Molecularly imprinted polymer for analysis of zidovudine and stavudine in human serum by liquid chromatography–mass spectrometry

Duy, Sung Vo; Lefebvre-Tournier, Isabelle; Pichon, Valérie; Hugon-Chapuis, F.; Puy, Jean-Yves; Philouze, Christian

doi:10.1016/j.jchromb.2009.02.068

Cited by 26 publications

(13 citation statements)

References 23 publications

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“…The literature is lacking in studies involving the use of MIP for extraction of ARVs from human plasma. A typical comparison with other methods reported in the literature using SPE for the determination of 3TC, AZT and EFZ in different matrices is presented in Supporting Information Table 3 , which confirm that LOQ value is appropriate and comparable with other research works. The low LOQs are mainly attributed to the use of highly sensitive, complex and expensive detector, i.e.…”

Section: Resultssupporting

confidence: 83%

Hollow mesoporous structured molecularly imprinted polymer as adsorbent in pipette‐tip solid‐phase extraction for the determination of antiretrovirals from plasma of HIV‐infected patients

et al. 2018

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In this work a hollow mesoporous structured molecularly imprinted polymer was synthetized and used as adsorbent in pipette-tip solid-phase extraction for the determination of lamivudine (3TC), zidovudine (AZT) and efavirenz (EFZ) from plasma of human immunodeficiency virus (HIV) infected patients by high-performance liquid chromatography (HPLC). All parameters that influence the recovery of the pipette tip based on hollow mesoporous molecularly imprinted polymer solid-phase extraction (PT-HM-MIP-SPE) method were systematically studied and discussed in detail. The adsorbent material was prepared using methacrylic acid and 4-vinylpyridine as functional monomers, ethylene glycol dimethacrylate as crosslinker, acetonitrile as solvent, 4,4'-azobis(4-cyanovaleric acid) as radical initiator, benzalkonium chloride as surfactant, 3TC, and AZT as templates. The simultaneous separation of 3TC, AZT and EFZ by HPLC-UV was performed using a Gemini C18 Phenomenex® column (250 mm × 4.6 mm, 5 μm) and mobile phase consisting of acetonitrile: water pH 3.2 (68:32, v/v), flow rate of 1.0 mL/min and λ = 260 nm. The method was linear over the concentration range from 0.25 to 10 μg/mL for 3TC and EFZ, and 0.05 to 2.0 μg mL for AZT, with correlation coefficients larger than 0.99 for all analytes. Recovery ± relative standard deviations (RSDs %) were 41.99 ± 2.38%, 82.29 ± 1.63%, and 83.72 ± 7.52% for 3TC, AZT, and EFZ, respectively. The RSDs and relative errors (REs) were lower than 15% for intra and interday assays. The method has been successfully applied for monitoring HIV-infected patients outside the therapeutic dosage.

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Section: Resultssupporting

confidence: 83%

Hollow mesoporous structured molecularly imprinted polymer as adsorbent in pipette‐tip solid‐phase extraction for the determination of antiretrovirals from plasma of HIV‐infected patients

et al. 2018

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“…Concerning the number of binding sites (N), the value for MIP 6 throughout the entire affinity range (N = 10.22 lmol g À1 or 1.39 mg g À1 ) lies within the range typically found for non-covalent MIPs (0.1-3.5 mg g À1 ) [55,56]. Furthermore, within the measured concentration window, the number of binding sites for MIPs 3 and 6 are of the same order of magnitude than their control polymers whereas MIPs 1, 2, 4, and 5 have considerable lower capacities (N) compared to the corresponding NIPs.…”

Section: Binding Isothermssupporting

confidence: 55%

Selective removal of ATP degradation products from food matrices I: Design and characterization of a dummy molecularly imprinted specific sorbent for hypoxanthine

Latorre

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Fernández

et al. 2015

Reactive and Functional Polymers

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“…Non-imprinted polymers (NIP 1 to NIP 5), were synthesized following the same procedure as their corresponding MIP except that the template was absent. The five polymers were generated using a ratio 1/4/20,the most common ratio used in the literature [25][26][27][28][29][30][31], between the template, the monomer, and the cross linker. For all the syntheses, the template (1 mmol) and monomer (4 mmol) were first mixed with 1.8 mL of the corresponding porogen in a glass vial the "template-monomer" complex was allowed to form in an ultra-sonication bath for 10 minutes.…”

Section: Synthesis Of Molecularly Imprinted Polymers and Preparation mentioning

confidence: 99%

Development and application of water-compatible molecularly imprinted polymers for the selective extraction of carbamazepine from environmental waters

et al. 2019

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A molecularly imprinted polymer (MIP) was designed in order to allow the selective solid-phase extraction of carbamazepine (CBZ), an anticonvulsant and mood-stabilizing drug, at ultra-trace level from aqueous environmental samples. A structural analog of CBZ was selected as a dummy template and different synthesis conditions were screened. The selectivity of the resulting imprinted polymers was evaluated by studying the retention of CBZ in a solvent similar to the one used for the synthesis. The presence of imprinted cavities in the polymers was then demonstrated by comparing the elution profiles (obtained by using MIP and a non-imprinted polymer, NIP, as a control) of the template, of CBZ, and of a structural analog of CBZ. Then the extraction procedure was further optimized for the treatment of aqueous samples on the two most promising MIPs, with a special attention being paid to the volume and composition of the percolation and washing solutions. The best MIP provided a highly selective retention in tap water with 81 % extraction recovery for CBZ in the elution fraction of the MIP and only 14% for NIP. The repeatability of the extraction procedure was demonstrated for both tap and river waters (RSD below 4% in river water) for the drugs CBZ, oxcarbamazepine, and one metabolite (carbamazepine 10,11-epoxide). A MIP capacity of 1.15 µmol g-1 was determined. Finally, an analytical procedure involving the MIP was developed allowing the detection of CBZ at a concentration level of only a few ng L-1 in river water. The selectivity provided by the MIP resulted in a 3000-fold increase of the signal-to-noise ratio in LC/MS analysis as compared to the use of conventional sorbent.

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Molecularly imprinted polymer for analysis of zidovudine and stavudine in human serum by liquid chromatography–mass spectrometry

Cited by 26 publications

References 23 publications

Hollow mesoporous structured molecularly imprinted polymer as adsorbent in pipette‐tip solid‐phase extraction for the determination of antiretrovirals from plasma of HIV‐infected patients

Hollow mesoporous structured molecularly imprinted polymer as adsorbent in pipette‐tip solid‐phase extraction for the determination of antiretrovirals from plasma of HIV‐infected patients

Selective removal of ATP degradation products from food matrices I: Design and characterization of a dummy molecularly imprinted specific sorbent for hypoxanthine

Development and application of water-compatible molecularly imprinted polymers for the selective extraction of carbamazepine from environmental waters

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