Molecularly imprinted polymers as biomimetic catalysts

Resmini, Marina

doi:10.1007/s00216-011-5671-2

Cited by 96 publications

(57 citation statements)

References 33 publications

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“…Redox enzymes have been mimicked by integrating metal ions or metal complexes into the polymer matrix of MIPs [25][26][27][28][29]. Moreover, catalytic MIPs have been obtained for reactions for which no natural enzyme catalyst exists [30].…”

Section: Preparation Of Surface Imprinted Mipsmentioning

confidence: 99%

Enzymes as Tools in MIP-Sensors

et al. 2017

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Abstract:Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as "tracers" for the generation and amplification of the measuring signal. In addition, enzymatic pretreatment of an analyte can extend the analyte spectrum and eliminate interferences.

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Section: Preparation Of Surface Imprinted Mipsmentioning

confidence: 99%

Enzymes as Tools in MIP-Sensors

et al. 2017

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“…2). Wulff's group [59][60][61] and Resmini's group [62,63] have pioneered in this area. Several polymers mimicking the hydrolytic activity of some enzymes have been synthesized using the TSA approximation, which stabilized the intermediate state and lowered the activation energy.…”

Section: Mip-based Quartz Crystal Microbalance Bioassaysmentioning

confidence: 99%

Molecularly Imprinted Polymers as Tools for Bioassays and Biotransformation

Liu

Huang

et al. 2015

Molecularly Imprinted Polymers in Biotechnology

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In the past five years, significant progress has been made in preparation of various molecularly imprinted polymer (MIP)-based materials for applications in bioassays and biotransformation. This chapter reviews the important advances in these two fields. The first part mainly focuses on the development of various MIP-based bioassays that convert the rebinding of template to the imprinted cavities into measurable luminescent signals, including fluorescence, phosphorescence, Raman scattering, diffraction, and the like. In addition, MIP-based bioassays that are measured by surface plasmon resonance or quartz crystal microbalance are also discussed. In the following part, representative biotransformation reactions that make use of MIPs are summarized. In the last part of this chapter, some remaining challenges are briefly discussed for further development of the two fields.

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“…MIPs have many advantages, including high selectivity, mechanical and chemical stability, low cost and easy preparation, and a long storage life. Over the past decades, these polymers have been successfully used in different fields [10][11][12][13][14][15] such as chemical sensors [16][17][18][19][20], enzyme mimicking catalysis [21][22][23], intelligent drug delivery [24][25][26][27], etc. In particular, MIPs have been extensively employed as the adsorbents of the solid phase extraction (SPE) for the selective extraction of a target compound and structural analogues from the complex matrices, such as natural products [28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymers for Selective Extraction of Oblongifolin C from <em>Garcinia yunnanensis</em> Hu

Wang¹,

Wenwei²,

Shen³

et al. 2017

Preprint

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Molecularly imprinted polymers (MIPs) were synthesized and applied for the selective extraction of oblongifolin C (OC) from fruit extracts of Garcinia yunnanensis Hu. A series of experiments and computational approaches were employed to improve the efficiency of screening for optimal MIP systems in the study. The molar ratio (1:4) was chosen at last based on the comparison of the binding energy of the complexes between the template (OC) and functional monomers using density functional theory (DFT) at the RI-PBE-D3-gCP/def2-TZVP level of theory. The binding characterization and the molecular recognition mechanism of MIPs were further explained using the molecular modeling method and NMR and IR spectra data. The reusability of this approach was demonstrated in over 20 batch rebinding experiments. 140.5 mg of OC (>95% purity) was obtained from the 5 g extracts with 2 g of MIPs with the best binding properties through gradient elution program from 35% to 70% methanol-water solution. At the same time, another structural analog, 46.5 mg of guttiferone K (GK) (>88% purity), was also obtained by the gradient elution procedure. Our results showed that the structural analogs could be separated from the crude extracts by the molecularly imprinted solid-phase extraction (MISPE) using a gradient elution procedure for the first time.

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Molecularly imprinted polymers as biomimetic catalysts

Cited by 96 publications

References 33 publications

Enzymes as Tools in MIP-Sensors

Enzymes as Tools in MIP-Sensors

Molecularly Imprinted Polymers as Tools for Bioassays and Biotransformation

Molecularly Imprinted Polymers for Selective Extraction of Oblongifolin C from <em>Garcinia yunnanensis</em> Hu

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