Molecular typing and biological characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil

Stehling, Eliana Guedes; Leite, Domingos S.; Silveira, Wanderley Dias da

doi:10.1590/s1413-86702010000500007

Cited by 9 publications

(7 citation statements)

References 14 publications

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“…The isolates were initially analyzed by ERIC‐PCR to verify the genetic relationship among them, as well as to verify if clinical isolates were genetically more similar than environmental isolates. ERIC‐PCR is a rapid, reproducible, and highly discriminatory assay that proved to be a powerful surveillance screening tool for the typing of clinical P. aeruginosa isolated from patients with CF . Moreover, this technique appears to be a more reliable typing strategy for P. aeruginosa than other novel PCR‐based typing methodologies .…”

Section: Resultsmentioning

confidence: 99%

Pathogenic potential and genetic diversity of environmental and clinical isolates ofPseudomonas aeruginosa

Martins

Pitondo-Silva

Manço

et al. 2013

APMIS

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The aim of this study was to investigate the occurrence of virulence genes among clinical and environmental isolates of Pseudomonas aeruginosa and to establish their genetic relationships by Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR). A total of 60 P. aeruginosa isolates from environmental and clinical sources were studied. Of these, 20 bacterial isolates were from soil, 20 from water, and 20 from patients with cystic fibrosis. Analysis of ERIC-PCR demonstrated that the isolates of P. aeruginosa showed a considerable genetic variability, regardless of their habitat. Numerous virulence genes were detected in both clinical and environmental isolates, reinforcing the possible pathogenic potential of soil and water isolates. The results showed that the environmental P. aeruginosa has all the apparatus needed to cause disease in humans and animals.

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Section: Resultsmentioning

confidence: 99%

Pathogenic potential and genetic diversity of environmental and clinical isolates ofPseudomonas aeruginosa

Martins

Pitondo-Silva

Manço

et al. 2013

APMIS

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“…ERIC-PCR was used to determine the similarity between the isolates by the clonal relation ERIC2 primer (5'-AAGTAAGTGACTGGGGTGAGCG-3') [ 10 ]. The PCR cycle consisted of denaturation at 94°C for 5 min followed by 35 cycles at 94°C for 30 s, annealing at 52°C for 45 s, and extension at 72°C for 40 s. The obtained PCR fragments were electrophoresed in 2.0% agarose gel and the gel was analyzed using the GelJ v.1.3 software (GelJ company , San Diego, CA, USA) by considering a cutoff of 80.0% to discriminate the isolates.…”

Section: Methodsmentioning

confidence: 99%

The Spread of Insertion Sequences Element and Transposons in Carbapenem Resistant Acinetobacter baumannii in a Hospital Setting in Southwestern Iran

et al. 2022

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Background Acinetobacter baumannii is one of the most important hospital pathogenic bacteria that cause infectious diseases. The present study aimed to determine the frequency of carbapenem resistance genes in association with transposable elements and molecular typing of carbapenem-resistant A. baumannii bacteria collected from patients in Shiraz, Iran. Materials and Methods A total of 170 carbapenem-resistant A. baumannii isolates were obtained from different clinical specimens in two hospitals. The minimum inhibitory concentrations (MIC) of imipenem were determined and the prevalence of OXA Carbapenemases, Metallo-β-lactamases genes, insertion sequences (IS) elements, and transposons were evaluated by the polymerase chain reaction (PCR) method. Finally, molecular typing of the isolates was performed by the Enterobacterial Repetitive Intergenic Consensus-PCR method. Results The MICs ranged from 16 to 1,024 µg/mL for imipenem-resistant A. baumannii isolates. Out of the 170 carbapenem resistant A. baumannii isolates, bla OXA-24-like (94, 55.3%) followed by bla OXA-23-like (71, 41.7%) were predominant. In addition, A. baumannii isolates carried bla VIM (71, 41.7%), bla GES (32, 18.8%), bla SPM (4, 2.3%), and bla KPC (1, 0.6%). Moreover, IS Aba1 (94.2%) and Tn2009 (39.2%) were the most frequent transposable elements. Furthermore, (71, 44.0%) and (161, 94.7%) of the IS Aba1 of the isolates were associated with bla OXA-23 and bla OXA-51 genes, respectively. Besides (3, 1.7%), (1, 0.6%) and (5, 2.9%) of bla OXA-23 were associated with IS 18 , IS Aba4 , and IS Aba2 , respectively. Considering an 80.0% cut off, clusters and four singletons were detected. Conclusion According to the results, transposable elements played an important role in the development of resistance genes and resistance to carbapenems. The results also indicated carbapenem-resistant A. baumannii bacteria as a public health concern.

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“…Genomic DNAs of overnight cultured P. aeruginosa isolates were harvested using Bacterial DNA Isolation Kit (Foregene Biotechnology, Co. Ltd., China). Typing of P. aeruginosa isolates was performed by ERIC-PCR using the single primer 5’- AACTAAGTAACTGGGGTGAGCG-3’ (46). Phenotypic identification of P. aeruginosa isolates was performed as recommended by Filloux and Ramos (47) using PAO1 as positive control.…”

Section: Methodsmentioning

confidence: 99%

Modification of social cheating facilitates the stabilization and chronic infection of polymorphicPseudomonas aeruginosapopulation

Zhao

Yang

Zeng

et al. 2023

Preprint

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Chronic infection of the common bacterial pathogenPseudomonas aeruginosafrequently leads to the coexistence of heterogeneous individuals to engage in several group behaviors. However, further evolution of the polymorphicP. aeruginosapopulation, including the dynamic change of social cooperation and its impact on host immune system, still remain elusive. We show that the evolution ofP. aeruginosain the patients with chronic obstructive pulmonary disease frequently selects the isolates deficient in producing the costly and sharable extracellular products for nutrient acquisition. The evolution of polymorphicP. aeruginosapopulation is mainly concentrated on modifying the adaptability oflasRmutants, which are typical cheaters in the competition of quorum-sensing-controlled extracellular proteases. Importantly,lasRmutants with varying degrees of evolution interact with wild-typeP. aeruginosain a framework termed cascaded public goods game to compete for extracellular proteases and siderophores, and thus perpetuate social cooperation under different conditions. Finally, we find that a polymorphic population comprised oflasR-intactP. aeruginosaand evolvedlasR-mutant can minimize the host immune fluctuation for persistent colonization. This study demonstrates the multistage evolution and complex interaction ofP. aeruginosain adaptation to host lungs, and provides an explanation for the success of cooperation in public goods game.

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Molecular typing and biological characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil

Cited by 9 publications

References 14 publications

Pathogenic potential and genetic diversity of environmental and clinical isolates ofPseudomonas aeruginosa

Pathogenic potential and genetic diversity of environmental and clinical isolates ofPseudomonas aeruginosa

The Spread of Insertion Sequences Element and Transposons in Carbapenem Resistant Acinetobacter baumannii in a Hospital Setting in Southwestern Iran

Modification of social cheating facilitates the stabilization and chronic infection of polymorphicPseudomonas aeruginosapopulation

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