Molecularly Imprinted Sol−Gel Nanotubes Membrane for Biochemical Separations

Abstract

In this study, we report a simple procedure for applying molecular imprinting functional groups to the inner surfaces of the template-synthesized sol-gel nanotubes for chemical separation of estrone. The silica nanotubes were synthesized within the pores of nanopore alumina template membranes using a sol-gel method by simultaneous hydrolysis of a silica monomer-imprinted molecule complex and tetraethoxysilane (TEOS). A covalent imprinting strategy was employed by generating a sacrificial spacer through the reaction of the isocyanate group of 3-(triethoxysilyl)propyl isocyanate and a phenol moiety of estrone to form a thermally cleavable urethane bond. This allowed us to remove the imprinted estrone by simple thermal reaction and to simultaneously introduce functional groups into the cavity formed by the silica nanotubes. Experiments indicated that estrone could be bound selectively by such an approach and have a binding affinity of 864 +/- 137 (n = 3).

Composite of CdTe quantum dots and molecularly imprinted polymer as a sensing material for cytochrome c

“…So to design and develop artificial receptors for target molecular recognition is one way to answer this challenge (Hoshino et al, 2008;Schirhagl et al, 2010). While this approach has already been used successfully in molecular imprinting which is a technique for the synthesis of artificial receptors under the guidance of print molecule to fabricate complementary binding sites with specific recognition ability (Matsui et al, 2007;Kempe and Mosbach, 1995;Yang et al, 2004;Ouyang et al, 2007;Tong et al, 2001). Furthermore, in contrast to the natural antibody, the substantial advantages of artificial counterparts are their mechanical and chemical robustness and their production by self-assembling processes without time-consuming and complex synthesis.…”

Preparation of Molecularly Imprinted Composite Membranes for Inducing Bergenin Crystallization in Supercritical CO2and Adsorption Properties

“…11 In MIMs systems, the combination of the imprinting technique can provide membranes with specific selectivity for the separation of targeted organic compounds and thus make the MIMs possess the advantages of both molecular imprinting and membrane technology. [12][13][14] According to the preparation methods, MIMs can be generally divided into three categories, namedly molecularly imprinted filling membranes (MIFMs), molecularly imprinted monolithic membranes (MIMMs), and molecularly imprinted composite membranes (MICMs), 15 among which MICMs attract more attention because of their high flux of the resulted composite membranes and well flexibility of applications.…”

Plastic antibody for DNA damage: fluorescent imaging of BPDE–dG adducts in genomic DNA

“…[19][20][21] The chemistry-based molecular imprinting technique may be a better choice to fabricate articial antibodies, and it is currently a promising tool for the creation of antibody-mimicking polymers, namely molecularly imprinted polymers (MIPs), due to their remarkable specicity and chemical robustness. [22][23][24][25][26][27][28] MIPs for 8-OHdG, a biomarker for DNA oxidative damage, have been prepared and applied in highly-sensitive biosensing of nanomolar 8-OHdG in human blood and urine samples. 29,30 A challenge in exploring MIPs as articial antibodies is to attain higher affinity, which needs to be sufficient for specic binding of targets from an excess of complex matrix.…”

“…31,32 They may have many advantages over the traditional MIP particles, including homogenous binding site distribution, a high yield of high-affinity sites and good site accessibility. 24,[31][32][33] Atom transfer radical polymerization (ATRP), a novel living/ controlled radical polymerization technique, has been successfully used for the fabrication of nanoscaled MIPs with ordered structures and improved binding properties. 33,35,36 ATRP allows polymers to be grown in a controlled, piece-by-piece fashion, and enables researchers to create a wide range of polymers with site specic tailored functionalities for various applications.…”