Detection of the virus is the primary factor to discover
and block
the occurrence and development of the virus epidemic. Here, an ultrasensitive
paper-based virus molecular imprinting sensor is developed to detect
two viruses simultaneously in which the detection limit of the influenza
virus (H5N1) is 16.0 aM (9.63 × 103 particles/mL)
while that of the Hepatitis B Virus (HBV) is 129 fM (7.77 × 107 particles/mL). This paper-based sensor is low cost and is
easy to cut, store, and carry. In addition, the visual semiquantitative
detection of two viruses is achieved by using two aptamer-functionalized
persistent luminescent nanoparticles as signal probes. These probes
and the imprinted cavities on the paper-based material formed sandwich-type
double recognition of the target viruses. This sensor has extremely
high sensitivity to the H5N1 virus, which is of great value to solve
the influenza epidemic with the most outbreaks in history, and also
opens up a new way for the prevention and control of other virus epidemics.
This cheap and portable visual sensor provides the possibility for
self-service detection and can greatly reduce the pressure on medical
staff and reduce the risk of virus infection caused by the concentration
of people to be tested.