it is difficult to differentiate morphologically between male and female birds. In general, a male lovebird has ZZ homozygotes, whereas females' lovebird has ZW heterozygous of their sex chromosome. These sex chromosomes set used as study targets for molecular bird sexing of many species of birds because this method is effective and simple to perform. This method targeted to amplify the Chromodomain Helicase DNA-binding (CHD) gene, which found into the sex chromosome of male and female birds. The objective of this study was to rapid molecular bird sexing of Fischeri Lovebird by using PCR methods. Research samples were collected from feather calamus of A. fischeri. The total sample was 11 feathers from A. fischeri. which were collected three to six feathers for each lovebird. Then the research was followed by DNA extraction from calamus feathers, DNA amplification by PCR and agarose gel electrophoresis of PCR products and visualization of PCR predicts by UV-Transilluminator in darkroom. It concluded that PCR amplification using NP, MP and P2 primers produced double DNA bands in size of 400 bp on Z chromosome and bp on W chromosome for female Fischeri Lovebird, whereas for male Fischeri Lovebird only produced a single DNA band in size of 400 bp on Z chromosome. From eleven samples of Fischeri Lovebird showed a total of five females and six male Fischeri Lovebirds.

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Section: Resultssupporting

confidence: 92%

Molecular Bird Sexing on Fischeri Lovebird (Agapornis fischeri) by Using Polymerase Chain Reaction

et al. 2020

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“…Each sample was subjected to three repetitions of PCR for consistency. The PCR mix used was GoTaq® Green Master Mix, with the composition and temperature cycle referring to [10] with modification. The PCR compositions used were DNA template (20 ng/µl), forward and reverse primer (@ 10 pmol), MgCl2 (25mM), 1X GoTaq® Green Master 2X PCR buffer Mix, and ddH2O to total volume of 25 µl.…”

Section: Dna Amplificationmentioning

confidence: 99%

“…Sex determination was carried out based on the results of visualization of DNA amplification on agarose gel. The presence of a single DNA band indicates that the sample is male (ZZ chromosome) and the presence of a double DNA band (ZW chromosome) indicates that the sample is female [10].…”

Section: Introductionmentioning

confidence: 99%

DNA sexing for gender determination of Changeable Hawk-Eagle (Nisaetus cirrhatus, Gmelin, 1788)

2021

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The Changeable hawk-eagle (Brontok eagle) is a protected bird species. It is one of the most frequently hunted and traded birds in Indonesia. The processes of being traded changes this bird natural behaviour. Therefore, a rehabilitation effort to return the eagle’s behaviour to conform to its natural habits is needed. The ultimate goal of rehabilitation is to release the changeable hawk-eagle back into its natural habitat. In conservation and breeding programs, efforts to determine the sex of eagles to be released are very important to help increase the population of changeable hawk-eagles in their habitat by looking at the sex ratio. At the present, sex determination at the Kamojang Conservation Eagle Center (Pusat Konservasi Elang Kamojang or PKEK) uses the morphometric method. This research used the DNA sexing method with primers 2550F and 2718R to determine the sex of Changeable hawk-eagles in PKEK by extracting DNA from blood samples of 30 eagles. Comparison of DNA sexing results and morphometric data showed differences. This proves that DNA sexing, is suitable in determining changeable hawk-eagles’ sex.

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“…Sexing on young birds could still be performed using chromodomain helicase DNA (CHD) binding detection with PCR (Çakmak et al, 2017). Blood, feathers, tissue, buccal, and cloacal swab samples could be used in this method (Doosti et al, 2009;Çakmak et al, 2017;Purwaningrum et al, 2019). Eggshell corticosterone levels seem to be high in males than females in Japanese quails (Qasimi et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Some modern methods that have developed in current years are laparoscopy and laparotomy to observe the gonads directly (Vucicevic et al, 2013;Divers, 2015), polymerase chain reaction (PCR) for gene study (Morinha et al, 2012), steroid sexing to observe the sexual hormone levels Biogenesis 90 from eggshell and fecal samples (Adkins-Regan, 2007;Cerit & Avanus, 2007;Abdollahi et al, 2018;Qasimi et al, 2018;Purwaningrum et al, 2019); genom evaluation and in silico approach (Ishijima et al, 2014;Caetano-Anolles et al, 2015;Rallabandi et al, 2019). DNA isolation could be collected from feather, blood, feces, buccal and cloacal swabs (Çakmak et al, 2017;Abdollahi et al, 2018;Asawakarn et al, 2018;Purwaningrum et al, 2019). Unfortunately, most modern methods are costly and rarely applied among the Indonesia bird keeping community.…”

Section: Introductionmentioning

confidence: 99%

Beak Line and Eye Alignment as Phenotypic Sexing for Domestic Canaries (Serinus canaria)

Akrom¹,

Indarjulianto

Yanuartono³

et al. 2020

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Phenotypic sexing of birds is a common practice among the songbird-keeping community, yet it is based on non-reputable information. This study aims to determine the sex of canaries (Serinus canaria) based on the alignment of the eye with the beak line. A total of 26 samples, consisting of six samples of one-month-old canaries (three males and three females based on PCR examination), 20 samples of sixmonth-old canaries (ten proven breeding pairs) were used in this study. The birds' heads were photographed from the sides, and then the positions of the eyes were compared with the shadow alignment of the beak. The results provided that five young birds and ten adult birds have a beak line alignment under the eye, while ten adult birds and one young bird have a beak line alignment across the eye. The accuracy of sexing using this method was 100% for both female and male adult canaries. However, this result could not be applied to sample that are not yet sexually mature, as two young male birds were found to have beak alignment below the eye. It can be concluded that sexing adult canaries can be performed by observing the eye and beak line's alignment.

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Molecular techniques for sex identification of captive birds

Cited by 32 publications

References 45 publications

Molecular Bird Sexing on Fischeri Lovebird (Agapornis fischeri) by Using Polymerase Chain Reaction

Molecular Bird Sexing on Fischeri Lovebird (Agapornis fischeri) by Using Polymerase Chain Reaction

DNA sexing for gender determination of Changeable Hawk-Eagle (Nisaetus cirrhatus, Gmelin, 1788)

Beak Line and Eye Alignment as Phenotypic Sexing for Domestic Canaries (Serinus canaria)

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