Molekular-zytogenetische Analysen an alter DNA (aDNA) anhand von Präparaten der Meckelschen Sammlungen zu Halle (Saale)

Tönnies, Holger; Klunker, Rudyard; Saar, Kathrin; Göbbel, Luminita; Musil, A; Schultka, Rüdiger

doi:10.1016/s0940-9602(02)80094-2

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…A research project was started, in which molecular cytogenetic techniques are used in combination with radiographical techniques, computer tomography (CT), spiral CT, and magnetic resonance imaging (MRI) to diagnose such anomalies. Umbilical cord biopsies were taken to test for chromosome imbalances, which could have caused the malformations [Tönnies et al, 2002, 2005]. The re‐examination and diagnosis of the human fetuses provides a unique opportunity to present important specimens from the Meckel Collection investigated with modern techniques.…”

Section: Introductionmentioning

confidence: 99%

Improvement of bonding strength between biomimetic apatite coating and substrate

Wei

2007

J Biomed Mater Res

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Bone-like apatite coatings were prepared using a biomimetic method in a modified simulated body fluid (m-SBF). The effect of the m-SBF volume on the apatite coating quality was studied. Three m-SBF volumes, 50, 100, and 200 mL, were employed to immerse titanium substrates in a sealed container so as to produce apatite coatings with different properties, namely types I, type II, and type III apatite coatings, respectively. The coatings were characterized using X-ray diffraction and environmental scanning electron microscope. The bonding between the coating and the Ti substrate was evaluated using an adhesive strength test. All three apatite coatings demonstrated a poorly crystallized structure, and the coatings formed exhibited a uniformed surface morphology. Further increasing the m-SBF volume, small globules of apatite started to form on the surface of the coating. The bonding strength for the three coating systems were 8.52 +/- 2.41, 10.36 +/- 2.78, and 17.23 +/- 2.55 MPa for types I, II, and III apatite coatings, respectively. The failure analyses suggested that type III coating failed mostly at the interface between the coating and the substrate, while type I and II coatings failed mostly within the apatite coating. Our study revealed that a dense, thick, well-adhered apatite coating could be achieved by carefully controlling the volume of m-SBF.

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Section: Introductionmentioning

confidence: 99%

Improvement of bonding strength between biomimetic apatite coating and substrate

Wei

2007

J Biomed Mater Res

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“…In the last few years, the collection, and in particular the anomalous human and animal fetuses were studied to identify the specimens described by Meckel the Younger and his students [Klunker, 2003]. DNA‐based molecular cytogenetic techniques, such as comparative genomic hybridization (CGH), were used in combination with radiographic techniques, computer tomography (CT), spiral CT, and magnetic resonance imaging (MRI) to reassess the diagnoses from a contemporary perspective and to compare these with the original diagnoses [Klunker et al, 2002, 2005; Tönnies et al, 2002, 2005; Göbbel et al, 2005]. The collection includes many malformations which Meckel the Younger called either anomalies of incomplete development or primary malformations ( die ursprünglichen Bildungsfehler ).…”

Section: Introductionmentioning

confidence: 99%

Nuchal cystic hygroma in five fetuses from 1819 to 1826 in the Meckel‐anatomical collections at the University of Halle, Germany

Göbbel¹,

Schultka²,

Klunker³

et al. 2006

American J of Med Genetics Pt A

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The Anatomical collection of the Department of Anatomy and Cell Biology, Medical School of the University of Halle, Germany, comprises more than 8,000 specimens. Around 600 of them show congenital anomalies. The collection of abnormal human and animal fetuses began as the private collection of Johann Friedrich Meckel the Elder (1724-1774), his son Philipp Friedrich Theodor Meckel (1755-1803) and his grandson Johann Friedrich Meckel the Younger (1781-1833). Meckel the Younger founded the systematic science of developmental pathology. Radiographical techniques, computer tomographic (CT) methods, magnetic resonance imaging (MRI), and comparative genomic hybridization (CGH) were used to diagnose abnormal human fetuses in the Meckel-anatomical collections. Cystic hygroma colli was found in five of the human fetuses originally described by JF Meckel the Younger in 1826 and one of his students in 1819 [Hencke, 1819]. CGH analyses were used to test whether the observed cystic hygroma colli could be caused by chromosomal aneuploidies. CGH-ratio profiles of all chromosomes were apparently normal. PCR-based sex determination tests on ancient DNA were used to determine the fetal gonosomal constitution. It is likely that the Meckel specimens are among the oldest fetuses in which Ullrich-Turner "phenotype" has been diagnosed.

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Acrofacial dysostosis (AFD) with preaxial limb hypoplasia (Nager AFD) and club foot diagnosed in a fetus from 1812 in the anatomical collections at the University of Halle, Germany

Göbbel

Schultka

Klunker

et al. 2005

American J of Med Genetics Pt A

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The Anatomical Collections of the Department of Anatomy and Cell Biology at the University of Halle, Germany, comprise more than 8,000 specimens, about 600 of them congenital anomalies. The collection of abnormal human and animal specimens began with the private collections of Johann Friedrich Meckel the Elder (1724–1774), his son Philipp Friedrich Theodor Meckel (1755–1803), and his grandson Johann Friedrich Meckel the Younger (1781–1833). Meckel the Younger founded the science of developmental pathology in Germany. Radiographical techniques, computer tomographic methods (CT), magnetic resonance imaging (MRI), and molecular cytogenetic techniques, for example, comparative genomic hybridization (CGH) were used to diagnose abnormal human fetuses in the Meckel Collection. On examination of one of the human fetuses, originally described by JF Meckel the Younger in 1812 or earlier, we found striking clinical manifestations including mandibulofacial defects and preaxially malformed limbs. With respect to external findings, we propose that the condition is acrofacial dysostosis (AFD) with preaxial limb hypoplasia (Nager AFD) in combination with club foot, tibial torsion, and single umbilical artery. We used genetic analyses to test whether the observed limb malformations could be caused by aneuploidy. CGH‐ratio profiles of all chromosomes were apparently normal. It is likely that Meckel's specimen is the earliest known fetus with Nager AFD. © 2005 Wiley‐Liss, Inc.

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Molekular-zytogenetische Analysen an alter DNA (aDNA) anhand von Präparaten der Meckelschen Sammlungen zu Halle (Saale)

Cited by 4 publications

References 12 publications

Improvement of bonding strength between biomimetic apatite coating and substrate

Improvement of bonding strength between biomimetic apatite coating and substrate

Nuchal cystic hygroma in five fetuses from 1819 to 1826 in the Meckel‐anatomical collections at the University of Halle, Germany

Acrofacial dysostosis (AFD) with preaxial limb hypoplasia (Nager AFD) and club foot diagnosed in a fetus from 1812 in the anatomical collections at the University of Halle, Germany

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