Molecular Typing and Antimicrobial Susceptibility of Vancomycin-Resistant<i>Enterococcus faecium</i>in Brazil

Cereda, Rosângela Ferraz; Gales, Ana Cristina; Silbert, Suzane; Jones, Ronald N.; Sader, Hélio S.

doi:10.1086/501962

Cited by 22 publications

(18 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MLVA detected five main clusters of genetically related isolates from four Brazilian hospitals recovered during different periods. Persistence of enterococcal isolates sharing the same PFGE genotype and their intra-and interhospital spreading have also been described in previous investigations (1,10). In conclusion, MLVA accomplishes most of the desired typing features, including sufficient discriminatory power, speed, ease of use, and simple result interpretation.…”

supporting

confidence: 80%

Multilocus Variable-Number Tandem-Repeat Polymorphism among Brazilian Enterococcus faecalis Strains

et al. 2004

View full text Add to dashboard Cite

show abstract

supporting

confidence: 80%

Multilocus Variable-Number Tandem-Repeat Polymorphism among Brazilian Enterococcus faecalis Strains

et al. 2004

View full text Add to dashboard Cite

show abstract

“…First described in 1988 in France and England (1,2), glycopeptide-resistant enterococci have been isolated in Brazil since 1996, when the first case of vancomycin-resistant enterococci (VRE) due to a Enterococcus faecium VanD 4 was reported in Curitiba (Paraná State) (3,4). Further VRE isolates (E. faecium with the vanA gene) were reported in São Paulo State (5,6).…”

Section: Introductionmentioning

confidence: 99%

Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil

Camargo

Barth

Pilger

et al. 2004

Braz J Med Biol Res

View full text Add to dashboard Cite

In 2000, Enterococcus faecalis resistant to vancomycin was first reported at a tertiary hospital in Porto Alegre, southern Brazil. The resistance spread to other hospitals and surveillance programs were established by hospital infection committees to prevent the spread of vancomycin-resistant enterococci. In February 2002, an isolate initially identified at the genus level as Enterococcus was obtained by surveillance culture (rectal swab) from a patient admitted to a hospital for treatment of septic arthritis in the shoulder. The isolate proved to be resistant to vancomycin by the disc diffusion method and confirmed by an E-test resulting in a minimal inhibitory concentration of > or = 256 µg/ml. This isolate was sent to a reference laboratory (Laboratório Especial de Bacteriologia e Epidemiologia Molecular, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP) for further study and proved to be an E. gallinarum by the polymerase chain reaction (PCR) using specific primers for the species. Due to the phenotype of unusually high vancomycin resistance, the isolate presumably had the resistance genes (vanA and vanB) and this was confirmed by PCR, which indicated the presence of the vanA gene. A 10.8-kb Tn1546-related transposon was also identified by long-PCR. Interspecies transfer of the vancomycin-resistance gene from the donor E. gallinarum was performed in a successful conjugation experiment in vitro, using E. faecium GE-1 and E. faecalis JH22 as receptors. This is the first report of the detection of a vanA determinant naturally acquired by E. gallinarum in Brazil, indicating the importance of characterizing VRE by both phenotype and genotype methods

show abstract

“…In Brazil, the first case was reported in São Paulo [30]. Other cases occurred in different hospitals and, by 1998, VRE had spread throughout the country [31]. Disease severity controlled studies found that vancomycin resistance was predictive of increased mortality [32][33][34].…”

mentioning

confidence: 99%

Vancomycin use in a brazilian teaching hospital: comparison with the Hospital Infection Controlpractices Advisory Committee Guidelines (HICPAC)

Melo¹,

Ribeiro²

2009

Braz J Infect Dis

View full text Add to dashboard Cite

Molecular Typing and Antimicrobial Susceptibility of Vancomycin-ResistantEnterococcus faeciumin Brazil

Abstract: The results of our study indicate the occurrence of intra- and interhospital dissemination of VRE in Sao Paulo, Brazil.

Cited by 22 publications

References 13 publications

Multilocus Variable-Number Tandem-Repeat Polymorphism among Brazilian Enterococcus faecalis Strains

Multilocus Variable-Number Tandem-Repeat Polymorphism among Brazilian Enterococcus faecalis Strains

Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil

Vancomycin use in a brazilian teaching hospital: comparison with the Hospital Infection Controlpractices Advisory Committee Guidelines (HICPAC)

Contact Info

Product

Resources

About