Molecularly imprinted polymers for the selective extraction of tiliroside from the flowers of Edgeworthia gardneri (wall.) Meisn

Abstract

Nano-sized molecularly imprinted polymers for tiliroside were successfully prepared by a precipitation polymerization method. Acrylamide, ethylene glycol dimethacrylate, azobisisobutyronitrile, and acetonitrile/dimethyl sulfoxide were used as functional monomer, cross-linker, initiator, and porogen, respectively. The structural features and morphological characterization of tiliroside-imprinted polymers were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy, respectively. The adsorption experiments indicated that the tiliroside-imprinted polymers exhibited high selective recognition property to tiliroside. Scatchard analysis indicated that the homogeneous-binding sites were formed in the polymers. The selectivity test revealed that the adsorption capacity and selectivity of polymers to tiliroside was significantly higher than that of rutin, astragalin, and kaempferol. Finally, the tiliroside-imprinted polymers were employed as adsorbents in solid-phase extraction for the extraction of tiliroside from the ethyl acetate extract of the flowers of Edgeworthia gardneri (wall.) Meisn. The results demonstrated that the extraction recoveries of tiliroside ranged from 69.3 to 73.5% by using tiliroside-imprinted polymers coupled with solid-phase extraction method. These results indicated that the tiliroside-based molecularly imprinted solid-phase extraction method was proven to be an effective technique for the separation and enrichment of tiliroside from natural medicines.

A Review on the Dietary Flavonoid Tiliroside

“…Unfortunately, only one paper reports the use of one of these procedures (Gao et al., ), which applied molecular imprinted polymers (MIPs) for the selective extraction of tiliroside from the flowers of Edgeworthia gardneri (Wall.) Meisn.…”

Rapidly colorimetric detection of caffeine in beverages by silver nanoparticle sensors coupled with magnetic molecularly imprinted polymeric microspheres

“…For higher selectivity, some new approaches were developed, such as antibody‐based and aptamer‐based methods to isolate or enrich chemicals from complicated food matrices, but the great challenges are high cost and laborious manufacture of antibody and aptamer for specific analytes. In comparison, molecularly imprinted polymers (MIPs) with high recognition towards target analyte have been used to effectively pretreat samples in the recent years (Gao et al ., , ). However, when extracting target substance by MIP, it is often necessary to prepare a solid‐phase extraction packed column to facilitate the separation of the MIP from the solution.…”

New phases for analytical scale extraction from plants: Current and future trends

“…antibiotics, pollutants and toxins) from food or environmental samples is one of the most highly developed applications of MIPs in analytical chemistry [77][78][79]. The use of MIPs in plant analyses follows a different trend, that is the extraction of endogenous bioactive compounds, including molecules with widely differing chemical structures, thanks to the possibility of obtaining tailor-made MIPs [80][81][82][83][84][85][86].…”