Molekulargewichtsbezogene Analyse der physiologischen Proteinurie Neugeborener

Thanner, F.; Wartha, R.; Gekle, D

doi:10.1007/bf01476510

Cited by 4 publications

(4 citation statements)

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“…The results obtained by analysis of the molecular weights of urinary proteins in healthy newborns, separated by SDS-PAGE and stained with Coomassie Brilliant Blue G-250, reported by Thanner et al (23) confirmed the findings of previous mentioned authors for presence of glomerulo-tubular imbalance in early life. According to the number of separated protein fractions and their relative concentration (expressed as percent of total protein concentration) they concluded that proteinuria in healthy full-term neonates is predominantly tubular and is due to ineffective proximal tubular reabsorption of low molecular weight microproteins.…”

Section: Resultssupporting

confidence: 88%

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Evaluation of urinary proteins in healty full-term neonates by SDS-PAGE

Palchevska¹,

Tasik²,

Korneti³

et al. 2005

Maced. Pharm. Bull.

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The pattern of urinary proteins in healthy full-term neonates was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), coupled with determination of few parameters related to urinary protein excretion. Twenty healthy full-term neonates were included in the investigation. Five urine samples from each subject were collected on days 3, 7, 14, 21 and 28 after birth. Determination of total proteins was performed using turbidimetric method with sulfosalicylic acid. Urinary creatinine concentration was determined by Jaffe method. Urinary proteins were separated by horizontal gradient SDS-PAGE according to Görg. The highest values for total urinary proteins and for protein/creatinine ratio were detected in urine samples excreted on days 3 and 7 after birth. Three types of SDS-PAG electrophoretic profiles were observed. The first type of electrophoretic profile was characterized by the presence of proteins of mixed glomerular and tubular origin with molecular weights from 10 to 160 kDa. Typical for the second type of electrophoretic profile was the presence of two faint fractions with molecular weights of 78 and 90 kDa and several intensive low molecular weight fractions (14-67 kDa). In the third type of electrophoretic profile only low molecular weight proteins (10-67 kDa) were detected in all five urine samples. These findings express the transitory immaturity of the glomerular filter and tubular protein reabsorbing system of the newborn kidney. Apparently, the tubular protein handling normalizes later than the glomerular filtration of proteins.

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Section: Resultssupporting

confidence: 88%

“…There are also a great number of studies related to quantitative changes in some specific urinary proteins during the first month of life and later in early infancy and older children (19,20). There are only a few reports in literature regarding the SDS-PAGE electrophoretic profiles in healthy newborns (21)(22)(23).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of urinary proteins in healty full-term neonates by SDS-PAGE

Palchevska¹,

Tasik²,

Korneti³

et al. 2005

Maced. Pharm. Bull.

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“…Because a great number of proteinuric patients demonstrate not simply glomerular or tubular pattern, the ratio LMW/HMW or any other measure calculated on the basis of the relation between proteins of different size (e.g. glomerulo-tubular protein ratio, Thanner et al 1979) might appear to be normal even though there are distinct changes. Therefore it is important to determine LMW and HMW proteins quantitatively or at least semi-quantitatively in order not to lose information.…”

Section: Characterization Of Urinary Proteinsmentioning

confidence: 99%

“…Several LMW plasma proteins such as lysozyme (Pruzanski and Wilson 1977) or beta-2 microglobuiin (Hall and Vasiljevic 1973) which can easily be measured with a commercially available test kit, have been used as marker molecules. Another frequently applied method to characterize urinary proteins is the electrophoretic separation to demonstrate the molecular weight distribution (Boesken et al 1973;Balant et al 1974;Thanner et al 1979).…”

Section: Introductionmentioning

confidence: 99%

Urinary protein excretion in interstitial and tubular kidney disease as characterized by gradient electrophoresis

et al. 1983

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Summary.In order to estimate the degree of tubular lesions and to detect a possible glomerular involvement, proteinuria was studied in tubular and/ or interstitial nephropathies by determination of total protein and beta-2 microglobulin and by micro-gradientgel electrophoresis. The excretion of albumin, low molecular weight proteins (LMW) and high molecular weight (HMW) proteins was measured in addition. Other proximal tubulus functions such as glucose and phosphate reabsorption were also measured. The clinical entities studied were: chronic postinfectious nephritis, patients with nephropathy following analgesic abuse, tubular glucosuria in pregnancy and cis-platinum treated patients suffering from testicular tumor. The results were interpreted basing on the "selectivity" or urinary protein patterns, which means the relation between LMW and HMW proteins. Micro-gradientgel electrophoresis proved to be a fast and sensitive tool for quantitative as well as qualitative analysis of albumin, LMW and HMW proteins.All patients with advanced tubulo-interstitial disease excreted increased amounts of LMW proteins. The excretion of beta-2 microglobulin was also high and correlated with the LMW protein excretion. The albumin and HMW protein excretion showed no relation to LMW proteins. The disproportionate increase of LMW protein excretion on the one hand and albumin and HMW protein excretion on the other suggests different reabsorption mechanisms. The tubular patterns originate from a more easily disturbed reabsorption of LMW proteins and/or from a normally more active reabsorption of LMW proteins. Except in renal glucosuria phosphate and glucose excretion were not increased. This means that the capacity Offprint requests to: Dr. rer. nat. J. Alt (address see page 648) for the reabsorption of glucose and phosphate copes with a normal filtered load even in patients with highly increased proteinuria of a tubular type.

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Proteinurie

Ehrich¹,

Wurster²

1985

Nephrologie — Urologie

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Molekulargewichtsbezogene Analyse der physiologischen Proteinurie Neugeborener

Cited by 4 publications

References 20 publications

Evaluation of urinary proteins in healty full-term neonates by SDS-PAGE

Evaluation of urinary proteins in healty full-term neonates by SDS-PAGE

Urinary protein excretion in interstitial and tubular kidney disease as characterized by gradient electrophoresis

Proteinurie

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