Molluscan aquaporins: an overview, with some notes on their role in the entry into aestivation in gastropods

Kosicka, Ewa; Lesicki, Andrzej; Pieńkowska, Joanna R.

doi:10.1080/13235818.2020.1716442

Cited by 11 publications

(20 citation statements)

References 62 publications

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“…Aquaporin channels comprise numerous functionally distinct family members. These have been traditionally divided into four major functional groups, AQP1-like, AQP3-like, AQP8-like and AQP11-like types, based on their distinct evolutionary origin [ 76 ]. Previous RNA-Seq studies have successfully used phylogenetic analysis to evaluate the origin of putative aquaporin transcripts identified in a transcriptome (e.g., Calcino et al, 2019 [ 77 ]), which provides greater confidence of gene family member identity, compared to blast sequence similarity searches.…”

Section: Resultsmentioning

confidence: 99%

Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience

Barrett

Thyrring

Harper

et al. 2022

Genes

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Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‰) and low salinities (15‰) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‰, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‰, 15‰ and 5‰) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world.

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Section: Resultsmentioning

confidence: 99%

Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience

Barrett

Thyrring

Harper

et al. 2022

Genes

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“…However, when analyzing the sequences of SIPs, no conserved residues with AQP11 were found, pointing out that the association within the same clade is more likely a long branch artifact rather than a real relationship of orthology [ 13 ]. On the other hand, high variability in the overall Aqp primary structures and substitutions within the NPA motifs is common in invertebrates [ 73 ], thus potentially explaining the great difference of our AsucAQPd compared with the already known vertebrate AQP11 and AQP12. Moreover, other studies have also suggested the presence of orthologs of these vertebrate AQPs in mollusks, arthropods and nematodes [ 28 , 66 , 74 ].…”

Section: Resultsmentioning

confidence: 99%

“…No aquaglyceroporins were recovered in the A. succinea transcriptome; however, their presence was retrieved in the congeneric A. virens (Sars, 1835) , as well as in the other annelids and invertebrates ( Figure 2 and Figure 3 ) [ 66 , 73 , 75 ]. Their presumed absence may be due to low sequencing depth (e.g., only one sample [ 43 ]) which may have resulted in a limited number of genes recovered in the transcriptome.…”

Section: Resultsmentioning

confidence: 99%

Finding Aquaporins in Annelids: An Evolutionary Analysis and a Case Study

Mucciolo

Desiderato

Salonna

et al. 2021

Cells

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Aquaporins (AQPs) are a family of membrane channels facilitating diffusion of water and small solutes into and out of cells. Despite their biological relevance in osmoregulation and ubiquitous distribution throughout metazoans, the presence of AQPs in annelids has been poorly investigated. Here, we searched and annotated Aqp sequences in public genomes and transcriptomes of annelids, inferred their evolutionary relationships through phylogenetic analyses and discussed their putative physiological relevance. We identified a total of 401 Aqp sequences in 27 annelid species, including 367 sequences previously unrecognized as Aqps. Similar to vertebrates, phylogenetic tree reconstructions clustered these annelid Aqps in four clades: AQP1-like, AQP3-like, AQP8-like and AQP11-like. We found no clear indication of the existence of paralogs exclusive to annelids; however, several gene duplications seem to have occurred in the ancestors of some Sedentaria annelid families, mainly in the AQP1-like clade. Three of the six Aqps annotated in Alitta succinea, an estuarine annelid showing high salinity tolerance, were validated by RT-PCR sequencing, and their similarity to human AQPs was investigated at the level of “key” conserved residues and predicted three-dimensional structure. Our results suggest a diversification of the structures and functions of AQPs in Annelida comparable to that observed in other taxa.

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“…Temperature deviations from the normal activity range entail behavioural and physiological defence against thermal stress. However, the physiological processes of the snail entering into torpor are dynamic and it may take a few days to prepare the organism for this metabolic state (KosicKa et al 2020). Therefore, it is likely that V. antivertigo in the experiment did not enter torpor, but remained inactive while still ready to resume activity.…”

Section: Folia Malacol 29(3): 186-189mentioning

confidence: 99%

Impact of a short-time exposure to different temperatures on wakening time of Vertigo antivertigo (Draparnaud, 1801) (Gastropoda: Eupulmonata: Vertiginidae)

Książkiewicz‐Parulska¹

2021

Folia Malacol.

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I checked experimentally how a short-time exposure to different temperatures: 1 °C, 24 °C and 34 °C in a high humidity affects wakening time of Vertigo antivertigo (Draparnaud). The snails were exposed to experimental conditions for 48 hours (each snail in a separate test tube, provided with food and a source of calcium). After that time, all the individuals were transferred to room temperature (24 °C) for counting the time needed for wakening. The shortest wakening time was noted for snails kept at 1 °C, the longest – for snails kept at room temperature of 24 °C.

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Molluscan aquaporins: an overview, with some notes on their role in the entry into aestivation in gastropods

Cited by 11 publications

References 62 publications

Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience

Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience

Finding Aquaporins in Annelids: An Evolutionary Analysis and a Case Study

Impact of a short-time exposure to different temperatures on wakening time of Vertigo antivertigo (Draparnaud, 1801) (Gastropoda: Eupulmonata: Vertiginidae)

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