Molybdate-reducing and SDS-degrading Enterobacter sp. Strain Neni-13

Rahman, Mohd Fadhil; Rusnam, M; Gusmanizar, Neni; Masdor, Noor Azlina; Lee, Ching Hao; Shukor, Mohd Shukri; Roslan, Muhamad Akhmal Hakim; Shukor, Mohd Yunus

doi:10.1515/nbec-2016-0017

Cited by 15 publications

(26 citation statements)

References 28 publications

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“…There are various reports describing the isolation of bacteria having the capacity to degrade surfactants, including a wide variety of Pseudomonas strains ( Klebensberger et al, 2006 ; Chaturvedi and Kumar, 2011a ; Yeldho et al, 2011 ; John et al, 2015 ), Klebsiella oxytoca ( Shukor et al, 2009 ; Masdor et al, 2015 ), Enterobacter sp. strain NENI-13 ( Rahman et al, 2016 ), and finally microbial consortia composed of Acinetobacter calcoaceticus and Pantoea agglomerans ( Abboud et al, 2007 ). However, these microorganisms were selected from environments highly contaminated solely with detergents with no other xenobiotics present.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Pseudomonas spp. Strains That Efficiently Decompose Sodium Dodecyl Sulfate

et al. 2017

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Due to their particular properties, detergents are widely used in household cleaning products, cosmetics, pharmaceuticals, and in agriculture as adjuvants tailoring the features of pesticides or other crop protection agents. The continuously growing use of these various products means that water soluble detergents have become one of the most problematic groups of pollutants for the aquatic and terrestrial environments. Thus it is important to identify bacteria having the ability to survive in the presence of large quantities of detergent and efficiently decompose it to non-surface active compounds. In this study, we used peaty soil sampled from a surface flow constructed wetland in a wastewater treatment plant to isolate bacteria that degrade sodium dodecyl sulfate (SDS). We identified and initially characterized 36 Pseudomonas spp. strains that varied significantly in their ability to use SDS as their sole carbon source. Five isolates having the closest taxonomic relationship to the Pseudomonas jessenii subgroup appeared to be the most efficient SDS degraders, decomposing from 80 to 100% of the SDS present in an initial concentration 1 g/L in less than 24 h. These isolates exhibited significant differences in degree of SDS degradation, their resistance to high detergent concentration (ranging from 2.5 g/L up to 10 g/L or higher), and in chemotaxis toward SDS on a plate test. Mass spectrometry revealed several SDS degradation products, 1-dodecanol being dominant; however, traces of dodecanal, 2-dodecanol, and 3-dodecanol were also observed, but no dodecanoic acid. Native polyacrylamide gel electrophoresis zymography revealed that all of the selected isolates possessed alkylsulfatase-like activity. Three isolates, AP3_10, AP3_20, and AP3_22, showed a single band on native PAGE zymography, that could be the result of alkylsulfatase activity, whereas for isolates AP3_16 and AP3_19 two bands were observed. Moreover, the AP3_22 strain exhibited a band in presence of both glucose and SDS, whereas in other isolates, the band was visible solely in presence of detergent in the culture medium. This suggests that these microorganisms isolated from peaty soil exhibit exceptional capabilities to survive in, and break down SDS, and they should be considered as a valuable source of biotechnological tools for future bioremediation and industrial applications.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Pseudomonas spp. Strains That Efficiently Decompose Sodium Dodecyl Sulfate

et al. 2017

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“…8,17,37 Sulfurospirillum are sulfur-oxidizing bacteria that can outcompete sulfate-reducing bacteria for sulfate in the presence of nitrate, thereby reducing H 2 S production. 9,46 Similarly, a few Enterobacteriaceae have been found to reduce molybdate, 51,52 which may have provided a similar competitive advantage to these microbes. At the same time, molybdate is structurally similar to sulfate and inhibits the growth of H 2 S producers, 50 were present only at several timepoints in the nitrate microcosms.…”

Section: Mineral Supplements Control Metabolism and Shape Microbiome ...mentioning

confidence: 99%

Top‐down enrichment of oil field microbiomes to limit souring and control oil composition during extraction operations

et al. 2022

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Microbial processes sour oil, corrode equipment, and degrade hydrocarbons at an annual global cost to the oil and gas industry of nearly $2 billion. However, top-down control of these microbial processes can reduce their damage and enhance oil recovery. Here, we screened microbial communities from five oil wells in the Illinois basin and evaluated nutrient injection strategies to control metabolism and community composition. Molasses with molybdate supplementation stimulated gas and organic acid production while suppressing corrosive H 2 S formation in samples from two wells. These changes were accompanied with significant reshaping of the microbiome community. Simulations of field operations via a lab-scale mini-coreflood validated that oil well microbiomes can be engineered to inhibit deleterious H 2 S and shape oil hydrocarbon composition in situ. These pilot studies validate the economic potential and sustainability of top-down approaches for microbiome engineering to control microbes in oil extraction and enhance the economic viability of oil recovery.comprehensive 2D-gas chromatography, cultivation screening, microbial enhanced oil recovery, microbiome engineering, miniature coreflood, top-down design | BACKGROUNDFossil fuels are likely to remain a significant energy source for at least the next 30 years 1 as economies transition globally toward more renewable energy sources. 2 Extracting trapped oil from aging wells can not only increase the overall production of existing wells but also minimize the need to drill new wells and, ultimately, decrease the cost of extracting oil from existing reservoirs. 3 Expensive polymer and/or surfactant/polymer formulations that require large amounts of funding and research effort are used to extract oil not recovered by primary extraction techniques. 4 Additionally, native and exogenous microbes have been harnessed as a more cost-effective 5 alternative for secondary and tertiary oil recovery-an approach coined microbial enhanced oil recovery (MEOR). 6 However, microbial activity in oil wells, can also be disruptive to oil recovery and/or alter its composition. 6 For example, microbes can degrade and sour oil, and produce metabolites that corrode the well casing, flowlines, and pipelines. 7

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“…Studies on the effect of heavy metal to the growth of the bacterium on SDS utilized the microtiter plate format [32,33]. The growth medium was as follows: Na2HPO4, (1.39 g l -1 ), KNO3, (0.5 g l -1 ), KH2PO4, (1.36 g l -1 ),MgSO4 (0.01 g l -1 ), CaCl2 (0.01 g l -1 ), and (NH4)2SO4 (7.7 g l -1 ) [29]. Then, SDS was filter sterilized using a filter syringe (0.2 µm) and added into the cooled medium to the final concentration of 1000 mg/L.…”

Section: Growth Of Sds-degrading Bacteriummentioning

confidence: 99%

“…There are few studies available that examine the effect of heavy metals on microbial growth, as most studies on this topic use primary models rather than secondary models. In this work, an SDS-degrading bacterium was isolated and it was found that heavy metals including mercury, silver, and mercury significantly inhibited its growth [29,30]. Using various inhibition models, the aim of this study was to investigate the effect of mercury on the growth rate of this bacterium when grown on SDS.…”

Section: Introductionmentioning

confidence: 99%

Mathematical Modelling on the effect of Mercury on the Growth Rate of Serratia marcescens strain DRY6 on Sodium Dodecyl Sulphate

Abubakar

Yakasai

2022

Bull Environ Sci Sust Manage

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Sodium dodecyl sulfate (SDS) or sodium lauryl sulfate (SLS) is a common anionic surfactant found in various cleaning and personal hygiene products. It has both a polar "headgroup" and a hydrocarbon tail, giving it amphiphilic properties that make it effective as a detergent. However, this also makes it a major pollutant in aquatic environments. Researchers have studied the biodegradation of SDS by microorganisms, particularly bacteria, as a potential cleanup method. It has been found that mercury can significantly inhibit the degradation of SDS by the Serratia marcescens strain DRY6 bacteria. At different mercury concentrations, the bacteria exhibited sigmoidal growth with lag times of 7 to 10 hours, but overall growth was decreased with higher mercury concentrations, with a concentration of 1.0 g/L virtually stopping all growth. A modified Gompertz model was used to calculate growth rates at various mercury concentrations, and these rates were then modeled using five different models: modified Han-Levenspiel, Wang, Liu, modified Andrews, and Amor. Only three of the models (Wang, modified Han-Levenspiel, and Liu) were able to accurately fit the curve, with the Wang model performing the best statistically. The Wang model yielded estimates of 0.216 (95% confidence interval: 0.193 to 0.239) for the critical heavy metal ion concentration, 1.05 (95% confidence interval: 0.938 to 1.167) for the maximum growth rate, and 0.389 (95% confidence interval: 0.148 to 0.636) for the empirical constant , represented by Ccrit, max and m, respectively.

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Molybdate-reducing and SDS-degrading Enterobacter sp. Strain Neni-13

Cited by 15 publications

References 28 publications

Isolation and Characterization of Pseudomonas spp. Strains That Efficiently Decompose Sodium Dodecyl Sulfate

Isolation and Characterization of Pseudomonas spp. Strains That Efficiently Decompose Sodium Dodecyl Sulfate

Top‐down enrichment of oil field microbiomes to limit souring and control oil composition during extraction operations

Mathematical Modelling on the effect of Mercury on the Growth Rate of Serratia marcescens strain DRY6 on Sodium Dodecyl Sulphate

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