Molecular typing of clinical multidrug-resistant Klebsiella pneumoniae isolates

Kashefieh, Mehdi; Zeighami, Habib; Samadi Kafil, Hossein; Gholizadeh, Pourya; Sadeghi, Javid; Soroush Barhaghi, Mohammad Hossein; Ebrahimzadeh Leylabadlo, Hamed; Ghotaslou, Reza

doi:10.1007/s11033-024-09278-y

Cited by 1 publication

(2 citation statements)

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“…We also found that the CRISPR-Cas system-containing K. pneumoniae isolates belonged to different clusters, and the pattern of the distribution of the ESBL and aminoglycoside genes demonstrated that there was no significant association with ERIC clusters. However, in the Wasfi et al [ 60 ] and Kashefieh et al [ 63 ] studies, both ERIC-PCR and RAPD-PCR genotypic analyses demonstrated an association with resistance patterns of K. pneumonia . Even though RAPD-PCR and ERIC-PCR are quick, easy, and affordable genotyping techniques, their reproducibility is limited and contingent upon the PCR conditions and bacterial DNA quality [ 63 , 64 ].…”

Section: Discussionmentioning

confidence: 98%

“…However, in the Wasfi et al [ 60 ] and Kashefieh et al [ 63 ] studies, both ERIC-PCR and RAPD-PCR genotypic analyses demonstrated an association with resistance patterns of K. pneumonia . Even though RAPD-PCR and ERIC-PCR are quick, easy, and affordable genotyping techniques, their reproducibility is limited and contingent upon the PCR conditions and bacterial DNA quality [ 63 , 64 ]. Alternative typing techniques, like MLST, have been developed to achieve more dependable results.…”

Section: Discussionmentioning

confidence: 98%

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Prevalence of the CRISPR-cas system and its association with antibiotic resistance in clinical Klebsiella pneumoniae isolates

Kadkhoda,

Gholizadeh,

Ghotaslou

et al. 2024

BMC Infect Dis

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Background and objective(s) CRISPR-Cas is a prokaryotic adaptive immune system that protects bacteria and archaea against mobile genetic elements (MGEs) such as bacteriophages plasmids, and transposons. In this study, we aimed to assess the prevalence of the CRISPR-Cas systems and their association with antibiotic resistance in one of the most challenging bacterial pathogens, Klebsiella pneumoniae. Materials and methods A total of 105 K. pneumoniae isolates were collected from various clinical infections. Extended-spectrum β-lactamases (ESBLs) phenotypically were detected and the presence of ESBL, aminoglycoside-modifying enzymes (AME), and CRISPR-Cas system subtype genes were identified using PCR. Moreover, the diversity of the isolates was determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results Phenotypically, 41.9% (44/105) of the isolates were found to be ESBL producers. A significant inverse correlation existed between the subtype I-E CRISPR-Cas system’s presence and ESBL production in K. pneumoniae isolates. Additionally, the frequency of the ESBL genes blaCTX−M1 (3%), blaCTX−M9 (12.1%), blaSHV (51.5%), and blaTEM (33.3%), as well as some AME genes such as aac(3)-Iva (21.2%) and ant(2’’)-Ia (3%) was significantly lower in the isolates with the subtype I-E CRISPR-Cas system in comparison to CRISPR-negative isolates. There was a significant inverse correlation between the presence of ESBL and some AME genes with subtype I-E CRISPR-Cas system. Conclusion The presence of the subtype I-E CRISPR-Cas system was correlated with the antibiotic-resistant gene (ARGs). The isolates with subtype I-E CRISPR-Cas system had a lower frequency of ESBL genes and some AME genes than CRISPR-negative isolates.

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Section: Discussionmentioning

confidence: 98%