Molecular typing of <i>Escherichia coli</i> strains associated with threatened sea ducks and near‐shore marine habitats of south‐west Alaska

Hollmén, Tuula E.; DebRoy, Chitrita; Flint, Paul L.; Safine, David E.; Schamber, Jason L.; Riddle, Ann E.; Trust, Kimberly A.

doi:10.1111/j.1758-2229.2010.00220.x

Cited by 3 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular investigations have been carried using a number of typing techniques including enterobacterial repetitive intergenic consensus (ERIC), random amplified polymorphic deoxyribonucleic acid (RAPD), repetitive extragenic palindromic (REP), pulse-field gel electrophoresis, and multi-locus sequence typing [23][24][25][26][27]. Recent advances have promoted the use of whole-genome sequencing and metagenomics.…”

Section: Introductionmentioning

confidence: 99%

Genomic Investigation into the Virulome, Pathogenicity, Stress Response Factors, Clonal Lineages, and Phylogenetic Relationship of Escherichia coli Strains Isolated from Meat Sources in Ghana

Adzitey

Asante

Kumalo

et al. 2020

Genes

View full text Add to dashboard Cite

Escherichia coli are among the most common foodborne pathogens associated with infections reported from meat sources. This study investigated the virulome, pathogenicity, stress response factors, clonal lineages, and the phylogenomic relationship of E. coli isolated from different meat sources in Ghana using whole-genome sequencing. Isolates were screened from five meat sources (beef, chevon, guinea fowl, local chicken, and mutton) and five areas (Aboabo, Central market, Nyorni, Victory cinema, and Tishegu) based in the Tamale Metropolis, Ghana. Following microbial identification, the E. coli strains were subjected to whole-genome sequencing. Comparative visualisation analyses showed different DNA synteny of the strains. The isolates consisted of diverse sequence types (STs) with the most common being ST155 (n = 3/14). Based Upon Related Sequence Types (eBURST) analyses of the study sequence types identified four similar clones, five single-locus variants, and two satellite clones (more distantly) with global curated E. coli STs. All the isolates possessed at least one restriction-modification (R-M) and CRISPR defence system. Further analysis revealed conserved stress response mechanisms (detoxification, osmotic, oxidative, and periplasmic stress) in the strains. Estimation of pathogenicity predicted a higher average probability score (Pscore ≈ 0.937), supporting their pathogenic potential to humans. Diverse virulence genes that were clonal-specific were identified. Phylogenomic tree analyses coupled with metadata insights depicted the high genetic diversity of the E. coli isolates with no correlation with their meat sources and areas. The findings of this bioinformatic analyses further our understanding of E. coli in meat sources and are broadly relevant to the design of contamination control strategies in meat retail settings in Ghana.

show abstract

Section: Introductionmentioning

confidence: 99%

Genomic Investigation into the Virulome, Pathogenicity, Stress Response Factors, Clonal Lineages, and Phylogenetic Relationship of Escherichia coli Strains Isolated from Meat Sources in Ghana

Adzitey

Asante

Kumalo

et al. 2020

Genes

View full text Add to dashboard Cite

show abstract

Genetic Diversity of Escherichia coli Isolated from Ducks and the Environment Using Enterobacterial Repetitive Intergenic Consensus

Adzitey

2013

Pakistan J. of Biological Sciences

View full text Add to dashboard Cite

Escherichia coli are mostly free living bacteria that harbour the gastrointestinal tract of poultry. Howbeit, pathogenic Escherichia coli are very important foodborne pathogens that can cause severe complications, illnesses and deaths in humans. The objective of this study was to determine the genetic diversity or relatedness of 62 Escherichia coli strains isolated from ducks and the environment using Enterobacterial Repetitive Intergenic Consensus (ERIC). The analysis of the Escherichia coli strains by ERIC produced DNA bands of different sizes for differentiation purposes and cluster analysis at a coefficient of 0.85 grouped the strains into different clusters and singletons. At this coefficient the Escherichia coli strains were grouped into thirteen clusters and eleven singletons with discriminatory index (D value) of 0.946. The ERIC PCR adapted in this study showed to be a useful genotyping tool for determining the genetic relatedness of the duck Escherichia coli strains. Comparison of the genetic relatedness among foodborne pathogens is important for foodborne diseases outbreak investigations.

show abstract

Trace Elements in Blood of Sea Ducks from Dutch Harbor and Izembek Lagoon, Alaska

Franson

Hollmén

Flint

et al. 2022

Journal of Fish and Wildlife Management

View full text Add to dashboard Cite

In 2001, we collected whole blood from sea ducks (Steller’s eider Polysticta stelleri, harlequin duck Histrionicus histrionicus, black scoter Melanitta nigra, and long-tailed duck Clangula hyemalis) wintering at Dutch Harbor, Alaska, and from Steller’s eiders molting at Izembek Lagoon on the Alaska Peninsula. Blood samples were analyzed for 19 trace elements, of which 17 were detected in one or more samples. In Steller’s eiders, mean concentrations of six trace elements (As, B, Fe, Hg, Se, Mo) were greater at Dutch Harbor and mean concentrations of four trace elements (Cr, Cu, Mg, Zn) were greater at Izembek Lagoon. Among sea ducks at Dutch Harbor, mean concentrations of five trace elements (Cu, Hg, Se, Zn, V) differed by species. Steller’s eiders had greater concentrations of Cu, Zn, and V in their blood, black scoters had the highest Se, and harlequin ducks had the highest Hg, with a mean concentration slightly above a threshold effect level. One Steller’s eider and one harlequin duck from Dutch Harbor had blood Pb levels above background concentrations. We have no observations to indicate that concentrations of these trace elements were associated with adverse effects.

show abstract

Molecular typing of Escherichia coli strains associated with threatened sea ducks and near‐shore marine habitats of south‐west Alaska

Cited by 3 publications

References 27 publications

Genomic Investigation into the Virulome, Pathogenicity, Stress Response Factors, Clonal Lineages, and Phylogenetic Relationship of Escherichia coli Strains Isolated from Meat Sources in Ghana

Genomic Investigation into the Virulome, Pathogenicity, Stress Response Factors, Clonal Lineages, and Phylogenetic Relationship of Escherichia coli Strains Isolated from Meat Sources in Ghana

Genetic Diversity of Escherichia coli Isolated from Ducks and the Environment Using Enterobacterial Repetitive Intergenic Consensus

Trace Elements in Blood of Sea Ducks from Dutch Harbor and Izembek Lagoon, Alaska

Contact Info

Product

Resources

About