Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil

Cergole-Novella, Maria Cecília; Pignatari, Antônio Carlos Campos; Castanheira, Mariana; Guth, Beatriz E. C.

doi:10.1016/j.resmic.2010.09.022

Cited by 30 publications

(25 citation statements)

References 42 publications

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“…High rates of resistance to streptomycin and tetracycline have also been previously reported in STEC strains isolated from humans, cattle and food (Cergole‐Novella et al . ). The recent 2011 HUS outbreak in Germany was caused by a new strain of E. coli with a distinct set of virulence and antibiotic‐resistant factors (Bielaszewska et al .…”

Section: Discussionmentioning

confidence: 97%

Shiga toxin-producing Escherichia coli in drinking water supplies of north Paraná State, Brazil

et al. 2013

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Aim: To determine the occurrence and characteristics of Shiga toxinproducing Escherichia coli (STEC) in drinking water supplies treated and untreated. Methods and Results: Drinking water samples (n = 1850) were collected from 41 municipalities in the north of Paran a State between February 2005 and January 2006. Escherichia coli isolates (n = 300) were recovered from water and investigated for the presence of virulence markers related to STEC by PCR. STEC isolates recovered were then characterized for both phenotypic and genotypic traits. A total of 12 isolates (11 from untreated water and one from treated water) were positive for stx, including five positive for both stx1 and stx2, two positive for stx1 and five positive for stx2. None of the STEC isolates contained eae, but other virulence genes were observed such as ehxA (100%), saa (100%), lpfA O113 (75%), iha (42%), subAB (25%) and cdtV (8%). Multidrug resistance was identified in 25% of the STEC isolates. The 12 STEC isolates belonged to seven distinct serotypes and pulsed-field gel electrophoresis typing revealed the presence of two clusters and two clones in this region. Conclusion: Drinking water, especially from untreated water supplies, can be source of STEC strains potentially pathogenic for humans. Significance and Impact of the Study: The investigation of the drinking water supplies for pathogenic E. coli, as STEC, may be useful to prevent waterborne outbreaks.

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Section: Discussionmentioning

confidence: 97%

Shiga toxin-producing Escherichia coli in drinking water supplies of north Paraná State, Brazil

et al. 2013

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“…Plasmids in STEC often harbour antibiotic resistance gene cassettes on integrons (Colello et al, 2015;Singh et al, 2005;Van Meervenne et al, 2013;Zhao et al, 2001), and integron-positive strains have been found to be significantly more resistant to antibiotics compared to integron-negative strains (Van Meervenne et al, 2013). These integron-associated AR genes, such as aadA (aminoglycoside resistance), drfA1/dfrXII (trimethoprim resistance), sul1/sat1 (sulphonamides) and bla (against β-lactamase), can be transferred to other bacteria via plasmid conjugation (Cergole-Novella, Pignatari, Castanheira, & Guth, 2011;Kruger et al, 2015;Li, Wang, & Li, 2011;Maidhof et al, 2002;Nagachinta & Chen, 2008;Zhao et al, 2001). Class 1 integrons that contain a higher number of resistance gene cassettes than Class 2 integrons and confer better conjugation efficiency in bacteria harbouring them are found more often in STEC, as in other Gram-negative bacteria (Ahmed & Shimamoto, 2015;Colello et al, 2015;Gillings et al, 2008;Nagachinta & Chen, 2009;Vali et al, 2007).…”

Section: Acqu Is Iti On and Tr An S Miss I On Of Antib I Oti C Re Smentioning

confidence: 99%

Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

Mir

Kudva

2018

Zoonoses and Public Health

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Shiga toxin‐producing Escherichia coli (STEC) are foodborne pathogens that can cause severe diseases, including bloody diarrhoea and kidney failure, in humans, while remaining harmless to its primary reservoir hosts, cattle. Antibiotics such as azithromycin, fosfomycin and meropenem are being used and recommended in the treatment of early‐stage STEC (mainly E. coli O157:H7) infections, as these are reportedly effective in preventing Shiga toxin release and kidney failure while eliminating the pathogen. However, antibiotic resistance among STEC isolates could negatively impact these and other similar treatment options while contributing towards the spread of antibiotic resistance genes especially if encoded on mobile genetic elements like plasmids. Antibiotic resistance among STEC isolates recovered from animals and patients is being reported globally. A comprehensive understanding of the prevalence of antibiotic‐resistant STEC (AR‐STEC) and the mechanisms promoting this resistance among these bacteria could help direct therapies and develop strategies to effectively reduce/eliminate these pathogens. Here, we have reviewed literature from the past three decades to gain insights on this prevalence and its impact on human infections. In addition, we have reviewed various strategies proposed by researchers to control STEC that in turn would be applicable to AR‐STEC as well.

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“…One study, for example, estimated 18% of the 4,288 genes of Escherichia coli strain MG1655 were acquired laterally since the species diverged from the Salmonella lineage 100 million years (Myr) ago, a rate of 16 kb/Myr/lineage [59]. Transfer of mobile genetic elements between members of the Enterobacteriaceae is known to be responsible for the dissemination of antimicrobial resistance genes and the emergence of a variety of multidrug-resistant (MDR) Gram-negative bacteria globally [55,60,61]. …”

Section: Antimicrobial Resistance: a New Defense Strategy?mentioning

confidence: 99%

The Rising Dominance of Shigella sonnei: An Intercontinental Shift in the Etiology of Bacillary Dysentery

2015

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Shigellosis is the major global cause of dysentery. Shigella sonnei, which has historically been more commonly isolated in developed countries, is undergoing an unprecedented expansion across industrializing regions in Asia, Latin America, and the Middle East. The precise reasons underpinning the epidemiological distribution of the various Shigella species and this global surge in S. sonnei are unclear but may be due to three major environmental pressures. First, natural passive immunization with the bacterium Plesiomonas shigelloides is hypothesized to protect populations with poor water supplies against S. sonnei. Improving the quality of drinking water supplies would, therefore, result in a reduction in P. shigelloides exposure and a subsequent reduction in environmental immunization against S. sonnei. Secondly, the ubiquitous amoeba species Acanthamoeba castellanii has been shown to phagocytize S. sonnei efficiently and symbiotically, thus allowing the bacteria access to a protected niche in which to withstand chlorination and other harsh environmental conditions in temperate countries. Finally, S. sonnei has emerged from Europe and begun to spread globally only relatively recently. A strong selective pressure from localized antimicrobial use additionally appears to have had a dramatic impact on the evolution of the S. sonnei population. We hypothesize that S. sonnei, which exhibits an exceptional ability to acquire antimicrobial resistance genes from commensal and pathogenic bacteria, has a competitive advantage over S. flexneri, particularly in areas with poorly regulated antimicrobial use. Continuing improvement in the quality of global drinking water supplies alongside the rapid development of antimicrobial resistance predicts the burden and international distribution of S. sonnei will only continue to grow. An effective vaccine against S. sonnei is overdue and may become one of our only weapons against this increasingly dominant and problematic gastrointestinal pathogen.

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Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil

Cited by 30 publications

References 42 publications

Shiga toxin-producing Escherichia coli in drinking water supplies of north Paraná State, Brazil

Shiga toxin-producing Escherichia coli in drinking water supplies of north Paraná State, Brazil

Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

The Rising Dominance of Shigella sonnei: An Intercontinental Shift in the Etiology of Bacillary Dysentery

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