Molecule-binding dependent assembly of split aptamer and γ-cyclodextrin: A sensitive excimer signaling approach for aptamer biosensors

Jin, Fen; Lian, Yan; Li, Jishan; Zheng, Jing; Hu, Yaping; Liu, Jinhua; Huang, Jin; Yang, Ronghua

doi:10.1016/j.aca.2013.08.012

Cited by 23 publications

(29 citation statements)

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“…The design of conformation-based aptamer probes can be divided into three categories, including intramolecular switch, intermolecular switch, and replacement of DNA strand [22][23][24] Fig. (2).…”

Section: Conversion Of Conformationmentioning

confidence: 99%

“…Schematic illustration of conversion of aptamer conformation. (a) Intramolecular switch [22], (b) Intermolecular switch [23], (c) Replacement of DNA strand [24]. In 2001, Hamaguchi et al reported using an aptamer molecular beacon probe to determine the concentration of thrombin.…”

Section: Fig (2)mentioning

confidence: 99%

“…In the presence of the target, two free aptamer sequences hybridize to quench the fluorescent signal [38]. This strategy is suitable for the design of aptamers for most targets, including Pb 2+ [19], ATP [23] and streptomycin [39], etc. Of course, it is generally necessary to optimize the design of sequences.…”

Section: Intermolecular Switchmentioning

confidence: 99%

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The Design and Improvement of Aptamer-based Fluorescent Probes

Zou

Zhao

et al. 2019

CSM

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Fluorescent probes exhibit the characteristics of high sensitivity, diversified design, and strong quantitative analysis ability. Therefore, they have become one of the research hotspots in analytical chemistry and biological analysis. Aptamers are single-stranded DNAs or RNAs that are obtained in vitro and can selectively bind to the targets, making them ideal candidates as identifying units in fluorescent probes. However, most of the current aptamer probes are not regulatable because their fluorescence signals can't be changed after the fluorescent probes are bound to the target, resulting in a high signal background and limited contrast. Actually, the structure of aptamers is flexible and can be combined with a variety of nanomaterials to achieve high sensitivity fluorescence for quantitative analysis, and can be used for a variety of target determinations, such as metal ions, small molecules, proteins and cells. In addition, with the development of research on DNA nanotechnology, researchers have designed many aptamer-based fluorescent probes with special properties, which have good application prospects in complex sample analyses and even in vivo cell imaging. In this review, the development of research in the field of aptamer-based fluorescent probes is surveyed and four mechanisms of aptamer fluorescent probes, including dye intercalation, conversion of DNA conformation, signal amplification, and mediation of nanomaterials, are comprehensively discussed.

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Section: Conversion Of Conformationmentioning

confidence: 99%

Section: Fig (2)mentioning

confidence: 99%

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The Design and Improvement of Aptamer-based Fluorescent Probes

Zou

Zhao

et al. 2019

CSM

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“…This inclusion interaction of γ-CD with stem labels offers unprecedented, new, easy and predictable degrees of freedom to tune stem stability, allowing for the facile introduction of further functionalities of DNA probes to increase signal-to-background ratio and improve target binding specificity for multiplexing. 57-58 …”

Section: Rationally Designed Molecular Beaconsmentioning

confidence: 99%

Rationally designed molecular beacons for bioanalytical and biomedical applications

et al. 2015

Self Cite

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Nucleic acids hold promise as biomolecules for future applications in biomedicine and biotechnology. Their well-defined structures and compositions afford unique chemical properties and biological functions. Moreover, the specificity of hydrogen-bonded Watson-Crick interactions allows the construction of nucleic acid sequences with multiple functions. In particular, the development of nucleic acid probes as essential molecular engineering tools will make a significant contribution to advancements in biosensing, bioimaging and therapy. The molecular beacon (MB), first conceptualized by Tyagi and Kramer in 1996, is an excellent example of a double-stranded nucleic acid (dsDNA) probe. Although inactive in the absence of target, dsDNA probes can report the presence of a specific target through hybridization or specific recognition-triggered change in conformation. MB probes are typically fluorescently labeled oligonucleotides that range from 25 to 35 nucleotides (nt) in length, and their structure can be divided into three components: stem, loop and reporter. The intrinsic merit of MBs depends on predictable design, reproducibility of synthesis, simplicity of modification, and built-in signal transduction. Using resonance energy transfer (RET) for signal transduction, MBs are further endowed with increased sensitivity, rapid response and universality, making them ideal for chemical sensing, environmental monitoring and biological imaging, in contrast to other nucleic acid probes. Furthermore, integrating MBs with targeting ligands or molecular drugs can substantially support their in vivo applications in theranositics. In this review, we survey advances in bioanalytical and biomedical applications of rationally designed MBs, as they have evolved through the collaborative efforts of many researchers. We first discuss improvements to the three components of MBs: stem, loop and reporter. The current applications of MBs in biosensing, bioimaging and therapy will then be described. In particular, we emphasize recent progress in constructing MB-based biosensors in homogeneous solution or on solid surfaces. We expect that such rationally designed and functionalized MBs will open up new and exciting avenues for biological and medical research and applications.

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“…Topuz et al reported that cyclodextrin functionalized mesostructured silica nanoparticles were used to remove polycyclic aromatic hydrocarbons [34]. To date, cyclodextrins are widely used in various fields such as drug delivery, molecular recognition, self-assembly, extraction of cholesterol from food, electrochemical applications, cosmetics, biosensors and adsorption of environmental pollutants [35][36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of β-cyclodextrin modified mesostructured silica coated multi-walled carbon nanotubes composites and application for paraben removal

Ding

et al. 2018

Water Science and Technology

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In this work, the novel β-cyclodextrin modified mesostructured silica coated multi-walled carbon nanotubes (MWCNTs) composites were synthesized and applied for the removal of parabens in aqueous solution. The prepared MWCNTs/SiO2/β-CD composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy and thermogravimetric analysis. The effects of the amount of adsorbent, pH and elution solvents on the removal efficiency of parabens from water solutions were investigated. Under the optimized conditions, over 95% removal efficiency was achieved by using 40 mg of MWCNTs/SiO2/β-CD adsorbents to absorb the parabens from 60 mL of 0.5 μg/mL parabens solutions. The solution pH in the range from 5 to 9 has no influence on the removal efficiency and the parabens sorption capacity of the prepared adsorbents were around 0.75 μg/mg. Furthermore, the stability and reusability studies demonstrated that the prepared MWCNTs/SiO2/β-CD composites are cost-effective adsorbents for the removal of parabens from water with high regeneration efficiency. The composites fabricated in this study could become an attractive candidate for water purification.

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Molecule-binding dependent assembly of split aptamer and γ-cyclodextrin: A sensitive excimer signaling approach for aptamer biosensors

Cited by 23 publications

References 50 publications

The Design and Improvement of Aptamer-based Fluorescent Probes

The Design and Improvement of Aptamer-based Fluorescent Probes

Rationally designed molecular beacons for bioanalytical and biomedical applications

Fabrication of β-cyclodextrin modified mesostructured silica coated multi-walled carbon nanotubes composites and application for paraben removal

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