Molecularly imprinted polymer based electrochemical biosensors: Overcoming the challenges of detecting vital biomarkers and speeding up diagnosis

Crapnell, Robert D.; Dempsey-Hibbert, Nina; Peeters, Marloes; Tridente, Ascanio; Banks, Craig E.

doi:10.1016/j.talo.2020.100018

Cited by 123 publications

(81 citation statements)

References 116 publications

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“…However, these are commonly based on enzyme linked immunosorbent assay (ELISA) methods that need expensive immune-chemicals, long response times and experienced people [68][69][70]. In this manner, molecular imprinted polymer technology can be a good alternative for the development of electrochemical affinity sensors for detection of biomarkers with high selectivity [71]. This technique is a new trend for creating "biomimetic receptors".…”

Section: Resultsmentioning

confidence: 99%

A New Nanomaterial Based Biosensor for MUC1 Biomarker Detection in Early Diagnosis, Tumor Progression and Treatment of Cancer

Ulucan‐Karnak

Akgöl

2021

Nanomanufacturing

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Early detection of cancer disease is vital to the successful treatment, follow-up and survival of patients, therefore sensitive and specific methods are still required. Mucin 1 (MUC1) is a clinically approved biomarker for determining the cancer that is a type I transmembrane protein with a dense glycosylated extracellular domain extending from the cell surface to 200–500 nm. In this study, nanopolymers were designed with a lectin affinity-based recognition system for MUC1 detection as a bioactive layer on electrochemical biosensor electrode surfaces. They were synthesized using a mini emulsion polymerization method and derivatized with triethoxy-3-(2-imidazolin-1-yl) propylsilane (IMEO) and functionalized with Concanavalin a Type IV (Con A) lectin. Advanced characterization studies of nanopolymers were performed. The operating conditions of the sensor system have been optimized. Biosensor validation studies were performed. Real sample blood serum was analyzed and this new method compared with a commercially available medical diagnostic kit (Enzyme-Linked ImmunoSorbent Assay-ELISA). The new generation nanopolymeric material has been shown to be an affordable, sensitive, reliable and rapid device with 0.1–100 U/mL linear range and 20 min response time.

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Section: Resultsmentioning

confidence: 99%

A New Nanomaterial Based Biosensor for MUC1 Biomarker Detection in Early Diagnosis, Tumor Progression and Treatment of Cancer

Ulucan‐Karnak

Akgöl

2021

Nanomanufacturing

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“…Although promising, there is little data to show interference studies and how changes in physiological matrixes would affect the system. In addition to these methods, there has been a recent surge in the use of molecularly imprinted polymers (MIPs) for sensor platforms [ 117 ]. These are polymer structures containing voids that match the size, shape, and functionalities of the target analyte.…”

Section: Current Non-invasive Technology For Lactate Measurementsmentioning

confidence: 99%

Evaluating the Possibility of Translating Technological Advances in Non-Invasive Continuous Lactate Monitoring into Critical Care

Crapnell

Tridente

Banks

et al. 2021

Sensors

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Lactate is widely measured in critically ill patients as a robust indicator of patient deterioration and response to treatment. Plasma concentrations represent a balance between lactate production and clearance. Analysis has typically been performed with the aim of detecting tissue hypoxia. However, there is a diverse range of processes unrelated to increased anaerobic metabolism that result in the accumulation of lactate, complicating clinical interpretation. Further, lactate levels can change rapidly over short spaces of time, and even subtle changes can reflect a profound change in the patient’s condition. Hence, there is a significant need for frequent lactate monitoring in critical care. Lactate monitoring is commonplace in sports performance monitoring, given the elevation of lactate during anaerobic exercise. The desire to continuously monitor lactate in athletes has led to the development of various technological approaches for non-invasive, continuous lactate measurements. This review aims firstly to reflect on the potential benefits of non-invasive continuous monitoring technology within the critical care setting. Secondly, we review the current devices used to measure lactate non-invasively outside of this setting and consider the challenges that must be overcome to allow for the translation of this technology into intensive care medicine. This review will be of interest to those developing continuous monitoring sensors, opening up a new field of research.

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“…The fabrication of MIPs using sol-gel techniques have been shown to improve performances of MIP sensors due to their inherent permeability, porosity, large surface area, and good physical rigidity [ 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Although a number of literature reports have been published on the development of MIP-based electrochemical sensor platforms for the detection of clinically relevant chemicals and biomarkers in spiked pre-treated biofluids or semi-synthetic plasma, there remain a number of challenges and drawbacks that ought to be overcome to produce sensor platforms with the needed specificity and sensitivity for clinical use [ 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

A Molecularly Imprinted Sol-Gel Electrochemical Sensor for Naloxone Determination

2021

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A molecularly imprinted sol-gel is reported for selective and sensitive electrochemical determination of the drug naloxone (NLX). The sensor was developed by combining molecular imprinting and sol-gel techniques and electrochemically grafting the sol solution onto a functionalized multiwall carbon nanotube modified indium-tin oxide (ITO) electrode. The sol-gel layer was obtained from acid catalyzed hydrolysis and condensation of a solution composed of triethoxyphenylsilane (TEPS) and tetraethoxysilane (TES). The fabrication, structure and properties of the sensing material were characterized via scanning electron microscopy, spectroscopy and electrochemical techniques. Parameters affecting the sensor’s performance were evaluated and optimized. A sensor fabricated under the optimized conditions responded linearly between 0.0 µM and 12 µM NLX, with a detection limit of 0.02 µM. The sensor also showed good run-to-run repeatability and batch-to-batch performance reproducibility with relative standard deviations (RSD) of 2.5–7.8% (n = 3) and 9.2% (n = 4), respectively. The developed sensor displayed excellent selectivity towards NLX compared to structurally similar compounds (codeine, fentanyl, naltrexone and noroxymorphone), and was successfully used to measure NLX in synthetic urine samples yielding recoveries greater than 88%.

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Molecularly imprinted polymer based electrochemical biosensors: Overcoming the challenges of detecting vital biomarkers and speeding up diagnosis

Cited by 123 publications

References 116 publications

A New Nanomaterial Based Biosensor for MUC1 Biomarker Detection in Early Diagnosis, Tumor Progression and Treatment of Cancer

A New Nanomaterial Based Biosensor for MUC1 Biomarker Detection in Early Diagnosis, Tumor Progression and Treatment of Cancer

Evaluating the Possibility of Translating Technological Advances in Non-Invasive Continuous Lactate Monitoring into Critical Care

A Molecularly Imprinted Sol-Gel Electrochemical Sensor for Naloxone Determination

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