Molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in Belgrade, Serbia

Jovanović, M.; Milošević, Branko; Tošić, Tanja; Stevanović, Goran; Mioljević, Vesna; Inđić, Nikola; Velebit, Branko; Zervos, Marcus J.

doi:10.1556/030.62.2015.2.5

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…In the past, the main threat related to enterococci antibiotic resistance was the circulation of VRE among humans and animals [26]. Currently, on the basis of more recent studies and our results, VRE seem to be not frequent in pet population, but other concerns have been added: the increasing spread of enterococci resistant to several other antimicrobials.…”

Section: Resultsmentioning

confidence: 65%

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs

Bertelloni

Salvadori

Lotti

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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Enterococci are opportunistic bacteria that cause severe infections in animals and humans, capable to acquire, express, and transfer antimicrobial resistance. Susceptibility to 21 antimicrobial agents was tested by the disk diffusion method in 222 Enterococcus spp. strains isolated from the fecal samples of 287 healthy domestic dogs. Vancomycin and ampicillin minimum inhibitory concentrations (MICs) and high-level aminoglycoside resistance (HLAR) tests were also performed. Isolates showed resistance mainly to streptomycin (88.7%), neomycin (80.6%), and tetracycline (69.4%). Forty-two (18.9%) isolates showed an HLAR to streptomycin and 15 (6.7%) to gentamicin. Vancomycin and ampicillin MIC values showed 1 and 18 resistant strains, respectively. One hundred and thirty-six (61.2%) strains were classified as multidrug resistant and six (2.7%) strains as possibly extensively drug-resistant bacteria. Enterococcus faecium and Enterococcus faecalis were the most prevalent antimicrobial resistant species. Companion animals, which often live in close contact with their owners and share the same environment, represent a serious source of enterococci resistant to several antibiotics; for this reason, they may be a hazard for public health by providing a conduit for the entrance of resistance genes into the community.

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Section: Resultsmentioning

confidence: 65%

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs

Bertelloni

Salvadori

Lotti

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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“…When interpreting the MIC/disk diffusion results, it is important to ensure that isolate is not E. casselifalvus or E. gallinarum, species that possess intrinsic resistance to glycopeptides. Furthermore, despite the number of studies on antibiotic-resistance in enterococci from Serbian clinical settings, there were no data about prevalence of VRE in the outpatients' settings in our country [18,19,20]. The results of the Central Asian and Eastern European Surveillance of Antimicrobial Resistance (CAESAR network) [7] showed high level of resistance to aminopenicillins among E. faecalis and E. faecium (41% and 94%, respectively).…”

Section: Discussionmentioning

confidence: 95%

The phenotypic and genotypic characterization of vancomycin-resistant enterococci in outpatients’ urine culture

et al. 2017

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Introduction/Objective In the era of emerging antibacterial resistance, the major burden of resistant strains is on hospitalized patients. Although community factors are also important in the spread of resistance, less attention has been paid to non-healthcare settings. The aim of the study is to determine the prevalence of vancomycin-resistant enterococci (VRE) in the outpatient's urine culture and to perform phenotypic and genotypic characterization of VRE strains. Methods During an 18-month period, a total of 5,164 Enterococcus spp. strains were isolated from urine and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antimicrobial susceptibility testing was performed by disk diffusion method and by gradient test for glycopeptideresistant strains. Genotypic characterization of VRE strains was done by multiplex polymerase chain reaction for the detection of the vancomycin resistance genes. Results Among the isolated enterococci, 5,060 (98%) were E. faecalis and 104 (2%) were E. faecium. E. faecalis strains were susceptible to all tested antibiotics except norfloxacin (33% of strains were resistant), while E. faecium showed high level of resistance to most of the tested agents (91.3% to ampicillin, 77% to norfloxacin, and 75% to nitrofurantoin), and 26% of strains were resistant to vancomycin and teicoplanin. VanA gene was detected in all vancomycin resistant E. faecium (VREfm) strains. Conclusion A high proportion of VREfm was noticed among outpatients in our country. All analyzed VREfm strains belonged to vanA genotype. Future surveillance studies of VRE are needed to follow up on this baseline study to monitor any possible changes in abundance and genotype of VRE in this population group.

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First nosocomial outbreak of vanA-type vancomycin-resistant Enterococcus raffinosus in France

Jolivet

Fines-Guyon

Nebbad

et al. 2016

Journal of Hospital Infection

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Molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in Belgrade, Serbia

Cited by 3 publications

References 15 publications

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs

The phenotypic and genotypic characterization of vancomycin-resistant enterococci in outpatients’ urine culture

First nosocomial outbreak of vanA-type vancomycin-resistant Enterococcus raffinosus in France

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