Molecular Typing of Fluoroquinolone-ResistantCampylobacter jejuniIsolated from Broilers in Japan Using Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis

Abstract

Fluoroquinolone-resistant Campylobacter jejuni isolates from broilers in Japan were characterized using multilocus sequence typing and pulsed-field gel electrophoresis (PFGE) in order to elucidate the genetic relationship between these strains. Forty-three of the isolates were classified into 20 sequence types and were clustered into 21 PFGE types with 70% similarity. The most dominant clonal complex (CC) was CC-21 (41.9%). Diverse PFGE patterns were observed within the same CC, but the combined analysis of PFGE type and CC revealed that the strains with the same combination were isolated from the same district or neighboring districts. On the other hand, strains with the same combination pattern were also isolated from geographically distant districts. Our results elucidate two possible reasons for the prevalence of fluoroquinolone-resistant C. jejuni among broiler farms: (1) the resistant C. jejuni is clonally disseminated within the limited area, and (2) susceptible C. jejuni acquired fluoroquinolone resistance during the use of fluoroquinolone on the farms.

Campylobacter multi‐locus sequence types and antimicrobial susceptibility of broiler cecal isolates: A two year study of 143 commercial flocks

“…Furthermore, CCs 353, 828, and 48 were the three most common clonal complexes detected associated with packaged chicken meat at purchased at retail in a market survey conducted in Georgia, United States (Berrang, Oakley, & Meinersmann, ). In contrast, the most commonly found ST CC (in order of prevalence) in Great Britain (Jorgensen et al, ) and Finland (Llarena et al, ) are CC‐45 and CC‐21; in Sweden, CC‐21 and CC‐45 (Griekspoor, Engvall, Olsen, & Waldenstrom, ); and in Japan CC‐21 and CC‐48 (Ozawa et al, ). While CC‐353 is commonly found in other geographic locations, its' prevalence accounted for only 2.2, 1.0, and 4.7% of the isolates recovered from poultry in Great Britain, Sweden, and Japan, respectively (Griekspoor et al, ; Jorgensen et al, ; Ozawa et al, ).…”

Campylobacter and Salmonella are prevalent in broiler farms in Kyushu, Japan: results of a 2-year distribution and circulation dynamics audit

“…; Ozawa et al . ). MLST data for Japanese broiler farms are lacking; however, hampering the comparisons of ecology and distribution at the farm level with data from other countries.…”

“…Multi locus sequence typing (MLST) holds promise for the molecular epidemiological analysis of Campylobacter and Salmonella species, and has been frequently applied in epidemiological analysis (Actman et al, 2013;Asakura et al 2013;Dingle et al 2001;Liu et al 2011;Llarena et al 2015;Ozawa et al 2016). MLST data for Japanese broiler farms are lacking; however, hampering the comparisons of ecology and distribution at the farm level with data from other countries.…”

Cronobacter sakazakii and Microbiological Parameters in Dairy Formulas Associated With a Food Alert in Chile

“…Several primers have been generated to detect Cronobacter spp. by amplifying specific sequences of variable and conserved regions of 16S ribosomal rDNA of the bacterium ( Lehner et al, 2004 ; Hassan et al, 2007 ), OmpA ( Mohan-Nair and Venkitanarayanan, 2006 ), as well as others that are more specific, which detect C. sakazakii by rpoB ( Stoop et al, 2009 ; Lehner et al, 2012 ; Li et al, 2016 ) and cgcA gene amplification ( Carter et al, 2013 ).…”