Molecular Typing of Clostridium perfringens Isolates from Faecal Samples of Healthy and Diarrhoeic Sheep and Goats in Kashmir

Rasool, Sumiyah; Hussain, Imtiaz; Wani, S.A.; Kashoo, Zahid Amin; Beigh, Qusina; Nyrah, Qazi; Nazir, Nageena; Hussain, Tufail; Wani, Abdul Hamid; Qureshi, Sabia

doi:10.20546/ijcmas.2017.610.372

Cited by 4 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study the antibiotic susceptibility profile of C. perfringens type A and F were showed resistance for different families of antibiotics. Phenotypically, our findings were observed a high multidrug resistance (resistance ≥ three classes of antibiotic), these results in consistency to previous study (Wen et al, 2018;Khan et al,2019;Hussain et al,.2017;Rahaman et al,2013;Jang et al ,2020;Elgoas et al,2020;Ahmed et al, 2022).…”

Section: Resultssupporting

confidence: 93%

“…Results of multiplex PCR for detection of toxigenic genes revealed that cpa gene which responsible for type A ( positive only for alpha-toxin) was the predominant gene (90%) in apparently healthy and dead cases of sheep and goat, type A associated with food poisoning worldwide (Guran and Oksuztepe, 2013). Many studies reported the superiority of type A with percentage reached to100 % (Mignaqui et al,2017 ;Hayati and Tahamtan,2021;Rasool et al, 2017 ;Karunakarnan et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of Clostridium perfringens in small ruminants

Mohamed,

Elfeky,

Abd Al-Azeem

et al. 2023

SVU-International Journal of Veterinary Sciences

View full text Add to dashboard Cite

A significant foodborne pathogen as Clostridium perfringens (C. perfringens) has been linked to many diseases in sheep and goat, included; Enterotoxaemia, pulpy kidney disease,struck, and even animal mortality. So, The objective of this study is to discuss the presence of C. perfringens in small ruminants with focusing on the virulence factors, antimicrobial resistance profile, and biofilmforming capacities of the organism. A total 166 samples, (95 from sheep, 71 from goats) collected from apparently healthy, diarrheic animals and dead carcasses suspected to be infected with enterotoxaemia in Sohag, Egypt. These samples were subjected for Animal health research institute lab for microbiological examination. The phenotypic identification revealed that 25 isolates (15.1%) were Clostridium spp. 10 isolates were identified as C. perfringens by PCR using a species-specific 16S rRNA gene. The results of multiplex PCR, revealed that the cpa gene, which is responsible for C. perfringens toxintype A, had the highest prevalence (90%) followed by the cpe gene, which is responsible for type F, while none of the isolates had the cpb, etx, and iap genes. Phenotypically, the majority of the isolates displayed multidrug resistance (MDR) patterns for, vancomycin, tetracycline , oxacillin, and piperacillin,. erythromycin and ceftazidime and whereas genetically, our isolates had the bla(70%), ermB (40%) and tetK (30%) genes. Our isolates demonstrated a moderate (10%) and weak (60%) ability to form biofilm.

show abstract

Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Molecular characterization of Clostridium perfringens in small ruminants

Mohamed,

Elfeky,

Abd Al-Azeem

et al. 2023

SVU-International Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…1998). Recent reports by Rasool et al (2017) reported prevalence of C. perfringens to the tune of 44.94% from sheep in Kashmir valley where type A was most prevalent corroborating with our study. Similar study in this region by Nazki et al (2017) reported prevalence of 72.36% from sheep.…”

Section: Resultssupporting

confidence: 92%

“…2014). Recent studies by Rasool et al (2017) and Nazki et al (2017) also reported that majority of type D isolates were from diarrhoeic lambs occurrence of 58.6% and 56.16% respectively of type D isolates which endorse our results. Clostridium perfringens isolates obtained in this study were screened for presence of three toxin genes viz., cpa, cpb and etx.…”

Section: Resultssupporting

confidence: 91%

Molecular Characterisation of Clostridium perfringens Type D Isolated from Sheep in Kashmir Himalayas, India

Kashoo¹,

Wani²,

Wani³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

Self Cite

View full text Add to dashboard Cite

“…The results of cpb2 in their study was in agreement with the present findings with similar agreement. Park et al (2015) and Balchandran et al (2018) recorded 100 percent prevalence of cpa toxin in the isolates of C. perfringens from NE cases while Rasool et al (2017) reported 86.4 percent prevalence of cpa gene, but the prevalence of cpa gene in the present study was recorded less as compared to other studies. The possibility of the same may be due to the presence of other toxin gene which were prominently expressed and reduced the expression of the cpa gene.…”

Section: Toxinotyping Of C Perfringenscontrasting

confidence: 66%

Toxinotyping of Clostridium perfringens in Poultry from Necrotic Enteritis Cases

Shelke¹

2020

JAR

View full text Add to dashboard Cite

Clostridium perfringens is well known causative agent of necrotic enteritis in poultry and is mainly caused by Type A toxin. NetB toxin is found to be one of the newly emerging virulent toxin gene which is also responsible for necrotic enteritis. The present study was carried out to characterize and to type the different toxins associated with C. perfringens in NE cases of poultry. For the present study total 125 samples were collected from poultry birds, out of which 50 samples were of intestinal content from diseased birds, 50 cloacal swabs and 25 intestinal content from healthy birds. These samples were further processed for isolation, identification, and toxinotyping of Clostridium perfringens isolates. Onisolation of C. perfringens on blood agar total 43 isolates were found positive showing a pattern of double hemolysis on blood agar. The positive isolates of C. perfringens were further confirmed by using 16S rRNA species specific PCR. After confirmation isolates were processed for toxinotyping mainly targeting cpa, cpb and cpb2 toxins by using multiplex PCR. On toxinotyping it was found that NE in poultry birds were mainly caused by C. perfringens type A. On virulent gene detection of netB toxin, total 4 isolates were found positive for netB toxin. This study pointed out that C. perfringens type A is responsible for development of NE in poultry along with net B toxin which is a new key virulent factor. Further studies of netB toxoid and C. perfringens type A for vaccine production could minimize the clostridial problems in broiler farms.

show abstract

Molecular Typing of Clostridium perfringens Isolates from Faecal Samples of Healthy and Diarrhoeic Sheep and Goats in Kashmir

Cited by 4 publications

References 14 publications

Molecular characterization of Clostridium perfringens in small ruminants

Molecular characterization of Clostridium perfringens in small ruminants

Molecular Characterisation of Clostridium perfringens Type D Isolated from Sheep in Kashmir Himalayas, India

Toxinotyping of Clostridium perfringens in Poultry from Necrotic Enteritis Cases

Contact Info

Product

Resources

About