Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples

Abstract

An important challenge for scientific research is the production of artificial systems able to mimic the recognition mechanisms occurring at the molecular level in living systems. A valid contribution in this direction resulted from the development of molecular imprinting. In this work, a novel molecularly imprinted polymer composite membrane (MIM) was synthesized and employed for the selective detection in urine samples of 2-deoxyadenosine (2-dA), an important tumoral marker. By thermal polymerization, the 2-dA-MIM was cross-linked on the surface of a polyvinylidene-difluoride (PVDF) membrane. By characterization techniques, the linking of the imprinted polymer on the surface of the membrane was found. Batch-wise guest binding experiments confirmed the absorption capacity of the synthesized membrane towards the template molecule. Subsequently, a time-course of 2-dA retention on membrane was performed and the best minimum time (30 min) to bind the molecule was established. HPLC analysis was also performed to carry out a rapid detection of target molecule in urine sample with a recovery capacity of 85%. The experiments indicated that the MIM was highly selective and can be used for revealing the presence of 2-dA in urine samples.

Stainless Steel Wire Mesh Supported Molecularly Imprinted Composite Membranes for Selective Separation of Ebracteolata Compound B from Euphorbia fischeriana

“…Various types of molecularly imprinted materials with large surface areas have been synthesized, especially with respect to molecularly imprinted polymer membranes being used as separation tools, which can selectively transport a target molecule from a mixture solution by permeating through the thin membrane [21,22,23,24,25]. Molecularly imprinted composite membranes with the ultrafiltration or microfiltration supports are thinner, more flexible, and higher flux compared with the other freestanding membranes [26,27,28,29,30,31]. Selecting an appropriate support and functionalizing the support surface are the keys to obtaining high-quality membrane properties [32].…”

Urinary l‐kynurenine quantification and selective extraction through a molecularly imprinted solid‐phase extraction device

“…In this study, we attempted to develop a system that prevents time‐consuming pretreatments that require highly specialized operators. In particular, starting from the experience of our research group , molecular imprinting technology (MIT) was chosen to obtain a polymer selective for l ‐Kyn to be employed as a sorbent material in an SPE device to extract and purify endogenous l ‐Kyn before HPLC analysis. Thus, the matrix effect could be greatly reduced and the use of MS detector could be substituted with a less expensive and more accessible detection system such as a diode array detector (DAD).…”

Molecularly Imprinted Tio2 Inorganic Film and PVDF/Tio2 Composite Film as Sensors for the Detection of Chemical Threat Agents Using Quartz Crystal Microbalance

“…Resonance frequency of the quartz based sensor with applied voltage is sensitive to mass change. Binding of target analyte molecules onto the molecular cavities on the MIP would lead to an increase in mass which is reflected by a proportional drop in its resonance frequency [9,10,11]. The relationship between change of resonance frequency and change of mass for a QCM sensor could be described by the Sauerbrey equation (eq.…”