Molt-inhibiting hormone (MIH) gene from the green mud crab<i>Scylla paramamosain</i>and its expression during the molting and ovarian cycle

Huang, Huiyang; Fu, Chunru; Chen, Xuelei; Gong, Jie; Huang, Xiaoshuai; Ye, Haihui

doi:10.1111/are.12421

Cited by 34 publications

(24 citation statements)

References 42 publications

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“…At temperatures that juvenile M. magister tolerate over prolonged periods (10 and 15°C; Kondzela and Shirley, 1993), Mm-MIH was constitutively expressed over the moult cycle, as expected. A decrease of MIH expression in premoult was reported for Callinectes sapidus and in Scylla paramamosain (Huang et al, 2015;Lee et al, 1998) and observed in our experimental animals at 20°C. Transcription was downregulated at 20°C also in the other genes, but to a lesser extent.…”

Section: Discussionsupporting

confidence: 85%

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

Wittmann

Benrabaa

López-Cerón

et al. 2018

Journal of Experimental Biology

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Mechanistic target of rapamymcin (mTOR) is a highly conserved protein kinase that controls cellular protein synthesis and energy homeostasis. We hypothesize that mTOR integrates intrinsic signals (moulting hormones) and extrinsic signals (thermal stress) to regulate moulting and growth in decapod crustaceans. The effects of temperature on survival, moulting and mRNA levels of mTOR signalling genes (, ,, and) and neuropeptides ( and ) were quantified in juvenile Crabs at different moult stages (12, 19 or 26 days postmoult) were transferred from ambient temperature (∼15°C) to temperatures between 5 and 30°C for up to 14 days. Survival was 97-100% from 5 to 20°C, but none survived at 25 or 30°C. Moult stage progression accelerated from 5 to 15°C, but did not accelerate further at 20°C. In eyestalk ganglia, , and mRNA levels decreased with increasing temperatures. and mRNA levels were lowest in the eyestalk ganglia of mid-premoult animals at 20°C. In the Y-organ, mRNA levels decreased with increasing temperature and increased during premoult, and were positively correlated with haemolymph ecdysteroid titre. In the heart, moult stage had no effect on mTOR signalling gene mRNA levels; only , and mRNA levels were higher in intermoult animals at 10°C. These data suggest that temperature compensation of neuropeptide and mTOR signalling gene expression in the eyestalk ganglia and Y-organ contributes to regulate moulting in the 10 to 20°C range. The limited warm compensation in the heart may contribute to mortality at temperatures above 20°C.

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Section: Discussionsupporting

confidence: 85%

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

Wittmann

Benrabaa

López-Cerón

et al. 2018

Journal of Experimental Biology

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“…During the ovarian development of S. paramamosain , the expression levels of Sp‐CHH increased significantly from stage I to stage IV, when the ovary develops from an undeveloped stage and go through stages of active vitellogenesis and oocyte maturation, and declined at stage V, when vitellogenesis has completed (Huang Ye, et al . ). These results strongly suggested that Sp‐CHH might be concerned with vitellogenesis in the mud crab.…”

Section: Discussionmentioning

confidence: 97%

“…A β ‐actin fragment was amplified using the primer pair, Actinf and Actinr (Table ), and served as the internal control (Huang, Ye, et al . ). The PCR conditions were as follows: an initial denaturation (1 min, 95°C), 45 cycles of denaturation (30 s, 95°C), annealing (30 s, 55°C), and extension (40 s, 72°C).…”

Section: Methodsmentioning

confidence: 97%

“…The moulting cycle in the mud crab can be classified into three stages: postmoult (soft shell), intermoult (hard shell) and premoult (double shell) stages, according to the characteristics of the pleopods (Kuo & Lin ; Huang, Fu, Chen, Gong, Huang & Ye ). The ovarian development can be divided into five stages: stage I (undeveloped stage), stage II (previtellogenic stage), stage III (early vitellogenic stage), stage IV (late vitellogenic stage) and stage V (mature stage) (Shangguan, Liu & Li ; Huang, Ye, Huang, Yang & Gong ).…”

Section: Methodsmentioning

confidence: 99%

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Crustacean hyperglycaemic hormone gene from the mud crab,Scylla paramamosain: cloning, distribution and expression profiles during the moulting cycle and ovarian development

Huang

Gong

et al. 2014

Aquac Res

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Recently, crustacean hyperglycaemic hormone (CHH) has gained wide attention for its diverse physiological functions. In this study, two cDNA sequences encoding CHH, Sp-CHH1 and Sp-CHH2, were cloned from the eyestalk ganglia of the mud crab, Scylla paramamosain. Each conceptually translated precursor is expected to be processed into the signal peptide, the crustacean hyperglycaemic hormone precursor-related peptide (CPRP) and the CHH mature peptide. Sp-CHH1 and Sp-CHH2 share an identical sequence for the first 40 residues from the amino-terminus of the peptides, but substantially different from each other in the C-terminus. Quantitative real-time PCR (qPCR) showed that Sp-CHH transcripts were mainly detected in the eyestalk ganglia and to a much lesser extent in the brain, thoracic ganglia, stomach, hepatopancreas, heart and muscle. RNA in situ hybridization in the eyestalk ganglia indicated that the Sp-CHH was localized in the perikarya of neuroendocrine cells belonging to the X-organ of the medulla terminalis. Moreover, Sp-CHH transcripts were stage-specific changed during the moulting cycle, as it increased significantly from postmoult stage to intermoult stage, then decreased sharply at premoult stage. Sp-CHH expression levels in the eyestalk ganglia were also examined at different ovarian stages (stage I to stage V). It was found that the expression was gradually increased from stage I to stage IV, and then it was decreased at stage V. All the findings together suggested that Sp-CHH should be pleiotropic, which might inhibit the synthesis of ecdysteroids by Y-organs and be involved in promoting vitellogenesis in the mud crab.

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“…NPF and NPY belong to NPY family are neuropeptide hormone that accelerates ovarian maturation in female Schistocerca gregaria (SCHOOFS et al 2001), and plays a role in the regulation of visceromotor functions during egg laying (RAJPARA et al 1992). CHH , MIH have long been considered to inhibit crustacean molting, and after E. sinensis finish their last reproductive molting like insects, they will stop molting and initiate their gonad development (CHAN et al 2003; HUIYANG et al 2015). Therefore, extremely highly expressed CHH , MIH in eyestalk may inhibit the molting and induce precocity.…”

Section: Discussionmentioning

confidence: 99%

Tissue-expression profiles unveils the gene interaction of hepatopancreas, eyestalk, and ovary in precocious female Chinese mitten crab,Eriocheir sinensis

Chen

Hou

Yue

et al. 2018

Preprint

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Sexual precocity is a serious and common biological phenomenon in animal species. Large amount of precocity individuals was identified in Chinese mitten crab, Eriocheir sinensis, which caused huge economical loss every year. However, the underlying genetic basis of precocity in E. sinensis is still lack. In this study, histology observation, comparative transcriptome was conducted among different stages of precocious one-year old and normal two-year old E. sinensis, tissue-expression profiles of ovary, hepatopancreas, and eyestalk tissues were presented and compared. Genes associated with lipid metabolic process, lipid transport, vitelline membrane formation, vitelline synthesis and neuropeptide hormone related genes were upregulated in the ovary, hepatopancreas and eyestalk of precocious E. sinensis. Our results indicated eyestalk involved in neuroendocrine system providing neuropeptide hormone that may induce vitellogenesis in hepatopancreas and further stimulate ovary development. Hepatopancreas is a site for energy storage, vitellogenin synthesis and may assist to induce oogenesis through lipid transport in precocious E. sinensis. The genetic basis of precocity in E. sinensis is an integrated gene regulatory network of eyestalk, hepatopancreas, and ovary tissues. Our study provides effective convenient phenotype measurement method for identification of potential precocious E. sinensis detection, and valuable genetic resources and novel insights into the research of molecular mechanism of precocity in E. sinensis.

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Molt-inhibiting hormone (MIH) gene from the green mud crabScylla paramamosainand its expression during the molting and ovarian cycle

Cited by 34 publications

References 42 publications

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

Crustacean hyperglycaemic hormone gene from the mud crab,Scylla paramamosain: cloning, distribution and expression profiles during the moulting cycle and ovarian development

Tissue-expression profiles unveils the gene interaction of hepatopancreas, eyestalk, and ovary in precocious female Chinese mitten crab,Eriocheir sinensis

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