Molecular Typing of Pneumococci for Investigation of Linked Cases of Invasive Pneumococcal Disease

Pichon, Bruno; Moyce, Laura J; Sheppard, Carmen; Slack, Mary; Turbitt, Deborah; Pebody, Richard; Spencer, David A.; Edwards, Justin; Krahé, D.; George, Robert C.

doi:10.1128/jcm.02054-09

Cited by 13 publications

(21 citation statements)

References 10 publications

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“…An MLVA scheme has been developed for S. pneumoniae that scores electrophoretically-determined size variation in tandem repeat arrays from 16 loci (Koeck et al, 2005). This MLVA scheme has been useful for confirming relatedness of pneumococcal outbreak isolates of serotypes 1 (Yaro et al, 2006) and 5 (Pichon et al, 2010). However, use of MLVA to further elucidate population structure can be limited by the independent evolution of the same repeat array sizes in different strains (i.e.…”

Section: Introductionmentioning

confidence: 99%

Population structure of hyperinvasive serotype 12F, clonal complex 218 Streptococcus pneumoniae revealed by multilocus boxB sequence typing

Rakov¹,

Ubukata²,

Robinson³

2011

Infection, Genetics and Evolution

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At least four outbreaks of invasive disease caused by serotype 12F, clonal complex 218 Streptococcus pneumoniae have occurred in the United States over the past two decades. We studied the population structure of this clonal complex using a sample of 203 outbreak and surveillance isolates that were collected over 22 years from 34 US states and eight other countries. Conventional multilocus sequence typing identified five types and distinguished a single outbreak from the others. To improve typing resolution, multilocus boxB sequence typing (MLBT) was developed from 10 variable boxB minisatellite loci. MLBT identified 86 types and distinguished between each of the four outbreaks. Diversity across boxB loci tended to be positively correlated with repeat array size and, overall, best fit the infinite alleles mutation model. Multilocus linkage disequilibrium was strong, but pairwise disequilibrium decreased with the physical distance between loci and was strongest in one large region of the chromosome, indicating recent recombinations. Two major clusters were identified in the sample, and they were differentiated geographically, as western and more easterly US clusters, and temporally, as clusters that predominated before and after the licensure of pneumococcal conjugate vaccines. The diversity and linkage disequilibrium within these two clusters also differed, suggesting different population dynamics. MLBT revealed hidden aspects of the population structure of these hyperinvasive pneumococci, and it may provide a useful adjunct tool for outbreak investigations, surveillance, and population genetics studies of other pneumococcal clonal complexes.

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Section: Introductionmentioning

confidence: 99%

Population structure of hyperinvasive serotype 12F, clonal complex 218 Streptococcus pneumoniae revealed by multilocus boxB sequence typing

Rakov¹,

Ubukata²,

Robinson³

2011

Infection, Genetics and Evolution

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“…In the first description, the Colombia 5 - ST289 clone showed resistance to co-trimoxazole, tetracycline, and chloramphenicol (26). This Ser5 clone has been previously found as a cause of IPD in children from Israel (25) and in outbreaks in Canada and the United Kingdom (9,12). However, in Israel, the ST289 clone was susceptible to all antimicrobials studied, with the exception of co-trimoxazole (36% resistant) (25).…”

Section: Discussionmentioning

confidence: 95%

“…Ser5 pneumococci have been associated with chains of infection, such as those described in Israel (11), the United Kingdom (12), and Canada (9,13,14). Outbreaks of pneumococcal infections have been documented and occur typically in closed communities within institutions, and they tend to be caused by a limited number of pneumococcal serotypes.…”

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confidence: 99%

Serotype 5 Pneumococci Causing Invasive Pneumococcal Disease Outbreaks in Barcelona, Spain (1997 to 2011)

et al. 2013

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fIn this study, we analyzed the clinical and molecular epidemiology of invasive serotype 5 (Ser5) pneumococcal isolates in four teaching hospitals in the Barcelona, Spain, area (from 1997 to 2011). Among 5,093 invasive pneumococcal isolates collected, 134 (2.6%) Ser5 isolates were detected. Although the overall incidence of Ser5-related invasive pneumococcal disease (IPD) was low (0.25 cases/100,000 inhabitants), three incidence peaks were detected: 0.63/100,000 in 1999, 1.15/100,000 in 2005, and 0.37/ 100,000 in 2009. The rates of Ser5 IPD were higher among young adults (18 to 64 years old) and older adults (>64 years old) in the first two peaks, whereas they were higher among children in 2009. The majority (88.8%) of the patients presented with pneumonia. Comorbid conditions were present in young adults (47.6%) and older adults (78.7%), the most common comorbid conditions being chronic obstructive pulmonary disease (20.6% and 38.3%, respectively) and cardiovascular diseases (11.1% and 38.3%, respectively). The mortality rates were higher among older adults (8.5%). All Ser5 pneumococci tested were fully susceptible to penicillin, cefotaxime, erythromycin, and ciprofloxacin. The resistance rates were 48.5% for co-trimoxazole, 6.7% for chloramphenicol, and 6% for tetracycline. Two major related sequence types (STs), ST1223 (n ‫؍‬ 65) and ST289 (n ‫؍‬ 61), were detected. The

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“…Although deaths from pneumonia in children in the UK are rare, there is an increasing observation of empyema and complicated pneumonia developing 53. The explanation for this is not as yet clear but could be related to the changing pneumococcal serotype prevalence54 which was expanded on by Professor D Goldblatt (London). Professor Goldblatt's talk on the pneumococcal vaccines centred on the introduction of new pneumococcal conjugate vaccines and how these have led to a reduction in the incidence of invasive pneumococcal disease.…”

Section: Respiratory Infectionsmentioning

confidence: 99%

A review of the British Thoracic Society scientific meeting 1-3 December 2010

Church

Bayes²,

Fisher³

2011

Thorax

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This review presents the inaugural report of the British Thoracic Society Winter Scientific Meeting held from 1 st e3rd December 2010. Although a wide spectrum of respiratory research was presented at the meeting the content of the review focuses specifically on three key themes of inflammation and lung injury, airways disease and respiratory infection. Advances in both clinical and translational respiratory research presented within the major symposia and spoken sessions related to these themes are summarised.

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Molecular Typing of Pneumococci for Investigation of Linked Cases of Invasive Pneumococcal Disease

Cited by 13 publications

References 10 publications

Population structure of hyperinvasive serotype 12F, clonal complex 218 Streptococcus pneumoniae revealed by multilocus boxB sequence typing

Population structure of hyperinvasive serotype 12F, clonal complex 218 Streptococcus pneumoniae revealed by multilocus boxB sequence typing

Serotype 5 Pneumococci Causing Invasive Pneumococcal Disease Outbreaks in Barcelona, Spain (1997 to 2011)

A review of the British Thoracic Society scientific meeting 1-3 December 2010

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