Momordica charantia polysaccharides ameliorate oxidative stress, inflammation, and apoptosis in ethanol-induced gastritis in mucosa through NF-kB signaling pathway inhibition

Raish, Mohammad; Ahmad, Ajaz; Ansari, Mushtaq A.; Alkharfy, Khalid M.; Al-Jenoobi, Fahad I.; Jan, Basit L.; Al-Mohizea, Abdullah M.; Khan, Altaf; Ali, Naushad

doi:10.1016/j.ijbiomac.2018.01.008

Cited by 128 publications

(77 citation statements)

References 40 publications

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“…Therefore, we developed the gastric mucosa injury model in SD rats by gavage 5 mL/kg absolute ethanol and then rats were sacrificed one hour later in this study. The results showed that rats in ethanol group suffered severe gastric mucosal damage, exhibited edema, the mucosal surface was dark red, mucosal erosion, punctate, linear, cord-like and even flaky hemorrhage (Figure 1), and the incidence rate of injury was 100%, indicating that ethanol has caused gastric mucosa injury successfully, which is in accordance with previous studies [8,30]. However, gross observation showed that whey protein, omeprazole, and three WOPs groups had less gastric lesions and lower GUI values in comparison with the ethanol group, and the gastric mucosa injuries of rats in the WOPs-HG group was the lightest and significant and better than that of whey protein group or omeprazole group.…”

Section: Discussionsupporting

confidence: 90%

“…Most studies of ethanol-induced gastric mucosal damage have been performed in rodent models, but there is no generally accepted gavage dose of alcohol to date. According to multiple pieces of literature, absolute ethanol administered at a dose of 5 mL/kg in rodents was used more frequently, and the gastric mucosa injury can occur about 30 min and generally reach a peak 60 min after alcohol intake, which took into account differences in alcohol metabolism between rodents and humans [8,[25][26][27][28]. In pre-experiments, we found that the dose of absolute ethanol caused obvious damage to gastric mucosa with low mortality of rats.…”

Section: Discussionmentioning

confidence: 94%

“…The stomach was opened along the greater curvature, washed clean with cold saline, and blotted dry with filter paper, then the gross lesions of gastric tissue were observed and evaluated. The gastric ulcer index (GUI) was scored according to the Guth standard with some modifications [8,29,30], as shown in Table 1. Table 1.…”

Section: Microscopic Evaluation Of the Gastric Lesionsmentioning

confidence: 99%

“…At day 30, after the final administration, all rats were fasted for 24 h but given free access to water. Then rats were gavage with absolute ethanol at 5 mL/kg body weight to induce gastric ulceration based on reference to other research and pre-experiment [8,[25][26][27][28]. One hour later, animals were sacrificed, a blood sample was collected from the femoral artery and then centrifuged at 3000 rpm for 15 min to obtain serum and preserved at −80 • C; the stomach was immediately removed, and the gastric juice was collected into a tube and then the pH of gastric content was recorded with a digital pH meter.…”

Section: Introductionmentioning

confidence: 99%

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The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut (Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats

et al. 2020

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The study investigated the protective effect of walnut oligopeptides (WOPs) against ethanol-induced gastric injury using Sprague-Dawley (SD) rats. Rats were randomly divided into seven groups based on body weight (10/group), normal group, ethanol group, whey protein group (220 mg/kg body weight), omeprazole group (20 mg/kg body weight), and three WOPs groups (220, 440, 880 mg/kg body weight). After 30 days of treatment with WOPs, rats were given 5 mL/kg absolute ethanol by gavage to induce gastric mucosal injury. Gastric ulcer index (GUI) were determined and the following measured; gastric content pH, gastric mucin, endogenous pepsinogens (PG), prostaglandin E2 (PGE2), inflammatory cytokines, oxidative stress indicators, and the expression of apoptosis-related proteins were measured to evaluate the gastroprotective effect of WOPs. The results showed that the administration with WOPs markedly mitigated the hemorrhagic gastric lesions caused by ethanol in rats, and decreased the GUI, the gastric content pH, PG1, PG2, and NO levels, enhanced mucin and PGE2. Also, WOPs repressed gastric inflammation through the reduction of TNF-α, IL-6, IL-1β and increase IL-10 levels, and revealed antioxidant properties with the enhancement of superoxide dismutase, glutathione, and catalase activity, while reduction of malondialdehyde. Moreover, WOPs treatment significantly down-regulated Bax, caspase-3 and nuclear factor-κB p65 (NF-κB p65) expression, while up-regulating the expression of Bcl-2 and inhibitor kappa Bα (IκBα) protein. These results indicated that WOPs have protective effects against ethanol-induced gastric mucosal injury in rats through anti-inflammatory, anti-oxidation, and anti-apoptosis mechanisms.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 94%

Section: Microscopic Evaluation Of the Gastric Lesionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut (Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats

et al. 2020

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“…Stimuli and inhibitors for apoptosis may act at targets on the cell membrane via Fas and TNF receptors either in the cytoplasm or in the nucleus [28] . The ethanol-induced apoptosis of gastric mucosa could be ascribed to increases in TNF-α level, Bax, and caspase-3 activity [29] . A feature of malignancies is an alteration of cell turnover leading to cell hyperproliferation and to deregulation of apoptosis [30] .…”

Section: Discussionmentioning

confidence: 96%

Effect and mechanism of Acoritataninowii Rhizoma and Curcumae Radix on chronic gastritis: a network pharmacology study

Zhang¹,

Dai²,

Li³

et al. 2020

Preprint

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Background Chronic gastritis (CG) is an inflammatory disease which is one of the common diseases of the digestive system. To investigate the mechanisms of herbal pair Acoritataninowii Rhizoma(Shichangpu, AR) and Curcumae Radix༈Yujin, CR༉ in treatment of CG based on the network pharmacology. Methods The possible active ingredients and targets of AR-CR were obtained by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The UniProt database was used to query the human gene corresponding to each target protein. The genes related to CG were collected from the GeneCards database, the OMIM database, the DisGeNET database and the PharmGKB database. Intersected the target genes of AR-CR and CG, then protein-protein interaction(PPI) network was constructed by STRING website. The overlapped genes were subjected to gene ontology༈GO༉enrichment and Kyoto encyclopedia of genes and genomes༈KEGG༉pathway enrichment analyses by David. Results 45 intersection genes were obtained, and there were 40 targets in the PPI network for protein interaction, the kernel targets with Degree ≥ 10 included AKT1, TNF, JUN, MAPK3, MAPK8 and MAPK1. The Go enrichment analysis was mainly related to protein binding, enzyme binding, protein homodimerization activity, etc. The KEGG pathway enrichment analyses mainly involved the Pathways in cancer, TNF signaling pathway, Apoptosis, and VEGF signaling pathway. Conclusion AR-CR might delayed, blocked or reversed the atrophy, intestinal metaplasia, dysplasia and canceration of gastric mucosa by targeting key proteins and signal pathways,achieved the effect of the treatment of CG.

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Explore the effects of Shidan granules on chronic atrophic gastritis using LC–MS based plasma metabolomics study

Wang

Jun-quan

et al. 2021

Biomedical Chromatography

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Shidan granule (SDG), a traditional Chinese medicine in‐hospital preparation, has been demonstrated to exert good effects on chronic atrophic gastritis (CAG) in clinics. However, the underlying mechanism of SDG against CAG is still unclear. This study utilized an untargeted plasma metabolomics approach to explore the potential mechanism of SDG in CAG rats using LC–MS and pattern recognition analysis. The results indicated that SDG could effectively improve the biochemical indexes and pathology features of CAG rats. Nineteen potential biomarkers (variable importance in projection > 1 and P < 0.05) contributing to CAG progress were identified. After SDG intervention, 17 biomarkers were obviously restored to normal levels. Further metabolic pathway analysis showed that aspartate and glutamate metabolism, arachidonic acid metabolism, arginine and proline metabolism, and TCA cycle were the most related pathways for SDG treatment. Based on these findings, the main mechanisms of SDG against CAG might be attributed to the regulatory effects of energy balance, inflammatory suppression, and improvement in disturbed amino acid and lipid metabolism. This study provided information for the mechanism research of SDG against CAG and would promote its clinical application.

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Momordica charantia polysaccharides ameliorate oxidative stress, inflammation, and apoptosis in ethanol-induced gastritis in mucosa through NF-kB signaling pathway inhibition

Cited by 128 publications

References 40 publications

The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut (Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats

The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut (Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats

Effect and mechanism of Acoritataninowii Rhizoma and Curcumae Radix on chronic gastritis: a network pharmacology study

Explore the effects of Shidan granules on chronic atrophic gastritis using LC–MS based plasma metabolomics study

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