Molecular Typing of Treponema pallidum

Tian, Haijun; Li, Zhen; Li, Zhongwei; Hou, Jin; Zheng, Ruisheng; Li, Furong; Liu, Ruyuan; Liu, Bing; Zhang, Furen

doi:10.1097/olq.0000000000000155

Cited by 18 publications

(5 citation statements)

References 4 publications

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“…Rabbit passed strains BAL9 was included as representative for type “H”, which was not found among clinical samples yet. For subtype”L” the number of identified samples is not known [ 37 ]. Subtype”J” does not correspond to TPA but was found in T .…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of Treponema pallidum subsp. pallidum in Switzerland and France with a new multilocus sequence typing scheme

et al. 2018

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Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.

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Section: Resultsmentioning

confidence: 99%

Molecular characterization of Treponema pallidum subsp. pallidum in Switzerland and France with a new multilocus sequence typing scheme

et al. 2018

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“…In this study, the most prevalent genotypes were 14d/d and 14d/f (15% each). Subtype 14d/f was also a predominant genotype in other countries including China [ 12 , 23 , 25 , 27 , 32 ], Peru [ 21 ], Russia [ 29 ], and the USA [ 12 , 24 ]. On the other hand, subtype 14d/d was never observed to be a predominant genotype in any country where ECDCT was used [ 12 , 21 – 32 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance

et al. 2017

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A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequencing-based molecular typing (SBMT) and Enhanced CDC-typing (ECDCT). By SBMT, 10 different Treponema pallidum subsp. pallidum (TPA) genotypes were found, of which six were related to the TPA SS14 strain, and four to the TPA Nichols strain. The 23S rRNA gene was amplified in samples isolated from 42 patients, and in six of them (14.3%), either the A2058G (four patients, 9.5%) or the A2059G (two patients, 4.8%) mutations were found. In addition to Taiwan, Madagascar and Peru, Argentina is another country where the prevalence of Nichols-like isolates (26.8%) is greater than 10%.

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“…The correspondence by Mikalova et a l. 1 in this issue of Sexually Transmitted Diseases makes the important and very interesting observation that the genital lesion of a man diagnosed as having primary syphilis in Paris, France 2 , contained a Treponema strain that displays several molecular signatures that suggest infection not with Treponema pallidum subsp. pallidum as expected, but with T. pallidum subsp.…”

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confidence: 99%