Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

Panagiotopoulou, Maria; Salinas, Yolanda; Beyazıt, Selim; Kunath, Stephanie; Duma, Luminita; Prost, Élise; Mayes, Andrew G.; Resmini, Marina; Bui, Bernadette Tse Sum; Haupt, Karsten

doi:10.1002/ange.201601122

Cited by 50 publications

(27 citation statements)

References 42 publications

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“…Because QDs have the characteristics of fluorescence tunability, wide excitation wavelength and narrow emission wavelength, they can be used as multiplex probes to detect a variety of molecules by simultaneous multi‐color imaging at the same wavelength. Panagiotopoulou et al (2016) synthesized two kinds of molecularly imprinted polymer coated QDs that emit green and red fluorescence respectively, which can specifically recognize glucuronic acid and N‐acetylneuraminic acid respectively in human keratinocytes, and realize simultaneously multicolor imaging to these cells (Panagiotopoulou et al, 2016). Therefore, the combination of fluorescent QDs with this receptor ligand mechanism is of great significance in the specific cell imaging and diagnosis of tumors.…”

Section: The Application Of Qds To Cell Imagingmentioning

confidence: 99%

Research advance on cell imaging and cytotoxicity of different types of quantum Dots

Huang

Tang

2020

J of Applied Toxicology

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Quantum dots (QDs), as one of the emerging nanomaterials, have been widely studied by scientists due to their advantages and potential in bioimaging, especially cell imaging. Cadmium (Cd)‐based QDs, which have the best photoluminescence property, have received widespread attention. However, due to the obvious toxicity problem of these QDs, their cell imaging application is hindered. Recently, the emergence of Cd‐free‐metal and metal‐free QDs with lower toxicity makes people consider that Cd‐based QDs may be substituted by these QDs. However, problems of these QDs in cytotoxicity also cannot be ignored. Some reports claimed that their prepared emerging QDs for cell imaging were low toxicity, but there still exist up‐regulation of oxidative stress, inflammation, and apoptosis from other reports. And, up to now, few reports have separately summarized and discussed the issues of cell imaging and cytotoxicity of different types of QDs. Therefore, in this review, we classify QDs as following three types, Cd‐based, Cd‐free‐metal and metal‐free QDs, focus on the cell, the essential unit of life, discuss cell imaging application and cytotoxicity of QDs, and finally elucidate main mechanisms of cytotoxicity respectively. This review provides reference for the toxicity evaluation of QDs and highlights the potential cytotoxicity of Cd‐free QDs.

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Section: The Application Of Qds To Cell Imagingmentioning

confidence: 99%

Research advance on cell imaging and cytotoxicity of different types of quantum Dots

Huang

Tang

2020

J of Applied Toxicology

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“…In case of combination with photoelectric materials, MIPs could be used for bioimaging. Cancer targeting for MIPs was usually conducted according to the recognition of specific glycans on the tumor cell surface, such as glucuronic acid, sialic acid (SA), fucose (Fuc), mannose (Man), and so on [134][135][136][137][138]. Liu and co-workers reported SA-imprinted nanoparticles based on p-aminothiophenol (PATP, a Raman reporter)functionalized silver nanoparticles (AgNPs) for surface enhanced Raman scattering (SERS) imaging of cancer cells [134] (Fig.…”

Section: Bioimagingmentioning

confidence: 99%

Emerging functional materials based on chemically designed molecular recognition

Chen

Tian

et al. 2020

BMC Mat

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The specific interactions responsible for molecular recognition play a crucial role in the fundamental functions of biological systems. Mimicking these interactions remains one of the overriding challenges for advances in both fundamental research in biochemistry and applications in material science. However, current molecular recognition systems based on host-guest supramolecular chemistry rely on familiar platforms (e.g., cyclodextrins, crown ethers, cucurbiturils, calixarenes, etc.) for orienting functionality. These platforms limit the opportunity for diversification of function, especially considering the vast demands in modern material science. Rational design of novel receptor-like systems for both biological and chemical recognition is important for the development of diverse functional materials. In this review, we focus on recent progress in chemically designed molecular recognition and their applications in material science. After a brief introduction to representative strategies, we describe selected advances in these emerging fields. The developed functional materials with dynamic properties including molecular assembly, enzymelike and bio-recognition abilities are highlighted. We have also selected materials with dynamic properties in contract to traditional supramolecular host-guest systems. Finally, the current limitations and some future trends of these systems are discussed.

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“…Nonetheless, many biosensors and POC platforms have been developed with multiplexing capabilities, although the same level of progression has not been observed for biomimetic sensing, with few reports of multiplexed sensing strategies and none for the detection of cardiac biomarkers. However, multi-marker analysis has been implemented for some biomimetic sensors, with several groups utilising QDs that were excited by a common wavelength and subsequently emitted distinct wavelengths without any spectral overlap and thus enabling the measurement of individual analytes [156,157,158]. Biomimetic multi-marker detection is not only confined to luminescence-based detection mechanisms, as one group have produced a QCM sensor array for the detection of three analytes [159].…”

Section: Detection Techniquesmentioning

confidence: 99%

Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

Regan

Walsh

O’Kennedy

et al. 2019

Sensors

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Molecular imprinting is a rapidly growing area of interest involving the synthesis of artificial recognition elements that enable the separation of analyte from a sample matrix and its determination. Traditionally, this approach can be successfully applied to small analyte (<1.5 kDa) separation/ extraction, but, more recently it is finding utility in biomimetic sensors. These sensors consist of a recognition element and a transducer similar to their biosensor counterparts, however, the fundamental distinction is that biomimetic sensors employ an artificial recognition element. Molecularly imprinted polymers (MIPs) employed as the recognition elements in biomimetic sensors contain binding sites complementary in shape and functionality to their target analyte. Despite the growing interest in molecularly imprinting techniques, the commercial adoption of this technology is yet to be widely realised for blood sample analysis. This review aims to assess the applicability of this technology for the point-of-care testing (POCT) of cardiovascular disease-related biomarkers. More specifically, molecular imprinting is critically evaluated with respect to the detection of cardiac biomarkers indicative of acute coronary syndrome (ACS), such as the cardiac troponins (cTns). The challenges associated with the synthesis of MIPs for protein detection are outlined, in addition to enhancement techniques that ultimately improve the analytical performance of biomimetic sensors. The mechanism of detection employed to convert the analyte concentration into a measurable signal in biomimetic sensors will be discussed. Furthermore, the analytical performance of these sensors will be compared with biosensors and their potential implementation within clinical settings will be considered. In addition, the most suitable application of these sensors for cardiovascular assessment will be presented.

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Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

Cited by 50 publications

References 42 publications

Research advance on cell imaging and cytotoxicity of different types of quantum Dots

Research advance on cell imaging and cytotoxicity of different types of quantum Dots

Emerging functional materials based on chemically designed molecular recognition

Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

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