Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management

Jenkins, Cheryl; Chapman, Toni A.; Micallef, Jessica L.; Reynolds, Olivia L.

doi:10.3390/insects3030763

Cited by 32 publications

(34 citation statements)

References 122 publications

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“…However, B. carambolae and B. papayae were determined to infest several crops species from different families, for example, Manilkara zapota, Musa acuminate, Carica papaya, Psidium guajava, Artocarpus altilis, Mangifera indica and Averrhoa carambola. Therefore, comprehensive and precise knowledge regarding the host plant and pest interactions is highly desirable in the effective suppression and control of the fruit fly populations (Jenkins et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Molecular clock analysis on fiveBactroceraspecies flies (Diptera: Tephritidae) based on combination ofCOIandNADHsequences

et al. 2015

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This paper marks the first attempt at using the molecular clock analysis on five Malaysian Bactrocera species based on combined data of COI and ND1 to test the divergence times of species that infested 11 varieties of fruits and vegetables. A divergence tree was constructed to estimate the cryptic species among the five closely related Bactrocera species viz. B. (Bactrocera) and B. (Zeugodacus). Both subgenera evolved and diverged approximately 21.5933 million years ago (MYA). The particularly complex taxonomical status of the sister-species B. papayae and B. carambolae has been resolved using the divergence tree; the two separated from the others approximately 2.743 MYA, while B. papayae and B. carambolae distinctly diverged between 1.9619 and 2.2884 MYA. Additionally, Bactrocera dorsalis showed divergence at 4.1596 MYA and was distinctly separate from the B. papayae. Interestingly, the molecular clock results are congruent with the phylogenetic tree that was analysed using Maximum Parsimony and Bayesian Inference. Further, the taxonomical status and host utilisation of the five Bactrocera species were also briefly discussed, which indicated that B. cucurbitae (subgenus Zeugodacus) was infesting Cucurbitaceae and B. latifrons was infesting Solanaceaea, while B. dorsalis, B. carambolae and B. papaya were infesting a wide range of fruit species. Key message Divergence time of five Malaysian Bactroceran species is reported for the first time and found congruent with the results of phylogeny based on combined data of COI and ND1 markers using MP and Bayesian analyses. This finding could prove very crucial for resolving the most crucial taxonomic conflicts in Bactrocera classification, which is highly important initially in species identification for integrated pest management (IPM) programme.

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Section: Discussionmentioning

confidence: 99%

Molecular clock analysis on fiveBactroceraspecies flies (Diptera: Tephritidae) based on combination ofCOIandNADHsequences

et al. 2015

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“…Armstrong and Ball (2005) also demonstrated the suitability of DNA barcodes in providing a practical, cost-effective, and ßexible framework for the accurate diagnostic of morphologically indistinct intercepted specimens. Increasingly, molecular diagnostic tests are accepted as an essential component of detection and identiÞcation systems of exotic invasive species (Jenkins et al 2012).…”

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confidence: 99%

Detection and Genetic Diversity of a Heliothine Invader (Lepidoptera: Noctuidae) From North and Northeast of Brazil

et al. 2014

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The cotton bollworm, Helicoverpa armigera (Hübner), was recently introduced in Brazil. During the 2012-2013 harvest, producers reported reduced yields up to 35% on major crops. The economic losses reached US$ 1 billion only in western Bahia, triggering a phytosanitary crisis. The deficiencies in existing taxonomic keys to deal with the morphologically indistinct larvae of H. armigera and the native Helicoverpa zea (Boddie) constrained the detection of new incursions of this heliothine invader. This study explored the identity of heliothine larvae that were found infesting soybean- and corn-growing areas from Roraima state, northern Brazil, through sequences of the mitochondrial cytochrome c oxidase subunit I gene. The inter- and intraspecies sequence variations of DNA barcodes in H. armigera and H. zea were analyzed. The genetic diversity and population structure of the specimens from Roraima and two populations from Piauí and Bahia states, northeastern Brazil, were assessed by adding the cytochrome c oxidase subunit II gene to the analysis. Owing to the lack of studies on genetic introgression for the two species, the suitability of using three different nuclear genes to distinguish the two species was also investigated. The results showed strong evidence that the heliothine larvae from north and northeast of Brazil are conspecific with H. armigera, suggesting that this invasive moth has already crossed the Amazon basin. Surveys in the north of South America should start as soon as possible to monitor the entry or spread of this moth in the Caribbean, Central America, and the United States.

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“…(Diptera: Tephritidae) by using mtDNAs (12S and 16S rDNA and NADH-dehydrogenase subunit 6). PCR-RFLP-based molecular markers have also been used to discriminate other cryptic Diptera species, including morphologically indistinguishable parasitoids (Jenkins et al 2012). In this study, a molecular identiÞca-tion technique involving PCR-RFLP was developed to distinguish two indigenous sand ßy species in Kentucky (and much of the eastern United States), L. shannoni and Lutzomyia vexator Coquillett.…”

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Molecular Identification of Sand Flies (Diptera: Psychodidae) in Eastern North America by Using PCR-RFLP

et al. 2013

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Sand flies (Diptera: Psychodidae) are small blood-feeding dipterans that are primary vectors of numerous human and livestock pathogens. Effective surveillance programs with accurate identification tools are critical in development and implementation of modern integrated pest management programs. Although morphological keys are available for North American species, identification can still be challenging owing to the nature of sample preparation and incompatibility with molecular or biochemical-based pathology assays. Further, the potential for introduction of Old World or other exotic species is not accounted for by current keys. Herein, we present the development and validation of a restriction fragment-length polymorphism-based molecular identification method. Specifically, cytochrome c oxidase subunit I, a mitochondrial DNA marker, was used to distinguish two species of adult sand flies indigenous to eastern North America with two exotic species not yet known to occur in the United States.

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Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management

Cited by 32 publications

References 122 publications

Molecular clock analysis on fiveBactroceraspecies flies (Diptera: Tephritidae) based on combination ofCOIandNADHsequences

Molecular clock analysis on fiveBactroceraspecies flies (Diptera: Tephritidae) based on combination ofCOIandNADHsequences

Detection and Genetic Diversity of a Heliothine Invader (Lepidoptera: Noctuidae) From North and Northeast of Brazil

Molecular Identification of Sand Flies (Diptera: Psychodidae) in Eastern North America by Using PCR-RFLP

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