Molecular techniques in wastewater: Understanding microbial communities, detecting pathogens, and real-time process control

Gilbride, Kimberley; Lee, D.-Y.; Beaudette, Lee A.

doi:10.1016/j.mimet.2006.02.016

Cited by 161 publications

(90 citation statements)

References 157 publications

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“…Different multiplex PCR assays have been developed to detect waterborne bacterial pathogens (including Salmonella spp., Shigella spp., Aeromonas spp., Vibrio spp. ), waterborne viral pathogens (e.g., Norwalk virus, astroviruses, and enteroviruses), or multiple microbial pathogens (such as V. cholera, calicivirus, and Aureococcus anophagefferens) [36][37][38][39].…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Recommended advanced techniques for waterborne pathogen detection in developing countries

Alhamlan

Al‐Qahtani

Al-Ahdal

2015

J Infect Dev Ctries

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The effect of human activities on water resources has expanded dramatically during the past few decades, leading to the spread of waterborne microbial pathogens. The total global health impact of human infectious diseases associated with pathogenic microorganisms from landbased wastewater pollution was estimated to be approximately three million disability-adjusted life years (DALY), with an estimated economic loss of nearly 12 billion US dollars per year. Although clean water is essential for healthy living, it is not equally granted to all humans. Indeed, people who live in developing countries are challenged every day by an inadequate supply of clean water. Polluted water can lead to health crises that in turn spread waterborne pathogens. Taking measures to assess the water quality can prevent these potential risks. Thus, a pressing need has emerged in developing countries for comprehensive and accurate assessments of water quality. This review presents current and emerging advanced techniques for assessing water quality that can be adopted by authorities in developing countries.

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Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Recommended advanced techniques for waterborne pathogen detection in developing countries

Alhamlan

Al‐Qahtani

Al-Ahdal

2015

J Infect Dev Ctries

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“…Despite the environmental importance of these processes, the knowledge of the microbial communities within biological wastewater treatment is incomplete, primarily because of a lack of suitable tools for their analysis [3]. Culture-dependent methods have some limitations because they do not permit the retrieval of most of the bacteria present in complex communities and hence give a poor understanding of microbial community structure [4,5]. The development of conventional molecular techniques in the last two decades, such as fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE), clone libraries, etc., have provided new insights into microbial community structure.…”

Section: Introductionmentioning

confidence: 99%

MiSeq Sequencing Analysis of Bacterial Community Structures in Wastewater Treatment Plants

Wen

Jin

Wang

et al. 2015

Pol. J. Environ. Stud.

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The bacterial communities of activated sludge from nine wastewater treatment plants (WWTPs) located in three cities in China were examined using high-throughput MiSeq sequencing. The results showed that there were 57 genera of bacterial populations commonly shared by all nine systems, including Ferruginibacter, Dechloromonas, Zoogloea, Gp4, Gp6, etc., indicating that there was a core microbial community in the microbial populations of WWTPs at different geographic locations. Canonical correspondence analysis (CCA) results indicated that the bacterial community variance correlated most strongly with water temperature, dissolved oxygen (DO), concentrations of chemical oxygen demand (COD), and solids retention time (SRT). Variance partitioning analyses suggested that wastewater characteristics had the greatest contribution to the bacterial community variance, explaining 21% of the variance of bacterial communities independently, followed by operational parameters (17%), and geographic location (11%). This study provided insights into the diversity and bacterial community structures in geographically distributed WWTPs.

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“…These include molecular methods to establish the library of DNA profiles for fecal bacteria, protozoan and viruses. The most widely applied methods for molecular fingerprinting are denaturing gradient gel electrophoresis, temperature gradient gel electrophoresis and terminal restriction fragment length polymorphism analysis [23][24][25]. Selection of the appropriate MST markers would provide rapid detection of wastewater contamination directed to the specific source.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Use of Chemical Markers for Tracing Wastewater Contamination in Aquatic Environment: A Review

Lim

Ong

2017

Water

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There has been increasing research focus on the detection and occurrence of wastewater contamination in aquatic environment. Wastewater treatment plants receive effluents containing various chemical pollutants. These chemicals may not be fully removed during treatment and could potentially enter the receiving water bodies. Detection of these chemical pollutants and source identification could be a challenging research task due to the diversified chemical and functional groups, concentration levels and fate and transportation mechanisms of these pollutants in the environment. Chemical markers such as pharmaceuticals and personal care products, artificial sweeteners, fluorescent whitening agents, sterols and stanols, and nitrate and nitrogen isotopics have been widely used by most research as markers. These markers served as indicators of wastewater contamination to the receiving bodies due to their frequent usage, resistance to biodegradability and, more importantly, anthropogenic origin. These markers are commonly used in combination to identify the contaminant source of different origins. This article discusses the main chemical markers that are used to identify wastewater contamination in receiving bodies, the current trends, and approach to select suitable chemical markers.

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Molecular techniques in wastewater: Understanding microbial communities, detecting pathogens, and real-time process control

Cited by 161 publications

References 157 publications

Recommended advanced techniques for waterborne pathogen detection in developing countries

Recommended advanced techniques for waterborne pathogen detection in developing countries

MiSeq Sequencing Analysis of Bacterial Community Structures in Wastewater Treatment Plants

Recent Advances in the Use of Chemical Markers for Tracing Wastewater Contamination in Aquatic Environment: A Review

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