Molecular typing of Shigella sonnei isolates circulating in Nanjing, China, 2007–2011

Cheng, Tingting; Shi, Xiaochao; Wei, Yong; Wang, Jianping; Xie, Gary; Ding, Jie

doi:10.3855/jidc.4933

Cited by 13 publications

(11 citation statements)

References 20 publications

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“…Compared with the MLST pro les, MLVA and PFGE may be forceful tools that can provide a satisfactory level of discrimination. Whereas the function of MLVA in phylogenetic analysis of different bacterial species or serotypes are poorly targeted [37]. Nevertheless, MLVA is an ordinarily used typing tool that has been used for establishing genetic relatedness and performing phylogenetic analysis among strains of monomorphic species.…”

Section: Discussionmentioning

confidence: 99%

Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea

Zhu

Wang

Cao

et al. 2020

Preprint

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Background The natural hosts of Shigella are conventionally humans and other primates; however, the host range of Shigella has been shown to expand to many animals. Although Shigella is becoming a huge threat to animals, there is limited information on the genetic background of local strains. The purpose of this study was to assess the presence of virulence factors and the molecular characteristics of S. flexneri isolated from calves with diarrhea. Results Fifty-four S. flexneri isolates possessed four typical biochemical characteristics of Shigella. The prevalences of ipaH, virA, ipaBCD, ial, sen, set1A, set1B and stx were 100%, 100%, 77.78%, 79.63%, 48.15%, 48.15% and 0, respectively. MLVA based on 8 VNTR loci discriminated the isolates into 39 different MTs, PFGE based on NotI digestion divided the 54 isolates into 31 PTs, and MLST based on 15 housekeeping genes differentiated the isolates into 7 STs. Conclusion Our findings of this study have enriched our knowledge of the molecular characteristics of S. flexneri collected from diarrheal calves, which will be important for addressing clinical and epidemiological issues regarding Shigellosis.

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Section: Discussionmentioning

confidence: 99%

Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea

Zhu

Wang

Cao

et al. 2020

Preprint

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“…According to Cheng et al . , almost 100% of the S. sonnei strains isolated between 2007 and 2011 in Nanjing in China were typed as ST114 by the MLST method amplifying 15 housekeeping genes. Therefore, S. sonnei strains have been shown to have descended from a common precursor and belong to the same clonal complex.…”

Section: Discussionmentioning

confidence: 99%

Molecular typing and occurrence of beta‐lactam resistance genes of Shigella sonnei strains isolated from 1983 to 2014 in the São Paulo state of Brazil

Seribelli

Frazão

Medeiros

et al. 2017

Microbiology and Immunology

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Shigella sonnei, which has generally been associated with dysentery in developed countries, has recently been emerging in developing countries. Specifically, in Brazil few published studies have that molecularly characterized this species. The aims of this study were to analyze the efficacy of typing using multiple-locus variable-number tandem-repeat analysis (MLVA), study the phylogeny by multi-locus sequence typing (MLST) and assess the presence of some beta-lactam resistance genes in S. sonnei strains isolated from human diarrhoeic faeces in the São Paulo State in Brazil between 1983 and 2014. Seventy-two such S. sonnei strains were typed by MLVA and grouped into two clusters. The discrimination index of MLVA was found to be 0.996. Twenty strains were typed by MLST as ST152. In addition, the bla gene was detected in eight (72.7%) of the 11 S. sonnei strains that had previously been shown to be resistant to β-lactams. However, bla , bla and bla genes were not found. MLVA results suggested the existence of two prevalent subtypes in the S. sonnei strains studied, confirming previous results. Moreover, MLVA efficiently discriminated monomorphic S. sonnei species. Because the S. sonnei strains studied belonged to clonal complex 152 and all isolates were typed as ST152, MLST is not a suitable method for studying the population structure of S. sonnei. Although, the rates of β-lactam resistance were not high in the present study, the frequency of bla may represent a risk for patients receiving antimicrobial treatment. Taken together, the results provide better molecular characterization of this globally clinically important pathogen.

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“…The dentrogram of all the 76 isolates showed a 65% coefficient of similarity, which shared similar discriminatory power with S . sonnei isolates from other provinces [ 29 , 30 ] and other countries [ 3 , 31 ]. Isolates from close years were almost clustered closely and further divided into sub-clusters based on the region of isolation ( Fig 1 ), indicating the genetic diversity and the genetic changes over the timescales.…”

Section: Discussionmentioning

confidence: 99%

“…MLVA is a prominent typing tool which has been developed for S . sonnei [ 2 , 3 , 15 , 30 ]. In the present study, the 76 S .…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Shigella sonnei: An Increasingly Prevalent Etiologic Agent of Shigellosis in Guizhou Province, Southwest of China

Wang²,

Wei

et al. 2016

PLoS ONE

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BackgroundShigellosis is a serious problem in Guizhou and Shigella sonnei is an increasingly prevalent etiologic agent of local shigellosis cases. No data, however, are available about the molecular characterization of the local isolates of S. sonnei. We have conducted this study to molecularly characterize the clinical isolates of S. sonnei in Guizhou Province.Results76 S. sonnei isolates, including four isolates from 1974–1982 and 72 isolates from 2008–2010, were used for analysis in this study. Pulsed-field gel electrophoresis (PFGE) based on XbaI digestion divided the 76 isolates into 38 PFGE patterns (PT) and 15 PTs were represented by more than one isolates with PT31 (N = 8) containing the most number of isolates, followed with PT2 (N = 6). Multiple-Locus Variable number tandem repeat (VNTR) Analysis (MLVA) based on seven VNTR loci discriminated them into 19 different MLVA types (MTs), and four MTs were represented by more than one isolate with MT4 (N = 39) containing the most number of isolates, followed with MT12 (N = 18). 15 Multilocus sequence typing (MLST) base on 15 loci differentiated the isolates into six sequence types (STs), among which four STs were novel. The most common STs are ST76 (N = 43) and ST116 (N = 25), accounting for 92.1%. Correlation between genetic relationships and geographical origins or isolation years was observed among the isolates studied. Majority of isolates were clustered in accordance with the origin of isolation years based on the genetic data, which were also from similar geographical origins.ConclusionsOur results revealed the molecular characteristics including the specific genotypes such as four novel STs, clonal relationship, and genetic changes of local isolates from different years, which enhances our understanding of molecular characteristics of S. sonnei and contributes to the prevention and control of shigellosis in Guizhou Province.

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Molecular typing of Shigella sonnei isolates circulating in Nanjing, China, 2007–2011

Cited by 13 publications

References 20 publications

Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea

Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea

Molecular typing and occurrence of beta‐lactam resistance genes of Shigella sonnei strains isolated from 1983 to 2014 in the São Paulo state of Brazil

Molecular Characterization of Shigella sonnei: An Increasingly Prevalent Etiologic Agent of Shigellosis in Guizhou Province, Southwest of China

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