Molecular typing of <i>Mycobacterium bovis</i> isolated from tuberculosis lesions of cattle in north eastern Ethiopia

Ameni, Gobena; Desta, Fekadu Shiferaw; Firdessa, Rebuma

doi:10.1136/vr.b4881

Cited by 46 publications

(38 citation statements)

References 29 publications

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“…The distribution, frequency, and severity of lesions recorded in different tissues of camels were similar with the reports of similar studies in grazing cattle in Ethiopia [18], [32]. In these studies, the frequency and severity of the lesions were higher in the mesenteric lymph nodes than the thoracic lymph nodes, while in other studies under intensive cattle husbandry lesions were predominant in the respiratory tract and thoracic lymph nodes [14], [16].…”

Section: Discussionsupporting

confidence: 86%

Pathology of Camel Tuberculosis and Molecular Characterization of Its Causative Agents in Pastoral Regions of Ethiopia

et al. 2011

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A cross sectional study was conducted on 906 apparently healthy camels slaughtered at Akaki and Metehara abattoirs to investigate the pathology of camel tuberculosis (TB) and characterize its causative agents using postmortem examination, mycobacteriological culturing, and multiplex polymerase chain reaction (PCR), region of difference-4 (RD4)-based PCR and spoligotyping. The prevalence of camel TB was 10.04% (91/906) on the basis of pathology and it was significantly higher in females (χ2 = 4.789; P = 0.029). The tropism of TB lesions was significantly different among the lymph nodes (χ2 = 22.697; P = 0.002) and lung lobes (χ2 = 17.901; P = 0.006). Mycobacterial growth was observed in 34% (31/91) of camels with grossly suspicious TB lesions. Upon further molecular characterization using multiplex PCR, 68% (21/31) of the colonies showed a positive signal for the genus Mycobacterium, of which two were confirmed Mycobacterium bovis (M. bovis) by RD4 deletion typing. Further characterization of the two M. bovis at strains level revealed that one of the strains was SB0133 while the other strain was new and had not been reported to the M. bovis database prior to this study. Hence, it has now been reported to the database, and designated as SB1953. In conclusion, the results of the present study have shown that the majority of camel TB lesions are caused by mycobacteria other than Mycobacterium tuberculosis complex. And hence further identification and characterization of these species would be useful towards the efforts made to control TB in camels.

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Section: Discussionsupporting

confidence: 86%

Pathology of Camel Tuberculosis and Molecular Characterization of Its Causative Agents in Pastoral Regions of Ethiopia

et al. 2011

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“…The overall prevalence of bTB from gross suspected TB lesion(s) in the present study was 2.78% which is comparable to 2.7% reported by Bekele and Belay [10], but lower than other findings ranging from 3.5% to 10.2% [7–9, 11–15, 25]. These variations could be explained by many factors including differences in the disease status in the animal populations, the sample size and the type of production system from where the slaughtered cattle were originated.…”

Section: Discussionsupporting

confidence: 78%

“…Other Ethiopian studies [7–15] undertaken at abattoirs have reported bTB in cattle based on TB-like lesion with an estimated average prevalence of 5.57%. In addition, M. bovis was also recovered from TB lesions in cattle, spoligotyped, and their strain types were identified and reported by previous studies in Ethiopia [7, 9, 16–19]. Infection with M. bovis can be transmitted from cattle to humans, mainly through the consumption of contaminated milk and meat [20], although there is no evidence that this has happened in Ethiopia, where raw milk and meat consumption is widely habituated.…”

Section: Introductionmentioning

confidence: 96%

Nontuberculosis mycobacteria are the major causes of tuberculosis like lesions in cattle slaughtered at Bahir Dar Abattoir, northwestern Ethiopia

Nuru¹,

Zewude²,

Mohammed³

et al. 2017

BMC Vet Res

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BackgroundThe main cause of bovine tuberculosis (bTB) is believed to be Mycobacterium bovis (M. bovis). Nontuberculosis mycobacteria (NTM) are neglected but opportunistic pathogens and obstacles for bTB diagnosis. This study aimed to isolate and characterize the mycobacteria organisms involved in causing TB-like lesions in cattle in northwestern Ethiopia.ResultsA total of 2846 carcasses of cattle were inspected for TB lesions. Ninety six tissues (including lymph nodes such as submandibular, retropharyngeal, tonsilar, mediatinal, bronchial and mesenteric, and organs such as lung, liver and kidney) with suspicious TB lesion(s) were collected and cultured on Lowenstein-Jensen medium. Twenty one showed culture growth, of which only 17 were identified containing acid fast bacilli (AFB) by Ziehl–Neelsen staining. Among the 17 AFB isolates 15 generated a polymerase chain reaction product of 1030 bp by gel electrophoresis based on the 16S ribosomal RNA gene amplification. No M. tuberculosis complex species were isolated. Further characterization by Genotype Mycobacterium CM assay showed 6 isolates identified as M. peregrinum. Eight isolates represented by mixed species, which includes M. fortuitum-peregrinum (3 isolates), M. gordonae-peregrinum (3 isolates) and M. fortuitum-gordonae-peregrinum (2 isolates). One NTM could not be interpreted.ConclusionA significant number of NTM species were isolated from TB-like lesions of grazing cattle slaughtered at Bahir Dar Abattoir. Such finding could suggest the role of NTM in causing lesions in cattle. Further investigations are recommended on the pathogenesis of the reported NTM species in cattle, and if they have public health significance.

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“…Such methods are also expensive, time consuming, laborious and not practical for surveillance purposes (Strong and Kubica 1985;Grange et al 1996;Parsons et al 2002;Warren et al 2006). Further, the interpretation of results is highly subjective and prone to errors such as those possible when interpreting differences in colony morphology (Strong and Kubica 1985;Grange et al 1996;Ameni et al 2010). Though also prone to inspector subjectivities and errors, meat inspection provides very significant insight into the prevalence of many infectious diseases and plays vital roles in both the quality assurance and quality control systems for the gross inspection of carcasses (Edwards et al 1997;Hinton and Green 1997;Asseged et al 2004).…”

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confidence: 99%

Prevalence of bovine tuberculosis in cattle in the highlands of Cameroon based on the detection of lesions in slaughtered cattle and tuberculin skin tests of live cattle

Awah-Ndukum¹,

Kudi²,

Bradley³

et al. 2012

Vet. Med. - Czech

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Bovine tuberculosis (TB) is an important neglected zoonosis in Cameroon, where many communities depend on their livestock for livelihood and the incidence of human TB and TB-HIV/AIDS co-infection are high and increasing annually. The aim of this study was to estimate the prevalence of bovine TB in cattle in the highlands of Cameroon. The magnitude and trend of detecting TB lesions in slaughtered cattle (1994 to 2010) and tuberculin skin tests (TST) in 2853 cattle (84 herds) of 39 livestock rearing communities were analysed. Of 129 165 slaughtered cattle inspected, 599 (0.46%; 95% CI: 0.43%-0.50%) showed suspected TB lesions among a total of 983 (0.76%; 95% CI: 0.71%-0.81%) identified pathologies. The monthly TB detection rates ranged from 0.30% (95% CI: 0.20%-0.40%) to 0.81% (95% CI: 0.64%-0.98%) and annual rates from 0.04% (95% CI: 0%-0.11%) to 1.46% (95% CI: 1.22%-1.69%). The rates were not affected (P < 0.05) by season and fluctuating peaks were also recorded. The comparative TST revealed that bovine TB was widely distributed in live cattle (4.67%; 95% CI: 3.89%-5.44%) and was higher (χ 2 = 17.50, P ≤ 0.001) in the Western highlands than Adamawa plateaux.Comparative TST bovine TB reactors were higher (P < 0.05) in cattle managed in semi-intensive and beef production systems compared to the others. Animals in small herds showed higher (χ 2 = 4.283, P = 0.038) rates than those in large herds. Bovine TB prevalence in exotic/upgraded cattle was comparable to that of the Red Bororo zebu but higher than the rates in Guadali (χ 2 = 4.971, P = 0.026) and White Fulani (χ 2 = 5.6, P = 0.018) zebus.Among the indigenous zebus, the rate was higher in Red Bororo than the Guadali (χ 2 = 6.244, P = 0.012) and White Fulani (χ 2 = 6.568, P = 0.010). Sex did not influence (χ 2 = 0.410, P = 0.522) bovine TB prevalence in this study but diagnosis of the disease was higher (χ 2 = 5.787; P = 0.016) among adult/older cattle than in younger animals. Further analysis of the TST responses revealed that atypical mycobacterial infections was widespread and 6.83% of tested animals showed positive reactions at both bovine and avian tuberculin injection sites and a strong association (χ 2 = 2.512; P = 0.113) between skin responses to both tuberculins. The study confirms that bovine TB is prevalent in live cattle and meat production abattoirs in Cameroon and we recommend strict a interpretation of TST results for maximum diagnosis of the disease in the local environment. A need for comprehensive investigation of the molecular epidemiology, zoonotic risks and the public health importance of bovine TB in Cameroon cannot be overemphasised.

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Molecular typing of Mycobacterium bovis isolated from tuberculosis lesions of cattle in north eastern Ethiopia

Cited by 46 publications

References 29 publications

Pathology of Camel Tuberculosis and Molecular Characterization of Its Causative Agents in Pastoral Regions of Ethiopia

Pathology of Camel Tuberculosis and Molecular Characterization of Its Causative Agents in Pastoral Regions of Ethiopia

Nontuberculosis mycobacteria are the major causes of tuberculosis like lesions in cattle slaughtered at Bahir Dar Abattoir, northwestern Ethiopia

Prevalence of bovine tuberculosis in cattle in the highlands of Cameroon based on the detection of lesions in slaughtered cattle and tuberculin skin tests of live cattle

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