Molecular Typing of Eimeria ahsata and E. crandallis Isolated From Slaughterhouse Wastewater

Hatam-Nahavandi, Kareem; Mahvi, Amir Hossein; Mohebali, Mehdi; Keshavarz, Hossein; Rezaei, Satar; Mirjalali, Hamed; Elikaei, Samira; Rezaeian, Mostafa

doi:10.5812/jjm.34140

Cited by 17 publications

(18 citation statements)

References 10 publications

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“…Cryptosporidium was identified in 4 out of 707 school children from Turkey (0.6%); all the isolates belonged to the C. parvum bovine genotype (34). The current results are consistent with the reports from Saudi Arabia, Iran, Kuwait, and Turkey (8,(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36).…”

Section: Discussionsupporting

confidence: 92%

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Cryptosporidium parvum in Children with Diarrhea in Zahedan, Iran

Mirshekari

Hatam-Nahavandi

Khabisi

et al. 2019

Jundishapur J Microbiol

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Background: Cryptosporidium is one of the most important causes of gastroenteritis in humans. Objectives: This study aimed to investigate the distribution of Cryptosporidium species in children with acute diarrheal disease in Zahedan, Iran. Methods: Stool specimens collected from 764 children aged < 10 years with diarrheal disease admitted to Aliasghar Pediatric Hospital and the Reference Laboratory in Zahedan were assessed for the presence of Cryptosporidium oocysts using the conventional microscopic examination and acid-fast staining. To characterize the oocysts at the molecular level, the 18S rRNA was nested-PCR amplified and sequenced. PCR products were also digested using Vsp1 restriction enzyme for genotype differentiation. Results: Of the 764 stool specimens, 7 (0.91%) were positive for Cryptosporidium oocysts using the microscopic examination. Restriction endonuclease digestion pattern successfully revealed the presence of Cryptosporidium parvum in two microscopic positive samples that were confirmed by DNA sequencing. Conclusions: Cryptosporidium parvum is responsible for cryptosporidiosis in children under 10 years of age in the study region. Although C. parvum may be an important pathogen associated with diarrhea, it is the cause of only a small proportion of diarrheal episodes.

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Section: Discussionsupporting

confidence: 92%

“…The rate of positivity was lower in PCR than in microscopic examination. Previous studies also reported similar findings, which might be attributed to DNA inadequacy due to the low number of oocysts in stool samples (26)(27)(28).…”

Section: Discussionsupporting

confidence: 69%

Cryptosporidium parvum in Children with Diarrhea in Zahedan, Iran

Mirshekari

Hatam-Nahavandi

Khabisi

et al. 2019

Jundishapur J Microbiol

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“…The sediment related to each sample was washed by phosphate-buffered saline (PBS; pH: 6.4) three times at 8000 rpm for 60 seconds. The DNA extraction was performed using an Exgene stool SV kit (GeneAll Biotechnology Co. Ltd, South Korea) as instructed by the manufacturer (13)(14)(15).…”

Section: Methodsmentioning

confidence: 99%

Trichomoniasis Prevalence at a Care Center Among Women with High-Risk Behaviors in Zahedan, Iran

Haghighi

Jafari-Modrek

Sohrabi

et al. 2019

Int J High Risk Behav Addict

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Background: Trichomoniasis is a sexually transmitted infection (STI) attributed to Trichomonas vaginalis, an anaerobic, flagellated protozoan parasite. Although trichomonacidal drugs effectively kill Trichomonas, the infection rate is still high. Objectives: The aim of the present study was to investigate the frequency of T. vaginalis among women with high-risk behaviors in Zahedan, Southeast Iran. Patients and Methods: In total, 90 symptomatic women, aged 20 to 60 years, were recruited between February and May 2017. The samples were smeared and cultured. After extracting DNA, the actin gene was amplified via nested polymerase chain reaction (PCR). Results: Of the 90 samples including vaginal secretions and urine specimens, 23.3% (21/90) were positive for T. vaginalis based on wet mount microscopy. All wet mount positive samples were also T. vaginalis positive by PCR and cultivation. The highest prevalence of trichomoniasis was observed at the age of 31 to 40 years (38.09%). Trichomoniasis showed no significant difference by age groups (P > 0.05 on the chi-square test). Four PCR-positive samples were successfully analyzed by RFLP, which were associated with genotype E. Conclusions: The current research reflected a high level of infection in women with high-risk behaviors in Zahedan, southeastern Iran. To clarify the epidemiology of disease in this area, more studies in other populations are required.

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“…Furthermore, the morphological similarity of oocysts of some Eimeria spp. is a limitation in supporting (or refuting) identification based on microscopy alone (Tenter et al, 2002;Haug et al, 2007;Kawahara et al, 2010;Hatam-Nahavandi et al, 2016). For example, Eimeria spp.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…The combined use of morphological and molecular tools offers advantages in confirming Eimeria spp. identification (Kawahara et al, 2010;Hatam-Nahavandi et al, 2016), particularly with respect to speciation for morphologically similar Eimeria spp. oocysts in faecal samples.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Morphological and molecular characterization of three Eimeria species from captured rangeland goats in Western Australia

Al-Habsi

Yang

Ryan

et al. 2017

Veterinary Parasitology: Regional Studies and Reports

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2017)Morphological and molecular characterization of three Eimeria species from captured rangeland goats in Western Australia. Veterinary Parasitology: Regional Studies and Reports, 9. pp. 75-83. ABSTRACT Faecal shedding of Eimeria by captured rangeland goats (Capra hircus) was investigated using a longitudinal observational study. Faecal samples were collected from 125 male goats on four occasions. The first sampling occurred following capture and transport, immediately after arrival at a commercial goat depot (feedlot) in Western Australia, with subsequent 3 sample collections occurring at one month intervals thereafter. Goats were composite breed and aged approximately 9-12 months on arrival at the feedlot. Prevalence and shedding intensity (faecal oocyst concentration) for Eimeria were determined using qPCR. Species were identified from individual oocysts (isolated using micromanipulation) using molecular analysis at two loci, specifically 18S rRNA and mitochondrial cytochrome oxidase gene (COI), and confirmed by microscopy. Longitudinal prevalence (animals positive at least once) for Eimeria spp. by qPCR was 90.4%, with 60% goats shedding Eimeria spp. on more than one occasion. Point prevalence (prevalence at a single sampling occasion) ranged from 2.4% (fourth sampling) to 70.4% (second sampling). Three species were identified at the 18S rRNA locus and confirmed by microscopy: E. christenseni (longitudinal prevalence for single infection 34.4%), E. hirci (17.6%) and E. arloingi (8.8%) over the four sample collections. Mixed infections were identified in 56.8% goats (longitudinal prevalence). 18S rRNA sequences from E. christenseni and E. hirci and were 100% homologous with ovine E. ahsata and E. crandallis respectively, and E. arloingi was 100% similar to caprine E. arloingi. At the COI locus, E. christenseni, E. hirci and E. arloingi grouped separately, and were closely related to ovine E. ahsata, with genetic similarities of 96.5%, 92.6% and 91.4% respectively. This is the first report for molecular characteristics of caprine-derived Eimeria spp. using a combination of 18S rRNA and COI. Molecular techniques can be used to identify Eimeria ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T spp. in goat faecal samples, specifically through characterization at 18S locus and other gene loci when used in parallel. Molecular techniques offer some advantages over microscopy for identification of Eimeria species, particularly with respect to precision.

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Molecular Typing of Eimeria ahsata and E. crandallis Isolated From Slaughterhouse Wastewater

Cited by 17 publications

References 10 publications

Cryptosporidium parvum in Children with Diarrhea in Zahedan, Iran

Cryptosporidium parvum in Children with Diarrhea in Zahedan, Iran

Trichomoniasis Prevalence at a Care Center Among Women with High-Risk Behaviors in Zahedan, Iran

Morphological and molecular characterization of three Eimeria species from captured rangeland goats in Western Australia

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