Molecular typing of industrial strains of Pseudomonas spp. isolated from milk and genetical and biochemical characterization of an extracellular protease produced by one of them

Quantitative and qualitative variability of the caseinolytic potential of different strains of Pseudomonas fluorescens: Implications for the stability of casein micelles of UHT milks during their storage

“…The differences in proteolytic activity between the strain F and the strains CIP 7325 and CIP 6913 were also observed by Dufour et al (2008). These authors determined activity using azocasein as substrate and reported that the first strain was highly proteolytic although the activities of the two others were relatively weak.…”

“…These strains had been selected because of their differences in term of proteolytic activity quantified using azocasein as substrate, as described by Dufour et al (2008) (results not shown). By casein zymography, the presence of one electrophoretic band at about 50 kDa was detected suggesting the potential presence of an extracellular protease for all strains (results not shown).…”

Proteolysis of casein micelles by Pseudomonas fluorescens CNRZ 798 contributes to the destabilisation of UHT milk during its storage

“…The caseinolytic potential of these proteases is highly variable and strain dependent (Dufour et al 2008). Some studies have been performed to understand the proteolytic activities on the milk proteins and especially on the casein molecules.…”

Detection of Proteolysis in Milk by Pseudomonas fluorescens Using Urea PAGE Method

“…It was stipulated that whole casein could have a configuration that made it more susceptible to enzyme action (Mitchell and Marshall, 1989). The caseinolytic potential of these proteases is highly variable and strain dependent (Dufour et al, 2008). Some studies have been performed to understand the proteolytic activities on the milk proteins and especially on the casein molecules.…”