Molecular weight dependence of calcification of polyethylene glycol hydrogels

Hossainy, Sahir; Hubbell, Jeffrey A.

doi:10.1016/0142-9612(94)90118-x

Cited by 38 publications

(17 citation statements)

References 11 publications

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“…There is growing recognition, however, that while the TCPS culture platform allows for facile experiment implementation, the extremely stiff modulus causes cells to respond in a potentially non-physiologically relevant manner [43]. To address this issue, several alternative culture platforms, both natural [3,12,19], and synthetic [7,10,14,23,25,27,33–37,42], have been developed. Here, the facile manipulation and tailorability of PEG hydrogels has been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…These materials were fabricated by reacting norbornene functionalized 8-armed PEG monomers with cysteine (i.e., thiol) functionalized small peptides in the presence of UV light and a photoinitiator. Through facile selection of the macromolecular precursors, the substrate stiffness can be manipulated by altering the molecular weight of poly(ethylene glycol) (PEG) monomers for large changes in gel modulus or performing the reaction off stoichiometry for small modulus adjustments [20–23]. The biochemical composition of the gel is also easily tailored to the hypothesis at hand, by simply introducing cysteine-containing peptides that are covalently bound within the gel network based on the initial reactant stoichiometry.…”

Section: Introductionmentioning

confidence: 99%

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Small peptide functionalized thiol–ene hydrogels as culture substrates for understanding valvular interstitial cell activation and de novo tissue deposition

2012

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A thiol-ene polymerization platform was used to synthesize peptide functionalized poly(ethylene glycol) (PEG) hydrogels, which were initially characterized and compared to theoretical predictions of Young’s modulus via a theoretical crosslinking density equation presented herein. After thorough characterization, this material system’s utility for answering specific biological hypotheses was demonstrated with the culture and observation of aortic valvular interstitial cells (VICs). Specifically, these materials were used to better understand the role of substrate elasticity and biochemical functionality on VIC α-smooth muscle (αSMA) expression and secretory properties (i.e., de novo ECM). The Young’s moduli of the hydrogels were varied from 28kPa (activating, 90% myofibroblasts) to 4kPa (non-activating, 15% myofibroblast) substrates, and the biochemical functionality was tailored by incorporating three small adhesive peptide sequences, RGDS, VGVAPG, and P15. To promote VIC adhesion, a basal [RGDS] of 0.8mM was used in all formulations, while the [VGVAPG] or [P15] were varied to be lower, equal, or higher than 0.8mM. The substrates with 1.2mM VGVAPG and all gels with P15 led to significantly higher αSMA expression for both stiff and soft substrates, as compared to 0.8mM RGDS alone. Importantly, all gel conditions were significantly lower than TCPS (~4–10 fold difference). The ECM produced significantly decreased as the total integrin binding peptide concentration increased, but was significantly higher than that expressed on TCPS. This easily tailored material system provides a useful culture platform to improve the fundamental understanding of VIC biology through isolating specific biological cues and observing VIC function.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Small peptide functionalized thiol–ene hydrogels as culture substrates for understanding valvular interstitial cell activation and de novo tissue deposition

2012

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“…[32] Others have reported potential issues with protein adsorption to low-molecular-weight PEG gels. [33,34] However, hydrogels with bulk compressive moduli in the range of 10±100 kPa may be prepared from higher molecular weight PEGs, suggesting that this templating approach could be readily applied to such gels. [35,36] As illustrated in Figure 2a, mouse NR6 fibroblasts were able to attach and spread inside RGD-modified scaffolds over at least several days when cultured within scaffolds in vitro.…”

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confidence: 99%

Bioactive Hydrogels with an Ordered Cellular Structure Combine Interconnected Macroporosity and Robust Mechanical Properties

et al. 2005

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A method for preparing porous hydrogel scaffolds suitable for bioengineering applications based on colloidal‐crystal templating is demonstrated. Templated scaffolds are shown to have highly interconnected porosities at moderate void fractions due to their ordered arrangement of voids, and thus combine abundant pathways for cell migration (see Figure) and mass transport with compressive moduli comparable to soft tissues.

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“…Free radicals generated by UV irradiation of initiators for a given time (2.5 min) would attack the vinyl groups at the ends of DA-Pluronic polymers, followed by conversion into reactive radicals. The reactions between reactive Pluronic polymers and free radicals resulted in a chemically crosslinked hydrogel state [20,35,43,44]. The interaction between heparin and Pluronic micelles also affected the photo-polymerization of DA-PF 127 solution (Fig.…”

Section: Heparin Effect On the Photo-polymerization Of Da-pf 127mentioning

confidence: 92%

Accelerated Micellization and Aggregation of Pluronic Micelles by Interaction with Heparin

Lee

Tae

Kim

2010

Journal of Biomaterials Science, Polymer Edition

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The effect of attractive interaction between heparin and Pluronic co-polymer, two important macromolecules in biomedical applications, on the micellar state of Pluronic co-polymer was characterized. By the addition of heparin, the critical micellization temperature (CMT) of Pluronic solution decreased, indicating that heparin promoted micellization. Also, the Pluronic micelles were associated by the action of heparin, and the degree of aggregation became more pronounced by increasing the heparin amount. The association among Pluronic micelles by heparin enhanced the efficiency of chemical cross-linking of di-acrylated Pluronic solution by photo-polymerization, as well as the physical gelation by micelle packing. Thus, heparin can act as a sensitive modulator of Pluronic micelles in addition to its biological functions.

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Molecular weight dependence of calcification of polyethylene glycol hydrogels

Cited by 38 publications

References 11 publications

Small peptide functionalized thiol–ene hydrogels as culture substrates for understanding valvular interstitial cell activation and de novo tissue deposition

Small peptide functionalized thiol–ene hydrogels as culture substrates for understanding valvular interstitial cell activation and de novo tissue deposition

Bioactive Hydrogels with an Ordered Cellular Structure Combine Interconnected Macroporosity and Robust Mechanical Properties

Accelerated Micellization and Aggregation of Pluronic Micelles by Interaction with Heparin

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