Molecularly imprinted polymers as effective capturing receptors in a pseudo-ELISA immunoassay for procalcitonin detection in veterinary species

Battaglia, Federica; Bonelli, Francesca; Sgorbini, Micaela; Intorre, Luigi; Minunni, Maria; Scarano, Simona; Meucci, Valentina

doi:10.1039/d2ay01175a

Cited by 10 publications

(1 citation statement)

References 79 publications

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“…Similarly, MIPs can be imprinted with bone loss biomarkers incorporated with quantum dots to help monitor bone activity. Battaglia et al demonstrated that MIPs can be used in pseudo-ELISA immune assays by replacing the capture antigen with imprinted procalcitonin on a (polynorepinephrine)-based polymer and proved that it can be a viable technique . Various ways in which MIPs can be incorporated into sensing are represented in Figure .…”

Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 99%

Molecularly Imprinted Polymers: Shaping the Future of Early-Stage Bone Loss Detection─A Review

Agnishwaran,

Manivasagam,

Udduttula

2024

ACS Omega

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Osteoporosis is the deterioration of bone mineral density (BMD) because of an imbalance between bone resorption and formation, which might happen due to lots of factors like age, hormonal imbalance, and several others. While this occurrence is prevalent in both genders, it is more common in women, especially postmenopausal women. It is an asymptomatic disease that is underlying until the first incidence of a fracture. The bone is weakened, making it more susceptible to fracture. Even a low trauma can result in a fracture, making osteoporosis an even more alarming disease. These fractures can sometimes be fatal or can make the patient bedridden.Osteoporosis is an understudied disease, and there are certain limitations in diagnosing and early-stage detection of this condition. The standard method of dual X-ray absorptiometry can be used to some extent and can be detected in standard radiographs after the deterioration of a significant amount of bone mass. Clinically assessing osteoporosis using biomarkers can still be challenging, as clinical tests can be expensive and cannot be accessed by most of the general population. In addition, manufacturing antibodies specific to these biomarkers can be a challenging, time-consuming, and expensive method. As an alternative to these antibodies, molecularly imprinted polymers (MIPs) can be used in the detection of these biomarkers. This Review provides a comprehensive exploration of bone formation, resorption, and remodeling processes, linking them to the pathophysiology of osteoporosis. It details biomarker-based detection and diagnosis methods, with a focus on MIPs for sensing CTX-1, NTX-1, and other biomarkers. The discussion compares traditional clinical practices with MIP-based sensors, revealing comparable sensitivity with identified limitations. Additionally, the Review contrasts antibody-functionalized sensors with MIPs. Finally, our Review concludes by highlighting the potential of MIPs in future early-stage osteoporosis detection.

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Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 99%

Molecularly Imprinted Polymers: Shaping the Future of Early-Stage Bone Loss Detection─A Review

Agnishwaran,

Manivasagam,

Udduttula

2024

ACS Omega

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Direct competitive immunoassay method for sensitive detection of the histamine in foods based on a MI‐Cu‐GMP nanozyme marker and molecularly imprinted biomimetic antibody

Peng,

Wang,

et al. 2024

J Sci Food Agric

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BACKGROUNDHistamine may lead to low blood pressure, skin flushing and edema when it accumulates in large amounts in the body. Therefore, establishing sensitive methods for the detection of histamine in foods is extremely important to ensure food safety and human health.RESULTSThe MI‐Cu‐GMP NPs (2‐methylimidazole‐copper‐guanosine monophosphate nanozymes) with high laccase‐like activity were synthesized. Using the prepared molecular imprinted membrane as biomimetic antibody and MI‐Cu‐GMP NPs as marker, a sensitive direct competitive biomimetic enzyme‐linked immunoassay (BELISA) method for rapid detection of the histamine in foods was developed. Under optimal conditions, the limit of detection (LOD, IC15) and sensitivity (IC50) of the BELISA method for histamine was 0.05 mg L−1 and 1.22 mg L−1, respectively. The liquor samples spiked with histamine was detected by the BELISA method with satisfactory recoveries ranging from 90.00% to 116.00%. Further, the level of histamine in three samples (cooking wine, rice vinegar and soy sauce) was tested by the BELISA and high‐performance liquid chromatography (HPLC), with no significant difference found between the two methods.CONCLUSIONGiven the advantages, the established BELISA method is expected to provide practical guidance for the monitoring of histamine in food and provides a foundation for the detection of other food hazards. © 2024 Society of Chemical Industry.

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Fundamentals of chemical sensors and biosensors

Yang,

2024

Machine Learning and Artificial Intelligence in Chemical and Biological Sensing

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Molecularly imprinted polymers as effective capturing receptors in a pseudo-ELISA immunoassay for procalcitonin detection in veterinary species

Abstract: In this study, a new sandwich-type immunoenzymatic assay, based on a molecularly imprinted polymer (MIP) as an artificial antibody (pseudo-ELISA), was developed for the determination of procalcitonin (PCT) in veterinary...

Cited by 10 publications

References 79 publications

Molecularly Imprinted Polymers: Shaping the Future of Early-Stage Bone Loss Detection─A Review

Molecularly Imprinted Polymers: Shaping the Future of Early-Stage Bone Loss Detection─A Review

Direct competitive immunoassay method for sensitive detection of the histamine in foods based on a MI‐Cu‐GMP nanozyme marker and molecularly imprinted biomimetic antibody

Fundamentals of chemical sensors and biosensors

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