Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity

Kaján, Gyözö L.; Kecskeméti, Sándor; Harrach, Balázs; Benkő, Mária

doi:10.1016/j.vetmic.2013.09.025

Cited by 104 publications

(86 citation statements)

References 30 publications

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“…These results are similar to results from Hungary [7], where isolated adenovirus strains frequently belonged to the species Fowl aviadenovirus D or Fowl aviadenovirus E. Serotype FAdV-1 was even more infrequent [8]. The rarest serotype isolated in Poland was serotype FAdV-4, which was found in only 2.9 % of the examined strains; however, it was never isolated in Hungary [7]. On the other hand, serotypes FAdV-3, FAdV-6, FAdV-9, and FAdV-10 have not been identified in Poland.…”

Section: Discussionsupporting

confidence: 91%

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Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Niczyporuk

2015

Arch Virol

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Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbreaks and developing strategies for vaccine development. Several molecular epidemiology and phylogenetic studies have been performed, but the results obtained are still limited, because FAdV strains, even of the same serotype, have very diverse characteristics. Some strains are pathogenic and some are nonpathogenic. This report describes the successful isolation of 96 FAdV field strains from chickens in Poland. A PCR assay specific for the L1 loop region of the hexon gene was conducted, and the products were subjected to sequence analysis. The sequences were analysed using BLAST and Geneious 6.0 software and compared to adenovirus field and reference strain sequences from different parts of the world that are accessible in the NCBI GenBank database. The sequences of the adenovirus strains indicated that they belonged to five species, Fowl aviadenovirus A-E, represented by eight serotypes FAdV-1, FAdV-4, FAdV-5, FAdV-7, FAdV-8a, FAdV-8b, and FAdV-2/11 (FAdV-D). The relationships between FAdVs isolated in Poland and isolates from other regions of the world were determined.

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Section: Discussionsupporting

confidence: 91%

“…The similarity of nucleotide sequences of strains belonging to the same serotype (within the group) was between 89.7 % and 93.0 %, which was comparable to the data reported by Kajan et al [7]. However, differences among strains representing different species were found.…”

Section: Discussionsupporting

confidence: 88%

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Niczyporuk

2015

Arch Virol

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“…During the last 15 years some FAdV-1 strains have been shown to cause gizzard erosions in broiler chickens in Japan (Abe et al, 2001;Okuda et al, 2001;Nakamura et al, 2002;Ono et al, 2003), several European countries (Manarolla et al, 2009;Marek et al, 2010;Domanska-Blicharz et al, 2011;Grafl et al, 2012;Kaján et al, 2013;Schade et al, 2013) and in Korea (Lim et al, 2012). The FAdV-1 field isolate used in this study was isolated from the bursa of a healthy broiler chicken, as in other studies in which serotype 1 isolates were obtained from healthy birds Nakamura et al, 2002).…”

Section: Discussionmentioning

confidence: 95%

Chronological analysis of gross and histological lesions induced by field strains of fowl adenovirus serotypes 1, 8b and 11 in one-day-old chickens

et al. 2015

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Fowl adenoviruses (FAdVs) cause diseases in domestic chickens, including inclusion body hepatitis (IBH), with immunosuppression believed to play a role in their pathogenesis. To gain a better understanding of the pathogenesis and chronology of disease caused by FAdVs, the gross pathology, histopathology and dissemination of virus were examined at several different time points, after inoculation of one-day-old specific pathogen-free chickens with FAdV-1, FAdV-8b or FAdV-11 via the ocular route. FAdV-8b had a slightly greater virulence than FAdV-11, but both were primary pathogens. The presence and severity of hepatic lesions were used to define the three stages of the disease: incubation (1-3 days post-inoculation, PI), degeneration (4-7 days PI) and convalescence (14 days PI). Both viruses were detected in the liver, kidney, bursa, thymus and gizzard of most birds during the degenerative stage, and persisted in the gizzard into convalescence. The FAdV-1 isolate was found to be apathogenic, but virus was detected in the bursa and/or gizzard of several birds between 2 and 7 days PI. This is the first study examining the chronology of gross and microscopic lesions of pathogenic and apathogenic FAdVs in association with viral presence in multiple tissues. It was concluded that both FAdV-8b and FAdV-11 are primary pathogens, and that these strains may play a role in immunosuppression.

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“…The full genome sequence of the FAdV-1-61/11z virus strain was reported by Matczuk et al in 2017 [22]. Interestingly, some strains of FAdV-8a may be implicated in the development of GEU [16,17,19] and IBH [14,21,23,24,37]. Adenoviruses have been suggested to play a role in immunodeficiency [24,28,35].…”

Section: Discussionmentioning

confidence: 99%

A comparative pathogenicity analysis of two adenovirus strains, 1/A and 8a/E, isolated from poultry in Poland

Niczyporuk

Czekaj

2018

Arch Virol

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Fowl adenoviruses (FAdVs) are the causative agents of multietiological syndromes and diseases in poultry flocks. During a routine diagnostic examination, two FAdVs strains were isolated. Molecular typing of these isolates based on the partial loop L1 HVR1-4 region of the hexon gene sequence revealed the presence of different FAdV isolates: 1/A-61/11z (GenBank accession number KX247012, APP94082), and 8a/E-6/12j (GenBank accession number: KP890032, ALB00550), and comparative genome analysis indicated small differences between these two viruses. The next step of the study was the estimation of the pathogenicity of these isolates in specific-pathogen-free (SPF) chickens. Chickens were divided into three groups, with 20 chickens per group infected intraperitoneally on the first day after hatching. Group I consisted of chickens infected with strain FAdV-1/A-61/11z, group II consisted of chickens infected with strain FAdV-8a/E-6/12j, and group III consisted of uninfected birds. Clinical signs observed in infected chickens included poor growth, apathy, prostration, ruffled feathers, crouching position, and huddling behavior. The mortality rate in chickens infected with FAdV-1/A-61/11z was 10% at 10 days postinfection (dpi), and no mortality was observed in chickens infected with the FAdV-8a-6/12j strain. The mean real-time PCR threshold cycle (Ct) value was 39.70%. The detection limit of these assays was 8 copies, with an efficiency of 91.03% and 95.17% and regression square (R2) values of 0.991 and 0.997, respectively, with a mean pathogen load of 4.8 × 10 6.0 copies/µl. The assays did not demonstrate cross-reactivity between types 1/A and 8a/E and non-targeted poultry viruses. Adenoviral DNA was detected in the liver, spleen, kidney, gizzard, intestine, bursa of Fabricius, and thymus of every examined dead and euthanized chicken from groups I and II between the third and fourth week postinfection. This is the first study conducted on the pathogenic and apathogenic strains FAdV-1/A and FAdV-8a/E, showing the presence of the virus in multiple tissues in chickens in Poland. This study revealed that it is very likely that the FAdV-1/A-61/11z strain is able to cause clinical inclusion body hepatitis (IBH) in chickens and that it is slightly more virulent than the FAdV-8a/E-6/12j strain, although both are primary pathogens of the disease.

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Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity

Cited by 104 publications

References 30 publications

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Chronological analysis of gross and histological lesions induced by field strains of fowl adenovirus serotypes 1, 8b and 11 in one-day-old chickens

A comparative pathogenicity analysis of two adenovirus strains, 1/A and 8a/E, isolated from poultry in Poland

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