Molecular toxicity and defense mechanisms induced by silver nanoparticles in Drosophila melanogaster

Wang, Zhidi; Zhang, Liying; Wang, Xing

doi:10.1016/j.jes.2021.12.027

Cited by 14 publications

(6 citation statements)

References 77 publications

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“…For example, at a concentration of 0.1%, the emergence rates of T. castaneum treated with Bt-Ag 2 O NPs, Ag 2 O NPs, and Bt were 2.22%, 6.83%, and 45.74%, respectively. These results were similar to those of previous studies; for example, Ag NPs significantly decreased Drosophila melanogaster fecundity, larval weight, rate of pupation, and emergence in a dose-dependent manner (Wang et al 2023 ). Bt-Ag 2 O NPs showed higher pesticidal activity than Ag 2 O NPs and Bt, revealing the effect of bioactive substances from Bt against insects.…”

Section: Discussionsupporting

confidence: 92%

“…Bt-Ag 2 O NPs showed higher pesticidal activity than Ag 2 O NPs and Bt, revealing the effect of bioactive substances from Bt against insects. Some insect pests display resistance to Bt , while metallic nanoparticles synthesized using Bt can strengthen its pesticidal activities, which can cause greater inhibition of α-amylase; as a result, the development of organisms or cells is slower and the body sizes are smaller (Wang et al 2023 ). The details underlying the insect response to Ag/Ag 2 O NPs still need to be further studied in the future.…”

Section: Discussionmentioning

confidence: 99%

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Biosynthesis of Bt-Ag2O nanoparticles using Bacillus thuringiensis and their pesticidal and antimicrobial activities

Ge,

Hu,

Cui

et al. 2024

Appl Microbiol Biotechnol

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Nanosilver oxide exhibits strong antibacterial and photocatalytic properties and has shown great application potential in food packaging, biochemical fields, and other fields involving diseases and pest control. In this study, Ag2O nanoparticles were synthesized using Bacillus thuringiensis (Bt-Ag2O NPs). The physicochemical characteristics of the Bt-Ag2O NPs were analyzed by UV‒vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), inductively coupled plasma emission spectrometry (ICP), high-resolution transmission electron microscopy (HR-TEM), and zeta potential. The phis-chemical characterization revealed that the Bt-Ag2O NPs are in spherical shape with the small particle size (18.24 nm), high crystallinity, well dispersity, and stability. The biopesticidal and antifungal effects of Bt-Ag2O NPs were tested against Tribolium castaneum, Aspergillus flavus, and Penicillium chrysogenum. The survival, growth, and reproduction of tested pests and molds were significantly inhibited by Bt-Ag2O NPs in a dose-dependent manner. Bt-Ag2O NPs showed higher pesticidal activities against T. castaneum than Bt and commercial Ag2O NPs. The LC50 values of Bt, Ag2O NPs, and Bt-Ag2O NPs were 0.139%, 0.072%, and 0.06% on day 14, respectively. The Bt-Ag2O NPs also showed well antifungal activities against A. flavus and P. chrysogenum, while it resulted a small inhibition zone than commercial Ag2O NPs did. In addition, A. flavus showed much more sensitive to Bt-Ag2O NP treatments, compared to P. chrysogenum. Our results revealed that Bt-Ag2O NPs synthesized using B. thuringiensis could act as pesticides and antifungal agents in stored-product fields. Key points • Bt-Ag2O NPs could be synthesized using Bacillus thuringiensis (Bt). • The NPs showed a high degree of crystallinity, spherical shape, and small particle size. • The NPs also showed excellent insecticidal and antifungal activity.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Biosynthesis of Bt-Ag2O nanoparticles using Bacillus thuringiensis and their pesticidal and antimicrobial activities

Ge,

Hu,

Cui

et al. 2024

Appl Microbiol Biotechnol

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“…ROS was considered as a major cause of nanoparticle-induced toxicity in organisms [ 69 – 74 ]. But previous studies were focusing on the damages caused by ROS intracellularly [ 75 – 80 ]. Recently ROS were discovered to control cell differentiation and cellular immune response in a cell-non-autonomous fashion [ 81 , 82 ].…”

Section: Discussionmentioning

confidence: 99%

Chronic exposure to the star polycation (SPc) nanocarrier in the larval stage adversely impairs life history traits in Drosophila melanogaster

Yan

Guo

et al. 2022

J Nanobiotechnol

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Background Nanomaterials are widely used as pesticide adjuvants to increase pesticide efficiency and minimize environmental pollution. But it is increasingly recognized that nanocarrier is a double-edged sword, as nanoparticles are emerging as new environmental pollutants. This study aimed to determine the biotoxicity of a widely applied star polycation (SPc) nanocarrier using Drosophila melanogaster, the fruit fly, as an in vivo model. Results The lethal concentration 50 (LC50) value of SPc was identified as 2.14 g/L toward third-instar larvae and 26.33 g/L for adults. Chronic exposure to a sub lethal concentration of SPc (1 g/L) in the larval stage showed long-lasting adverse effects on key life history traits. Exposure to SPc at larval stage adversely impacted the lifespan, fertility, climbing ability as well as stresses resistance of emerged adults. RNA-sequencing analysis found that SPc resulted in aberrant expression of genes involved in metabolism, innate immunity, stress response and hormone production in the larvae. Orally administrated SPc nanoparticles were mainly accumulated in intestine cells, while systemic responses were observed. Conclusions These findings indicate that SPc nanoparticles are hazardous to fruit flies at multiple levels, which could help us to develop guidelines for further large-scale application.

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“…At present, many nanomaterials have been successfully used to enhance the specificity and efficiency of PCR, such as Au NPs [5], QDs [6], CNTs [7], graphene oxide (GO) and reduced GO (rGO) [8], partial metal oxidation materials [9,10] (e.g., titanium dioxide, zinc oxide) and other composite materials like macromolecule polymer doped with Au NPs [11], amino-modified semiconductor magnetic NPs [12], and so on. Nanomaterials are composed of particles with at least one external dimension less than 100 nm, and they have been widely used in electronics, aerospace, military, chemical, biomedical, and healthcare products [13]. In PCR, nanomaterials are added to the reaction system that mainly contains primers, enzymes, and templates, due to the characteristics of nanomaterials and their role in PCR.…”

Section: Polymerase Chain Reaction (Pcr) Technology First Proposed By...mentioning

confidence: 99%

Application of Nanomaterials to Enhance Polymerase Chain Reaction

et al. 2022

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Polymerase Chain Reaction (PCR) is one of the most common technologies used to produce millions of copies of targeted nucleic acid in vitro and has become an indispensable technique in molecular biology. However, it suffers from low efficiency and specificity problems, false positive results, and so on. Although many conditions can be optimized to increase PCR yield, such as the magnesium ion concentration, the DNA polymerases, the number of cycles, and so on, they are not all-purpose and the optimization can be case dependent. Nano-sized materials offer a possible solution to improve both the quality and productivity of PCR. In the last two decades, nanoparticles (NPs) have attracted significant attention and gradually penetrated the field of life sciences because of their unique chemical and physical properties, such as their large surface area and small size effect, which have greatly promoted developments in life science and technology. Additionally, PCR technology assisted by NPs (NanoPCR) such as gold NPs (Au NPs), quantum dots (QDs), and carbon nanotubes (CNTs), etc., have been developed to significantly improve the specificity, efficiency, and sensitivity of PCR and to accelerate the PCR reaction process. This review discusses the roles of different types of NPs used to enhance PCR and summarizes their possible mechanisms.

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Molecular toxicity and defense mechanisms induced by silver nanoparticles in Drosophila melanogaster

Cited by 14 publications

References 77 publications

Biosynthesis of Bt-Ag2O nanoparticles using Bacillus thuringiensis and their pesticidal and antimicrobial activities

Biosynthesis of Bt-Ag2O nanoparticles using Bacillus thuringiensis and their pesticidal and antimicrobial activities

Chronic exposure to the star polycation (SPc) nanocarrier in the larval stage adversely impairs life history traits in Drosophila melanogaster

Application of Nanomaterials to Enhance Polymerase Chain Reaction

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