Molecularly Imprinted Poly‐o‐phenylenediamine Electrochemical Sensor for Entacapone

Abstract

We have developed a molecularly imprinted polymer (MIP) electrochemical sensor for entacapone (ETC) based on an electropolymerised polyphenylenediamine (Po‐PD) on a glassy carbon electrode (GCE) surface. The direct electropolymerisation of the o‐phenylenediamine monomer (o‐PD) was carried out with ETC as a template. The steps of electropolymerization process, template removal and binding of the analyte were tested by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) using [Fe(CN)6]3−/[Fe(CN)6]4 − as a redox probe. The operation of the sensor has been investigated by differential pulse voltammetry (DPV). Under optimal experimental conditions, the response of the DPV was linearly proportional to the ETC concentration between 1.0×10−7 and 5.0×10−6 M ETC with a limit of detection (LOD) of 5.0×10−8 M. The developed sensor had excellent selectivity without detectable cross‐reactivity for levodopa and carbidopa. The MIP sensor was successfully used to detect ETC in spiked human serum samples.

Applications of Molecularly Imprinted Films

“…Electropolymerization was performed using CV by applying a potential between 0 and 0.8 V at a scan rate of 100 mV/s and electrochemical behavior of the films was studied using and DPV. As a result of the study, the sensor presented a good selectivity for entacapone levodopa and carbidopa, with a limit detection near 5 × 10 −8 M [93].…”

“…Radi and co-workers [93] have also described a sensor developed using MIP films that can be used for entacapone detection. A three-electrode system involving a WE (GCE), CE (platinum wire electrode) and RE (Ag/AgCl/KCl) was employed herein, while the layers were prepared using polyphenylenediamine (monomer) and entacapone (template).…”

A review on thin films, conducting polymers as sensor devices

“…Some typical examples of such sensors are briefed below. [108][109][110][111] etc. They are commonly for detecting toxic gasses and for measuring gas concentration.…”

Self-Reporting Molecularly Imprinted Polymer-Based Electrochemical Sensors for Structurally Similar Analytes

“…Molecularly imprinted polymers (MIPs) are the potential replacement for the natural receptors because of its good stability, molecular recognition property and low cost. MIPs are synthesized by polymerizing the functional monomer in presence of template molecule and subsequently creating the molecular cavity by removing the template from the three-dimensional polymer structure (1). MIPs are also known as artificial antibodies or plastibodies due to their recognition properties similar to natural antibodies.…”