Molecularly imprinted polymers for separating and sensing of macromolecular compounds and microorganisms

Iskierko, Zofia; Sharma, Piyush Sindhu; Bartold, Katarzyna; Pietrzyk‐Le, Agnieszka; Noworyta, Krzysztof; Kutner, Włodzimierz

doi:10.1016/j.biotechadv.2015.12.002

Cited by 112 publications

(63 citation statements)

References 173 publications

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“…Surface grafting imprinting is another effective approach to overcome the difficulty of template mass transfer within MIPs and particularly valuable when considering the imprinting of macromolecular structures such as proteins, polysaccharides or microorganisms [91]. …”

Section: Imprinting Technologymentioning

confidence: 99%

Imprinting Technology in Electrochemical Biomimetic Sensors

Frasco

Truta

Sales

et al. 2017

Sensors

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Biosensors are a promising tool offering the possibility of low cost and fast analytical screening in point-of-care diagnostics and for on-site detection in the field. Most biosensors in routine use ensure their selectivity/specificity by including natural receptors as biorecognition element. These materials are however too expensive and hard to obtain for every biochemical molecule of interest in environmental and clinical practice. Molecularly imprinted polymers have emerged through time as an alternative to natural antibodies in biosensors. In theory, these materials are stable and robust, presenting much higher capacity to resist to harsher conditions of pH, temperature, pressure or organic solvents. In addition, these synthetic materials are much cheaper than their natural counterparts while offering equivalent affinity and sensitivity in the molecular recognition of the target analyte. Imprinting technology and biosensors have met quite recently, relying mostly on electrochemical detection and enabling a direct reading of different analytes, while promoting significant advances in various fields of use. Thus, this review encompasses such developments and describes a general overview for building promising biomimetic materials as biorecognition elements in electrochemical sensors. It includes different molecular imprinting strategies such as the choice of polymer material, imprinting methodology and assembly on the transduction platform. Their interface with the most recent nanostructured supports acting as standard conductive materials within electrochemical biomimetic sensors is pointed out.

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Section: Imprinting Technologymentioning

confidence: 99%

Imprinting Technology in Electrochemical Biomimetic Sensors

Frasco

Truta

Sales

et al. 2017

Sensors

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“…Further challenges to be resolved include the regeneration of macromolecularly imprinted polymers. 26 …”

Section: Discussionmentioning

confidence: 99%

Long-term stability and reusability of molecularly imprinted polymers

et al. 2017

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“…Receptors, aptamers, enzymes, nucleic acids, antibodies and lectins are placed among biological recognition elements in this technology [12,13]. In many of the studies dealing with microbial detection, antibodies were used in order to recognize and separate target cells.…”

Section: Use Of Molecularly Imprinted Polymers In Affinity Recognimentioning

confidence: 99%

“…However, natural receptors tend to denature in conditions outside the physiological range such as elevated/lowered temperature, strongly acidic/basic conditions, presence of organic-rich solvents etc. [3,12]. One additional limitation of biological recognition structures is the fact that they are easily degradable by e.g., proteases.…”

Section: Use Of Molecularly Imprinted Polymers In Affinity Recognimentioning

confidence: 99%

Imprinting of Microorganisms for Biosensor Applications

İdil

Mattìasson

2017

Sensors

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There is a growing need for selective recognition of microorganisms in complex samples due to the rapidly emerging importance of detecting them in various matrices. Most of the conventional methods used to identify microorganisms are time-consuming, laborious and expensive. In recent years, many efforts have been put forth to develop alternative methods for the detection of microorganisms. These methods include use of various components such as silica nanoparticles, microfluidics, liquid crystals, carbon nanotubes which could be integrated with sensor technology in order to detect microorganisms. In many of these publications antibodies were used as recognition elements by means of specific interactions between the target cell and the binding site of the antibody for the purpose of cell recognition and detection. Even though natural antibodies have high selectivity and sensitivity, they have limited stability and tend to denature in conditions outside the physiological range. Among different approaches, biomimetic materials having superior properties have been used in creating artificial systems. Molecular imprinting is a well suited technique serving the purpose to develop highly selective sensing devices. Molecularly imprinted polymers defined as artificial recognition elements are of growing interest for applications in several sectors of life science involving the investigations on detecting molecules of specific interest. These polymers have attractive properties such as high bio-recognition capability, mechanical and chemical stability, easy preparation and low cost which make them superior over natural recognition reagents. This review summarizes the recent advances in the detection and quantification of microorganisms by emphasizing the molecular imprinting technology and its applications in the development of sensor strategies.

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Molecularly imprinted polymers for separating and sensing of macromolecular compounds and microorganisms

Cited by 112 publications

References 173 publications

Imprinting Technology in Electrochemical Biomimetic Sensors

Imprinting Technology in Electrochemical Biomimetic Sensors

Long-term stability and reusability of molecularly imprinted polymers

Imprinting of Microorganisms for Biosensor Applications

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