Molybdenum‐ and tungsten‐containing formate dehydrogenases and formylmethanofuran dehydrogenases: Structure, mechanism, and cofactor insertion

Niks, Dimitri; Hille, Russ

doi:10.1002/pro.3498

Cited by 99 publications

(97 citation statements)

References 60 publications

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“…In contrast to recent reports on the heterologously expressed R. capsulatus enzyme and the FDH from C. necator, FdsD is retained as a subunit in the active enzyme ( Supplementary Fig. 1c) 6,16,19 . Earlier analyses also support a FdsABGD complex arrangement 20,21 , indicating that likely all NAD + -dependent FDHs have a similar overall subunit composition.…”

Section: Resultscontrasting

confidence: 89%

Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase

et al. 2020

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Metal-containing formate dehydrogenases (FDH) catalyse the reversible oxidation of formate to carbon dioxide at their molybdenum or tungsten active site. They display a diverse subunit and cofactor composition, but structural information on these enzymes is limited. Here we report the cryo-electron microscopic structures of the soluble Rhodobacter capsulatus FDH (RcFDH) as isolated and in the presence of reduced nicotinamide adenine dinucleotide (NADH). RcFDH assembles into a 360 kDa dimer of heterotetramers revealing a putative interconnection of electron pathway chains. In the presence of NADH, the RcFDH structure shows charging of cofactors, indicative of an increased electron load.

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Section: Resultscontrasting

confidence: 89%

Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase

et al. 2020

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“…Three externally oriented formate dehydrogenases fdoIGH-fdhA [47,48] were encoded, one of which was actively expressed. Each of these gene clusters encoded a membrane-bound b-type cytochrome, which transfers the electrons from MKH 2 to an Fe-S protein, the catalytic subunit of the formate dehydrogenases, and generates formate in the periplasm, similar as that described for S. wolfei [7,49].…”

Section: H 2 /Formate Production and Other Confurcation/bifurcation Mmentioning

confidence: 99%

Novel syntrophic bacteria in full-scale anaerobic digesters revealed by genome-centric metatranscriptomics

et al. 2020

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Short-chain fatty acid (SCFA) degradation is an important process in methanogenic ecosystems, and is usually catalyzed by SCFA-oxidizing bacteria in syntrophy with methanogens. Current knowledge of this functional guild is mainly based on isolates or enrichment cultures, but these may not reflect the true diversity and in situ activities of the syntrophs predominating in full-scale systems. Here we obtained 182 medium to high quality metagenome-assembled genomes (MAGs) from the microbiome of two full-scale anaerobic digesters. The transcriptomic response of individual MAG was studied after stimulation with low concentrations of acetate, propionate, or butyrate, separately. The most pronounced response to butyrate was observed for two MAGs of the recently described genus Candidatus Phosphitivorax (phylum Desulfobacterota), expressing a butyrate beta-oxidation pathway. For propionate, the largest response was observed for an MAG of a novel genus in the family Pelotomaculaceae, transcribing a methylmalonyl-CoA pathway. All three species were common in anaerobic digesters at Danish wastewater treatment plants as shown by amplicon analysis, and this is the first time their syntrophic features involved in SCFA oxidation were revealed with transcriptomic evidence. Further, they also possessed unique genomic features undescribed in well-characterized syntrophs, including the metabolic pathways for phosphite oxidation, nitrite and sulfate reduction.

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“…NAD-independent FDH is characterized by high activity toward CO 2 and sensitivity to oxygen (Maia et al, 2016;Yu et al, 2017). This class of FDH contains complex redox-active centers harboring different transition metals, such as molybdenum (Mo), tungsten and nonhaemiron, molybdopterin guanine dinucleotide (MGD) and selenocysterine (Niks and Hille, 2019;Lemaire et al, 2020). It has been identified only in prokaryotic organisms.…”

Section: Introduction Of Synthetic Co 2 Fixation Pathways Into Heteromentioning

confidence: 99%

Recent Advances in Developing Artificial Autotrophic Microorganism for Reinforcing CO2 Fixation

Liang

Zhao²,

Jian-ming

2020

Front. Microbiol.

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With the goal of achieving carbon sequestration, emission reduction and cleaner production, biological methods have been employed to convert carbon dioxide (CO2) into fuels and chemicals. However, natural autotrophic organisms are not suitable cell factories due to their poor carbon fixation efficiency and poor growth rate. Heterotrophic microorganisms are promising candidates, since they have been proven to be efficient biofuel and chemical production chassis. This review first briefly summarizes six naturally occurring CO2 fixation pathways, and then focuses on recent advances in artificially designing efficient CO2 fixation pathways. Moreover, this review discusses the transformation of heterotrophic microorganisms into hemiautotrophic microorganisms and delves further into fully autotrophic microorganisms (artificial autotrophy) by use of synthetic biological tools and strategies. Rapid developments in artificial autotrophy have laid a solid foundation for the development of efficient carbon fixation cell factories. Finally, this review highlights future directions toward large-scale applications. Artificial autotrophic microbial cell factories need further improvements in terms of CO2 fixation pathways, reducing power supply, compartmentalization and host selection.

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Molybdenum‐ and tungsten‐containing formate dehydrogenases and formylmethanofuran dehydrogenases: Structure, mechanism, and cofactor insertion

Cited by 99 publications

References 60 publications

Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase

Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase

Novel syntrophic bacteria in full-scale anaerobic digesters revealed by genome-centric metatranscriptomics

Recent Advances in Developing Artificial Autotrophic Microorganism for Reinforcing CO2 Fixation

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