Molybdenum-containing polypyrrole self-supporting hollow flexible electrode for hydrogen peroxide detection in living cells

Cao, Pengfei; Wang, Nan; Dai, Hongxiu; Ma, Houyi; Lin, Meng

doi:10.1016/j.aca.2021.338251

Cited by 16 publications

(8 citation statements)

References 42 publications

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“…As the key element of electrochemical biosensors, the sensing electrode that was directly in contact with target biomolecules plays key roles in determining the detection sensitivity and stability. − The sensing electrodes with high electrical conductivity and mechanical flexibility could enable efficient and stable electrochemical signal conduction even under mechanical deformations, thus ensuring detection sensitivity and stability. − One-dimensional (1D) nanostructures, such as nanowires and nanotubes, are supposed to offer straightforward nanochannels for fast electron transport, promising their excellent electrical conductivity. , Besides, 1D nanostructures could be cross-linked into two-dimensional (2D) network structured electrodes with low contact resistance, thus offering an electrical conduction pathway to achieve high sensitivity in clinical POCT applications. , More importantly, the interconnected network structure could transfer loading strain effectively under large bending deformation to ensure the mechanical integrity and electrical conductivity of the whole structure. , In this regard, 2D flexible network structured electrodes that were cross-linked by conductive 1D nanowires might offer a pathway to achieve high sensitivity and stability point-of-care detection of complex clinical biological samples.…”

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confidence: 99%

Flexible Point-of-Care Electrodes for Ultrasensitive Detection of Bladder Tumor-Relevant miRNA in Urine

Chen

Liu

et al. 2023

Anal. Chem.

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Portable point-of-care testing (POCT) is currently drawing enormous attention owing to its great potential for disease diagnosis and personal health management. Electrochemical biosensors, with the intrinsic advantages of cost-effectiveness, fast response, ease of miniaturization, and integration, are considered as one of the most promising candidates for POCT application. However, the clinical application of electrochemical biosensors-based POCT is hindered by the decreased detection sensitivity due to the low abundance of disease-relevant biomolecules in extremely complex biological samples. Herein, we construct a flexible electrochemical biosensor based on single-stranded DNA functionalized single-walled carbon nanotubes (ssDNA-SWNTs) for high sensitivity and stability detection of miRNA-21 in human urine to achieve bladder cancer (BCa) diagnosis and classification. The ssDNA-SWNT electrodes with a 2D interconnected network structure exhibit a high electrical conductivity, thus enabling the ultrasensitive detection of miRNA-21 with a detection limit of 3.0 fM. Additionally, the intrinsic flexibility of ssDNA-SWNT electrodes endows the biosensors with the capability to achieve high stability detection of miRNA-21 even under large bending deformations. In a cohort of 40 BCa patients at stages I−III and 44 negative control samples, the constructed ssDNA-SWNT biosensors could detect BCa with a 92.5% sensitivity, an 88.6% specificity, and classify the cancer stages with an overall accuracy of 81.0%. Additionally, the flexible ssDNA-SWNT biosensors could also be utilized for treatment efficiency assessment and cancer recurrence monitoring. Owing to their excellent sensitivity and stability, the designed flexible ssDNA-SWNT biosensors in this work propose a strategy to realize point-of-care detection of complex clinical samples to achieve personalized healthcare.

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confidence: 99%

Flexible Point-of-Care Electrodes for Ultrasensitive Detection of Bladder Tumor-Relevant miRNA in Urine

Chen

Liu

et al. 2023

Anal. Chem.

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“…Ascorbic acid was chosen as a stimulant agent due to its prooxidation property in high concentration. [40][41][42] As shown in Figure 10, the absorbance at 652 nm of other groups was very low, while the corresponding absorbance of Pd-LNT NPs group was 0.270 (2 × 10 5 cells) and 0.478 (4 × 10 5 cells), respectively. It was obvious that when the number of cells increased, the absorbance gradually increased.…”

Section: Chemistryselectmentioning

confidence: 94%

Palladium Nanoparticles Stabilized by Lentinan with Enhanced Peroxidase‐like Activity for Sensitive Detection of H₂O₂

Dong

Liang

et al. 2022

ChemistrySelect

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Natural enzymes are special proteins with excellent catalytic activity and selectivity. It is difficult to purify, store without losing the catalytic ability. Therefore, it is of great significance to study mimetic enzymes to realize the functions of natural enzymes and overcome their shortcoming. Herein, lentinan was used as a biological template to prepare palladium nanoparticles stabilized by lentinan (Pd‐LNT NPs), whose structure, activity and function were characterized. In addition, the 3,3′,5,5′‐tetramethylbenzidine (TMB) was oxidized by Pd‐LNT NPs in the presence of H2O2, which proved that they had good peroxidase‐like activity. The optimum reaction conditions were 30 °C and pH 4, the catalytic kinetic parameters were consistent with Michaelis‐Menten equation, and the good catalytic performance was verified. Furthermore, the colorimetric reaction based on the good peroxidase‐like activity of Pd‐LNT NPs was used for the convenient detection of H2O2. The linear range of colorimetric detection of H2O2 was 3.571‐107.143 μM, and it is worth noting that the detection limit was low to 4.714 μM. At last, Pd‐LNT NPs as biomimetic enzymes were used to detect the H2O2 released from HeLa cells, indicating they have great potential in the field of biological application.

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“…By introducing polymerized PPy particles into Ti 3 C 2 -MXene composite films, Fan et al synthesized PPy-Ti 3 C 2 T x nanosheets to avoid restacking of MXene materials, which were used as supercapacitor electrodes, showing potential for high-performance and wide-temperature applications . A petal MoO 3 –MoS 2 supported by PPy was prepared as a flexible electrode in living cells for nonenzymatic detection of H 2 O 2 , exhibiting a high electrochemical sensing performance . Li et al provided a mechanistic interpretation on how PPy improves the stability of the composite by coating on LiFePO 4 that suppresses the dissolution of Fe and stabilizes the olivine structure and its solid electrolyte interphase (SEI), highly improving the half-cell cycling stability .…”

Section: Introductionmentioning

confidence: 99%

“…34 A petal MoO 3 −MoS 2 supported by PPy was prepared as a flexible electrode in living cells for nonenzymatic detection of H 2 O 2 , exhibiting a high electrochemical sensing performance. 35 Li et al provided a mechanistic interpretation on how PPy improves the stability of the composite by coating on LiFePO 4 that suppresses the dissolution of Fe and stabilizes the olivine structure and its solid electrolyte interphase (SEI), highly improving the half-cell cycling stability. 36 Although progress has been reported in PPy and LiFePO 4 composite electrodes, to acquire high capacity, pre-eminent rate capability, and superior long steadiness, the synergy and symbiosis mechanism of PPy and C-LiFePO 4 still needs to be further explored.…”

mentioning

confidence: 99%

Synergy and Symbiosis Analysis of Capacity-Contributing Polypyrrole and Carbon-Coated Lithium Iron Phosphate Nanostructures for High-Performance Cathode Materials

Zhen

Wang

et al. 2023

ACS Appl. Nano Mater.

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To address the existing problems of commercial inorganic cathodes, including relatively low capacity, poor rate performance, structural instability, and low conductivity, it is critical to introduce a conductive matrix accompanied with electrochemical activity. Conductive polymers have great potential as electrodes with good conductivity, high redox activity, and potential. In this study, carbon-coated lithium iron phosphate (C-LiFePO4) nanoparticles were effectively dispersed in a polypyrrole (PPy) matrix by in situ pulverization. PPy, as an active nanostructure, significantly improves conductivity and accelerates Li+ diffusion. To further explore the synergy and symbiosis mechanism of PPy and C-LiFePO4 (hereinafter called C-LFP in the composite), the nanoparticle dispersion, carburization dependence, and heat treatment preference were investigated. Therefore, a reasonable amount of PPy (25 wt %) hybridization, a moderately wrapped carbon buffer layer (5.3 wt %), and a suitable heat treatment (100 °C) were employed to prepare the (C-LFP)0.75(PPy)0.25 nanocomposite. With a smaller particle size, uniformly dispersed morphology, and good synergy effect between PPy and C-LiFePO4, (C-LFP)0.75(PPy)0.25 delivers a high discharge capacity (209.1 mAh g–1 at 0.1C), a superior rate capability (86.1 mAh g–1 at 10C), and an outstanding capacity retention (83.5% of the initial values after 500 cycles at 0.5C).

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Molybdenum-containing polypyrrole self-supporting hollow flexible electrode for hydrogen peroxide detection in living cells

Cited by 16 publications

References 42 publications

Flexible Point-of-Care Electrodes for Ultrasensitive Detection of Bladder Tumor-Relevant miRNA in Urine

Flexible Point-of-Care Electrodes for Ultrasensitive Detection of Bladder Tumor-Relevant miRNA in Urine

Palladium Nanoparticles Stabilized by Lentinan with Enhanced Peroxidase‐like Activity for Sensitive Detection of H₂O₂

Synergy and Symbiosis Analysis of Capacity-Contributing Polypyrrole and Carbon-Coated Lithium Iron Phosphate Nanostructures for High-Performance Cathode Materials

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Molybdenum-containing polypyrrole self-supporting hollow flexible electrode for hydrogen peroxide detection in living cells

Cited by 16 publications

References 42 publications

Flexible Point-of-Care Electrodes for Ultrasensitive Detection of Bladder Tumor-Relevant miRNA in Urine

Flexible Point-of-Care Electrodes for Ultrasensitive Detection of Bladder Tumor-Relevant miRNA in Urine

Palladium Nanoparticles Stabilized by Lentinan with Enhanced Peroxidase‐like Activity for Sensitive Detection of H2O2

Synergy and Symbiosis Analysis of Capacity-Contributing Polypyrrole and Carbon-Coated Lithium Iron Phosphate Nanostructures for High-Performance Cathode Materials

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Palladium Nanoparticles Stabilized by Lentinan with Enhanced Peroxidase‐like Activity for Sensitive Detection of H₂O₂